[1]李春晓,李怀臣.茵陈蒿汤调控Nrf-2/HO-1通路对慢性阻塞性肺疾病大鼠气道重塑的影响[J].陕西中医,2024,(8):1031-1035,1041.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.005]
　LI Chunxiao,LI Huaichen.Effect of Yinchenhao decoction on airway remodeling in rats with chronic obstructive pulmonary disease by regulating the Nrf-2/HO-1 pathway[J].,2024,(8):1031-1035,1041.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.005]
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茵陈蒿汤调控Nrf-2/HO-1通路对慢性阻塞性肺疾病大鼠气道重塑的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年8期
页码:
1031-1035,1041
栏目:
基础研究
出版日期:
2024-08-05

文章信息/Info

Title:
Effect of Yinchenhao decoction on airway remodeling in rats with chronic obstructive pulmonary disease by regulating the Nrf-2/HO-1 pathway
作者:
李春晓; 李怀臣
(山东中医药大学,山东 济南 250013)
Author(s):
LI Chunxiao; LI Huaichen
(Shandong University of Traditional Chinese Medicine,Jinan 250013,China)
关键词:
慢性阻塞性肺疾病;  茵陈蒿汤;  Nrf-2/HO-1通路;  气道重塑;  炎性反应;  氧化应激
Keywords:
Chronic obstructive pulmonary disease;  Yinchenhao decoction;  Nrf-2/HO-1 pathway;  Airway remodeling;  Inflammatory response;  Oxidative stress
分类号:
R 563
DOI:
DOI:10.3969/j.issn.1000-7369.2024.08.005
文献标志码:
A
摘要:
目的:探讨茵陈蒿汤调控抗氧化相关转录因子核因子相关因子2(Nrf-2)/亚铁血红素氧化酶1(HO-1)通路对慢性阻塞性肺疾病(COPD)大鼠气道重塑的影响。方法:通过注射脂多糖联合烟熏制备COPD大鼠,造模后随机分为模型组、茵陈蒿汤(4.41 g/kg)组、氨茶碱(2.3 mg/kg)组、茵陈蒿汤+抑制剂(4.41 g/kg茵陈蒿汤+30 mg/kg ML385)组,并以正常大鼠为对照组,检测大鼠肺功能[呼气峰流量(PEF)、第一秒用力呼气容积(FEV1)/用力肺活量(FVC)]; 评估血清白细胞介素(IL)-1β、IL-6水平及肺组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)水平; 检测肺组织病理学变化以及转化生长因子(TGF-β)、结缔组织生长因子(CTGF)阳性表达、金属基质蛋白酶(MMP)-2 mRNA、MMP-9 mRNA表达、Nrf-2、HO-1蛋白表达。结果:模型组FEV1/FVC、PEF、肺组织SOD、GSH-Px水平、Nrf-2、HO-1蛋白表达较对照组降低,血清IL-1β、IL-6、病理评分、肺组织TGF-β、CTGF、MMP-2 mRNA、MMP-9 mRNA表达增加(均P<0.05); 茵陈蒿汤或氨茶碱治疗后,FEV1/FVC、PEF、肺组织SOD、GSH-Px水平、Nrf-2、HO-1蛋白表达较模型组增加,血清IL-1β、IL-6、病理评分、肺组织TGF-β、CTGF、MMP-2 mRNA、MMP-9 mRNA表达显著降低(均P<0.05); 茵陈蒿汤联合ML385治疗后,FEV1/FVC、PEF、肺组织SOD、GSH-Px水平、Nrf-2、HO-1蛋白表达较茵陈蒿汤组降低,血清IL-1β、IL-6、病理评分、肺组织TGF-β、CTGF、MMP-2 mRNA、MMP-9 mRNA表达增加(均P<0.05)。结论:茵陈蒿汤可通过调控Nrf-2/HO-1通路改善COPD大鼠气道重塑。
Abstract:
Objective:To investigate the effect of Yinchenhao decoction on airway remodeling in rats with chronic obstructive pulmonary disease(COPD)by regulating the nuclear factor erythroid2-related factor(Nrf-2)/heme oxygenase-1(HO-1)pathway.Methods:COPD rats were prepared by injecting lipopolysaccharide combined with smoking.After modeling,they were randomly separated into model group,Yinchenhao decoction(4.41 g/kg)group,aminophylline(2.3 mg/kg)group,Yinchenhao decoction + inhibitor(4.41 g/kg Yinchenhao decoction + 30 mg/kg ML385)group,and normal rats were regarded as the reference group.Rat lung functions peak expiratory flow(PEF),first second forced expiratory volume(FEV1)/forced vital capacity(FVC)were measured.The levels of serum interleukin(IL)-1β,IL-6,lung tissue superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)were evaluated.The pathological changes in lung tissue,the positive expression of transforming growth factor-β(TGF-β)and connective tissue growth factor(CTGF),the expression of metalloproteinase-2 mRNA and MMP-9 mRNA,and the expression of Nrf-2 and HO-1 proteins were detected.Results:The FEV1/FVC,PEF,levels of SOD,GSH-Px,and expression of Nrf-2 and HO-1 proteins in lung tissue in model group were greatly lower than reference group,the serum IL-1β,IL-6,pathological score,lung tissue TGF-β,CTGF,MMP-2 mRNA,and MMP-9 mRNA expression were greatly higher(all P<0.05).However,after treatment with Yinchenhao decoction or aminophylline,the levels of FEV1/FVC,PEF,SOD,GSH-Px,Nrf-2,and HO-1 proteins in lung tissue were greatly higher than model group,the serum IL-1β,IL-6,pathological score,lung tissue TGF-β,CTGF,MMP-2 mRNA,and MMP-9 mRNA expression were greatly lower(all P<0.05).After Yinchenhao decoction + ML385 treatment,FEV1/FVC,PEF,SOD,GSH-Px levels in lung tissue,Nrf-2,and HO-1 protein expression were greatly lower than Yinchenhao decoction group,the serum IL-1β,IL-6,pathological score,lung tissue TGF-β,CTGF,MMP-2 mRNA,and MMP-9 mRNA expression were greatly higher(all P<0.05).Conclusion:Yinchenhao decoction improves airway remodeling in COPD rats by regulating the Nrf-2/HO-1 pathway.

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备注/Memo

备注/Memo:
基金项目:山东省自然科学基金资助项目(ZR2020KH013)
更新日期/Last Update: 2024-08-08