[1]马逢乐,等.电针耳迷走神经对断奶前母本隔离小鼠社会识别及前额叶皮层p-SYN1、神经可塑性的影响[J].陕西中医,2024,(10):1320-1325.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.005]
　MA Fengle,LI Chen,YUAN Wei,et al.Effects of electroacupuncture auricular vagus nerve on social recognition and p-SYN1,neuroplasticity in the prefrontal cortex in PMS mice[J].,2024,(10):1320-1325.[doi:DOI:10.3969/j.issn.1000-7369.2024.10.005]
点击复制

电针耳迷走神经对断奶前母本隔离小鼠社会识别及前额叶皮层p-SYN1、神经可塑性的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年10期
页码:
1320-1325
栏目:
基础研究
出版日期:
2024-10-05

文章信息/Info

Title:
Effects of electroacupuncture auricular vagus nerve on social recognition and p-SYN1,neuroplasticity in the prefrontal cortex in PMS mice
作者:
马逢乐1;  2;  3; 李 琛4; 袁 伟5; 董茜茜1; 2; 3
(1.陕西省颅颌面精准医学研究重点实验室,陕西 西安 710004; 2.陕西省牙颌疾病临床医学研究中心,陕西 西安 710004; 3.西安交通大学口腔医院牙体牙髓病科,陕西 西安 710004; 4.陕西省中医医院体检养生科,陕西 西安 710003; 5.陕西中医药大学医学科研实验中心,陕西 咸阳 712046)
Author(s):
MA Fengle; LI Chen; YUAN Wei; DONG Qianqian
(Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,Xi'an 710004,China)
关键词:
断奶前母本隔离;  耳迷走神经;  社会识别;  神经可塑性;  前额叶皮层;  电针
Keywords:
Preweaning maternal separation;  Auricular vagus nerve;  Social recognition;  Neuroplasticity;  Prefrontal cortex;  Electroacupuncture
分类号:
R 749
DOI:
DOI:10.3969/j.issn.1000-7369.2024.10.005
文献标志码:
A
摘要:
目的:研究电针耳迷走神经对断奶前母本隔离(PMS)小鼠社会识别和前额叶皮层(PFC)突触可塑性变化的影响,探讨电针耳迷走神经治疗因早期生活压力诱发的社会行为障碍的神经机制。方法:本实验采用C57BL/6小鼠,随机分为对照组、模型组和电针组,每组各10只。采用社会识别检测各组小鼠社会识别能力; 高尔基染色方法检测小鼠PFC树突棘密度变化; 实时荧光定量聚合酶链式反应(qPCR)检测PFC脑区神经营养因子(BDNF)、突触素1(SYN1)mRNA表达水平; 蛋白免疫印迹(Western blot)检测PFC脑区BDNF、SYN1、磷酸化突触素1(p-SYN1)蛋白的表达水平。结果:在社会识别实验中,与对照组比较,模型组小鼠在陌生鼠区域的停留时间下降(P<0.05); 与模型组比较,电针组小鼠在陌生鼠区域的停留时间上升(P<0.05)。高尔基染色实验中,与对照组比较,模型组小鼠树突棘数量和密度减少(P<0.05),电针组小鼠树突棘数量和密度增加(P<0.05); qPCR实验中,三组小鼠SYN1 mRNA表达差异无统计学意义; 与对照组比较,模型组小鼠BDNF mRNA下降(P<0.05); Western blot实验中,与对照组比较,模型组小鼠的BDNF、SYN1、p-SYN1蛋白的表达水平下降,与模型组相比,电针组小鼠的BDNF、SYN1、p-SYN1的表达水平上升。结论:电针能改善PMS介导的小鼠社会识别行为障碍,其机制可能与改善相关脑区神经元树突结构,提高前额叶皮层p-SYN1蛋白表达水平有关。
Abstract:
Objective:To investigate the effects of electroacupuncture auricular vagus nerve on the social recognition and the prefrontal cortex(PFC)synaptic plasticity in preweaning maternal separation(PMS)mice,and to explore the neural mechanism of electroacupuncture auricular vagus nerve therapy on cognitive disorder disorders induced by early life stress.Methods:Thirty C57BL/6 mice were used in this study and randomly divided into control group,PMS group and PMS+electroacupuncture group with 10 mice in each group.Social recognition test was conducted to evaluate the ability of social recognition.Golgi staining test was used to detect the dendritic spines density in the PFC.Quantitative polymerase chain reaction(qPCR)was used to detect the BDNF and SYN1 mRNA expression level in PFC.Western blot were used to detected the protein expression level of SYN1,p-SYN1 and BDNF in the PFC.Results:In social recognition test,compared with the control group,the time spent in the interaction zones of the model group were decreased(P<0.05),compared with the model group,the time spent in the interaction zone were increased of the electroacupuncture group(P<0.05).In golgi staining test,compared with control group,the number and density of dendritic spines were increased(P<0.05),compared with the model group,the number and density of dendritic spines were increased in the PFC(P<0.05).In qPCR test,compared with control group,the mRNA level of BDNF in model group was decreased(P<0.05),compared with model group,the mRNA level of BDNF in electroacupuncture group was increased in the PFC(P<0.05).No statistical differences were found in SYN1 mRNA expression among three groups in the PFC.In western blot test,compared with the control group,the BDNF,SYN1 and p-SYN1 protein expression levels in the model group were decreased(P<0.05),compared with the model group,the BDNF,SYN1 and p-SYN1 protein expression levels in the electroacupuncture group were increased in the PFC(P<0.05).Conclusion:Electroacupuncture improved the cognitive disorder caused by PMS in mice.The improvement of neuronal dendrite structure and the increase of p-SYN1 protein expression level in the PFC after electroacupuncture may the possible neuro mechanism.

参考文献/References:

[1] SALEH A,POTTER G G,MCQUOID D R,et al.Effects of early life stress on depression,cognitive performance and brain morphology[J].Psychol Med,2017,47(1):171-181.
[2] 吴楠,王强,李永丰,等.电针耳迷走神经对断奶前母本隔离模型小鼠焦虑、抑郁样行为的影响[J].康复学报,2022,32(6):502-509.
[3] TENKUMO C,OHTA K I,SUZUKI S,et al.Repeated maternal separation causes transient reduction in BDNF expression in the medial prefrontal cortex during early brain development,affecting inhibitory neuron development[J].Heliyon,2020,6(8):4781.
[4] 朱恩妮,吴超然,廖红.代谢型谷氨酸受体5在中枢神经系统疾病中的研究进展[J].中国药科大学学报,2021,52(6):751-758.
[5] BORRE Y E,O'KEEFFE G W,CLARKE G,et al.Microbiota and neurodevelopmental windows:Implications for brain disorders[J].Trends Mol Med,2014,20(9):509-518.
[6] JENKINS T A,NGUYEN J C,POLGLAZE K E,et al.Influence of tryptophan and serotonin on mood and cognition with a possible role of the gut-brain axis[J].Nutrients,2016,8(1):56.
[7] NADERIPOOR P,AMANI M,ABEDI A,et al.Alterations in the behavior,cognitive function,and BDNF level in adult male rats following neonatal blockade of GABA-A receptors[J].Brain Res Bull,2021,169:35-42.
[8] LEVY M J,BOULLE F,STEINBUSCH H W,et al.Neurotrophic factors and neuroplasticity pathways in the pathophysiology and treatment of depression[J].Psychopharma Cology(Berl),2018,235(8):2195-2220.
[9] XIAO X,XU X,LI F,et al.Anti-inflammatory treatment with β-asarone improves impairments in social interaction and cognition in MK-801 treated mice[J].Brain Res Bull,2019,150:150-159.
[10] GUO L,LIANG X,LIANG Z,et al.Electroacupuncture ameliorates cognitive deficit and improves hippocampal synaptic plasticity in adult rat with neonatal maternal separation[J].Evid Based Complement Alternat Med,2018,2018:2468105.
[11] 吴楠,王渊,王强,等.针刺耳迷走神经治疗抑郁症研究进展[J].陕西中医,2022,43(8):1140-1142,1146.
[12] WANG J Y,ZHANG Y,CHEN Y,et al.Mechanisms underlying antidepressant effect of transcutaneous auricular vagus nerve stimulation on CUMS model rats based on hippocampal α7nAchR/NF-κB signal pathway[J].J Neuroinflammation,2021,18(1):291.
[13] 张琼文,沙磊,张美珠,等.针灸治疗血管性认知障碍研究进展[J].陕西中医,2023,44(7):990-993.
[14] ESHKOOR S A,HAMID T A,MUN C Y,et al.Mild cognitive impairment and its management in older people[J].Clin Interv Aging,2015,10:687-693.
[15] 赤艺,程为平,温伟.中医治疗痴呆病研究进展[J].陕西中医,2020,41(10):1507-1509.
[16] 徐光镇,刘继洪,李可,等.耳穴压丸法联合耳穴按摩法治疗轻度认知障碍的临床研究[J].现代中西医结合杂志,2020,29(6):575-578,584.
[17] 刘征,梁爽,毕海洋,等.经颅重复针刺激治疗帕金森病轻度认知障碍的疗效观察[J].广州中医药大学学报,2020,37(11):2152-2156.
[18] 陈瑜凡,王燕平,荣培晶,等.耳针疗法对糖尿病前期干预的探讨[J].世界中医药,2021,16(24):3629-3632.
[19] LIU C H,YANG M H,ZHANG G Z,et al.Neural networks and the anti-inflammatory effect of transcutaneous auricular vagus nerve stimulation in depression[J].J Neuroinflammation,2020,17(1):54.
[20] LAMPROS M,VLACHOS N,ZIGOURIS A,et al.Transcutaneous vagus nerve stimulation(t-VNS)and epilepsy:A systematic review of the literature[J].Seizure,2021,91:40-48.
[21] 张帅,何家恺,赵亚楠,等.经皮耳穴-迷走神经电刺激对原发性失眠患者默认网络的调制作用[J].中国针灸,2022,42(4):363-368,406.
[22] 荣培晶,张悦,李少源,等.经皮耳穴迷走神经刺激治疗脑及相关疾病的现状与展望[J].世界科学技术-中医药现代化,2019,21(9):1799-1804.
[23] LUPIEN S J,MCEWEN B S,GUNNAR M R,et al.Effects of stress throughout the lifespan on the brain,behaviour and cognition[J].Nat Rev Neurosci,2009,10(6):434-445.
[24] SAKAMOTO T,YASHIMA J.Prefrontal cortex is necessary for long-term social recognition memory in mice[J].Behav Brain Res,2022,435:114051.
[25] ZHANG H,ZABLAH Y,ZHANG H,et al.Inhibition of Rac1 in ventral hippocampal excitatory neurons improves social recognition memory and synaptic plasticity[J].Front Aging Neurosci,2022,14:914491.
[26] ZHANG H,ZABLAH Y,LIU A,et al.Overexpression of LIMK1 in hippocampal excitatory neurons improves synaptic plasticity and social recognition memory in APP/PS1 mice[J].Mol Brain,2021,14(1):121.
[27] LI Y,SUN Z,CAO Q,et al.Role of amyloid β protein receptors in mediating synaptic plasticity[J].Biomed Rep,2017,6(4):379-386.
[28] 高静,赖名殷,梅氏清心,等.电针对抑郁大鼠前额叶脑源性神经营养因子/哺乳动物雷帕霉素复合物1通路及突触可塑性的影响[J].针刺研究,2022,47(1):15-20,32.
[29] NOTARAS M,BUUSE M.Neurobiology of BDNF in fear memory,sensitivity to stress,and stress-related disorders[J].Mol Psychiatry,2020,25(10):2251-2274.
[30] VAN BOXELAERE M,CLEMENTS J,CALLAERTS P,et al.Unpredictable chronic mild stress differentially impairs social and contextual discrimination learning in two inbred mouse strains[J].PLoS One,2017,12(11):188537.
[31] 袁丹,徐思雨,甄伟兰,等.丁苯酞联合尼麦角林治疗血管性痴呆疗效及对患者血清神经视锥蛋白样蛋白1、脑源性神经营养因子水平的影响[J].陕西医学杂志,2023,52(9):1233-1236.

备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2022JM-523); 陕西省教育厅科研计划项目(重点实验室项目)(21JS021)
更新日期/Last Update: 2024-10-08