[1]孙春英,张 鼎,陈 炜,等.温肺降浊方对血管性痴呆大鼠线粒体外膜蛋白的影响[J].陕西中医,2024,(11):1449-1454.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.002]
　SUN Chunying,ZHANG Ding,CHEN Wei,et al.Explore effect of Wenfei Jiangzhuo formula on the expression of mitochondrial outer membrane protein in vascular dementia model rats[J].,2024,(11):1449-1454.[doi:DOI:10.3969/j.issn.1000-7369.2024.11.002]
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温肺降浊方对血管性痴呆大鼠线粒体外膜蛋白的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1449-1454
栏目:
基础研究
出版日期:
2024-11-05

文章信息/Info

Title:
Explore effect of Wenfei Jiangzhuo formula on the expression of mitochondrial outer membrane protein in vascular dementia model rats
作者:
孙春英1; 张 鼎1; 陈 炜2; 李方存2; 张 玲1; 周珂青1; 向军军1; 胡跃强2
(1.广西中医药大学研究生院,广西 南宁 530001; 2.广西中医药大学第一附属医院,广西 南宁 530023)
Author(s):
SUN Chunying; ZHANG Ding; CHEN Wei; LI Fangcun; ZHANG Ling; ZHOU Keqing; XIANG Junjun; HU Yueqiang
(Graduate School of Guangxi University of Traditional Chinese Medicine,Nanning 530001,China)
关键词:
血管性痴呆;  线粒体动力学平衡;  温肺降浊方;  FUN14结构域包含蛋白1;  线粒体分裂蛋白1
Keywords:
Vascular dementia;  Mitochondrial kinetic equilibrium;  Wenfei Jiangzhuo formula;  FUNDC1;  Fis1
分类号:
R 74
DOI:
DOI:10.3969/j.issn.1000-7369.2024.11.002
文献标志码:
A
摘要:
目的:探讨温肺降浊方对血管性痴呆(VaD)模型大鼠FUN14结构域包含蛋白1(FUNDC1)、线粒体分裂蛋白1(Fis1)、线粒体融合蛋白1(Mfn1)、线粒体融合蛋白2(Mfn2)等线粒体外膜蛋白的影响,探究本方可能的作用机制。方法:将24只大鼠随机分为假手术组、模型组、阳性对照组、温肺降浊方组,每组各6只。采用双侧颈总动脉永久性结扎法制备VaD大鼠模型,温肺降浊方组予以温肺降浊方药液,阳性对照组予以盐酸多奈哌齐。Morris水迷宫行为学测试检测各组大鼠认知水平,HE染色法检测各组大鼠海马组织病理形态,免疫荧光化学染色检测大鼠海马中FUNDC1与 Fis1阳性细胞数表达,Western blotting检测FUNDC1、Fis1、Mfn1与Mfn2蛋白表达水平。结果:与假手术组相比,模型组大鼠逃避潜伏期延长、穿越平台次数减少(P<0.05),细胞破碎,核缩小,水肿化,海马中FUNDC1与Fis1阳性细胞表达量上升,FUNDC1、Fis1蛋白上升,Mfn1/2蛋白降低(P<0.05)。与模型组比较,阳性对照组和温肺降浊方组逃避潜伏期缩短、穿越平台次数增加(P<0.05),细胞核逐渐趋于正常,水肿化减少,FUNDC1与 Fis1阳性细胞数及蛋白下降,Mfn1/2蛋白升高(P<0.05)。结论:温肺降浊方通过下调FUNDC1、Fis1蛋白水平,上调Mfn1/Mfn2蛋白维持线粒体动力学平衡,减轻海马神经元损伤,发挥脑保护作用。
Abstract:
Objective:To investigate effects of Wenfei Jiangzhuo formula on the expression of mitochondrial outer membrane proteins such as FUN14 structural domain-containing protein 1(FUNDC1),mitochondrial fission protein 1(Fis1),mitochondrial fusion protein 1(Mfn1),mitochondrial fusion protein 2(Mfn2)and other mitochondrial outer membrane proteins in rats with vascular dementia(VaD)model,in order to explore the possible mechanism of action of the formula.Methods:Twenty-four rats were randomly divided into the sham-operation group,model group,positive control group,and Wenfei Jiangzhuo formula group,with six rats in each group.The rat model of VaD were prepared by permanent ligation of bilateral common carotid arteries,and the Wenfei Jiangzhuo formula group were treated with Wenfei Jiangzhuo formula liquid,and the positive control group were treated with Donepezil hydrochloride.The cognitive level of the rats was detected by Morris water maze behavioral test,the histopathological morphology of hippocampus in the groups were detected by HE staining,the expression of FUNDC1 and Fis1-positive cell number was detected by immunofluorescent staining.The expression levels of FUNDC1,Fis1,Mfn1 and Mfn2 proteins were detected by Western blotting.Results:Compared with the sham-operated group,the model group rats had longer evasion latency,reduced number of platform crossings(P<0.05),cell fragmentation,nuclear shrinkage,oedema,an increase in the expression of FUNDC1 and Fis1-positive cells in the hippocampus,increase in the expression of FUNDC1 and Fis1 proteins,and reduction in the expression of Mfn1/2 proteins(P<0.05).Compared with the model group,the positive control group and the Wenfei Jiangzhuo formula group evaded the shortening of latency and the increase in the number of crossing the plateau(P<0.05),the nuclei of the cells gradually tended to be normalised,the oedematousness was reduced,the number and protein expression of the FUNDC1 and Fis1-positive cells were decreased,and the protein expression of Mfn1/2 was elevated(P<0.05).Conclusion:Wenfei Jiangzhuo formula may maintain the balance of mitochondrial dynamics,attenuate hippocampal neuronal damage and exert cerebroprotective effects by down-regulating the expression levels of FUNDC1 and Fis1 proteins and up-regulating the expression of Mfn1/Mfn2 proteins.

参考文献/References:

[1] 张敬,陶晓杰,马晓维,等.从脏腑论治血管性痴呆的研究进展[J].湖北中医杂志,2024,46(1):59-63.
[2] 李超,熊兰,卢元明.银杏叶提取物联合茴拉西坦治疗血管性痴呆疗效观察[J].陕西医学杂志,2023,52(6):737-740.
[3] 阙彬福,曾小连,马观福生,等.灯盏生脉胶囊联合针刺对脑梗死后血管性痴呆患者的临床疗效[J].中成药,2024,46(5):1529-1532.
[4] 徐倩丽,庄玲玲,卢宏磊,等.中医外治法治疗轻度认知功能障碍的作用机制研究进展[J].中医药信息,2024,41(8):82-87.
[5] WU L,FENG X T,HU Y Q,et al.Global gene expression profile of the hippocampus in a rat model of vascular dementia[J].Tohoku J Exp Med,2015,237(1):57-67.
[6] FLIPPO K H,STRACK S.Mitochondrial dynamics in neuronal injury development and plasticity[J].J Cell Sci,2017,130(4):671-681.
[7] RODRIGUES T,FERRAZ L S.Therapeutic potential of targeting mitochondrial dynamics in cancer[J].Biochem Pharmacol,2020,182:114282.
[8] LIU L,FENG D,CHEN G,et al.Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells[J].Nat Cell Biol,2012,14(2):177-185.
[9] MA F,LI H,HUO H,et al.N-acetyl-L-cysteine alleviates FUNDC1-mediated mitophagy by regulating mitochondrial dynamics in type 1 diabetic nephropathy canine[J].Life Sci,2023,15(1):121278.
[10] 姜明贺,张鼎,朱欢欢,等.温肺降浊方对血管性痴呆大鼠海马神经元的作用机制[J].陕西中医,2024,45(3):291-296.
[11] 詹晨阳,黄秋霞,张婷,等.基于微生物-肠-脑轴理论探讨温肺降浊方治疗血管性痴呆症的作用机制[J].中医临床研究,2023,15(9):77-82.
[12] 宋晨曦,张鼎,胡芷涵,等.温肺降浊方对血管性痴呆大鼠 ERK1/2信号通路相关蛋白的影响[J].陕西中医,2024,45(2):171-175,186.
[13] 张鼎,胡芷涵,宋晨曦,等.温肺降浊方通过Aβ25-35诱导PC12细胞损伤对PINK1/PARKIN介导的线粒体自噬的影响[J].中药材,2024,47(2):448-454.
[14] 孙旭,王蕾,赵婧,等.血管性痴呆动物模型研究概述[J].广州中医药大学学报,2022,39(10):2465-2470.
[15] 陈业文,陈炜,吴林,等.基于肠道微生物探讨从脾胃论治血管性痴呆[J].西部中医药,2021,34(4):145-149.
[16] 王晋平,唐农,刘布谷,等.唐农教授运用“扶阳思想”次第治疗痴呆病的经验[J].中华中医药学刊,2016,34(5):1062-1064.
[17] 胡跃强,向军军,赖菁菁,等.从线粒体相关凋亡蛋白探讨温肺降浊方对VD大鼠学习记忆能力的影响[J].中医药通报,2017,16(2):59-62.
[18] 胡跃强,唐农,吴林,等.温肺降浊方联合盐酸多奈哌齐片治疗血管性痴呆的疗效及其机制[J].现代中西医结合杂志,2019,28(13):1378-1381.
[19] YOO S M,JUNG Y K.A molecular approach to mitophagy and mitochondrial dynamics[J].Mol Cells,2018,41(1):18-26.
[20] VASILEIOU P V S,EVANGELOU K,VLASIS K,et al.Mitochondrial homeostasis and cellular senescence [J].Cells,2019,8(7):686.
[21] VASQUEZ-TRINCADO C,GARCIA-CARVAJAL I,PENNANEN C,et al.Mitochondrial dynamics mitophagy and cardiovascular disease[J].J Physiol,2016,594:509-525.
[22] TILOKANI L,NAGASHIMA S,PAUPE V,et al.Mitochondrial dynamics:Overview of molecular mechanisms[J].Essays Biochem,2018,62(3):341-360.
[23] GAO S,HU J.Mitochondrial fusion:The machineries in and out[J].Trends Cell Biol,2021,31(1):62-74.
[24] CHEN M,CHEN Z,WANG Y,et al.Mitophagy receptor FUNDC1 regulates mitochondrial dynamics and mitophagy[J].Autophagy,2016,12(4):689-702.
[25] RAMBOLD A S,PEARCE E L.Mitochondrial dynamics at the interface of immune cell metabolism and function[J].Trends Immunol,2018,39(1):6-18.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82260904); 国家中医药管理局高水平重点学科建设项目(ZYYZDXK-2023166); 广西中医脑病临床研究中心项目(桂科AD20238028); 广西中医药大学“岐黄工程”高层次人才团队项目(202410); 广西中医药重点学科建设项目(GZXK-Z-20-13); 广西中医药大学博士研究生创新项目(YCBXJ2023010)
更新日期/Last Update: 2024-11-11