[1]瞿 胤,陆 宏,方臣阳,等.促愈熏洗方通过SOCS1介导JAK/STAT通路调控巨噬细胞M2型极化的机制研究[J].陕西中医,2024,(12):1587-1591,1597.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.001]
　QU Yin,LU Hong,FANG Chenyang,et al.Mechanism of Cuyu Xunxi formula regulating M2-type polarization of macrophages by SOCS1 mediating JAK/STAT pathways[J].,2024,(12):1587-1591,1597.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.001]
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促愈熏洗方通过SOCS1介导JAK/STAT通路调控巨噬细胞M2型极化的机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
1587-1591,1597
栏目:
基础研究
出版日期:
2024-12-05

文章信息/Info

Title:
Mechanism of Cuyu Xunxi formula regulating M2-type polarization of macrophages by SOCS1 mediating JAK/STAT pathways
作者:
瞿 胤; 陆 宏; 方臣阳; 韩 晔; 杨 巍; 郑 德
(上海中医药大学附属曙光医院肛肠科,上海 201202)
Author(s):
QU Yin; LU Hong; FANG Chenyang; HAN Ye; YANG Wei; ZHENG De
(Department of Proctology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201202,China)
关键词:
促愈熏洗方;  创面修复;  巨噬细胞极化;  M1/M2表型;  细胞因子信号转导抑制蛋白1;  Janus激酶/信号转导和转录激活因子信号通路
Keywords:
Cuyu Xunxi formula;  Wound repair;  Macrophage polarization;  M1/M2 phenotype;  Suppressor of cytokine signaling 1;  Janus kinase/signal transduction and activator of transcription signaling pathway
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2024.12.001
文献标志码:
A
摘要:
目的:探究促愈熏洗方通过细胞因子信号转导抑制蛋白1(SOCS1)介导Janus激酶/信号转导和转录激活因子(JAK/STAT)通路调控巨噬细胞M2型极化的作用机制。方法:用白细胞介素-4(IL-4)刺激巨噬细胞系RAW264.7,诱导巨噬细胞M2极化,RT-qPCR与Western blot检测SOCS1、SOCS2和SOCS3表达,筛选效应因子。脂多糖(LPS)诱导巨噬细胞M1极化,分别用促愈熏洗方、siSOCSs处理细胞,检测M2型巨噬细胞表面标记分子(CD206、CD163)、相关炎症因子[IL-10、IL-13和转化生长因子β(TGF-β)]及JAK/STAT信号通路(STAT3、STAT6)表达。结果:与对照组比较,IL-4组细胞中SOCS1 mRNA及蛋白表达升高,SOCS3 mRNA及蛋白表达降低(均P<0.05),其中SOCS1表达变化最为显著。与对照组比较,IL-4组p-STAT3、p-STAT6、CD206和CD163蛋白表达及IL-10、IL-13、TGF-β含量升高(均P<0.05); 与IL-4组比较,IL-4+siSOCS1组p-STAT3、p-STAT6、CD206、CD163及IL-10、IL-13、TGF-β降低(均P<0.05)。与对照组比较,LPS组CD206、CD163、IL-10、IL-13、TGF-β、p-STAT3、p-STAT6降低(均P<0.05); 与LPS组比较,LPS+促愈熏洗方组SOCS1、CD206、CD163、IL-10、IL-13、TGF-β、p-STAT3、p-STAT6升高(均P<0.05); 与LPS+促愈熏洗方组比较,LPS+促愈熏洗方+siSOCS1组SOCS1、CD206、CD163、IL-10、IL-13、TGF-β、p-STAT3、p-STAT6降低(均P<0.05)。结论:促愈熏洗方通过SOCS1激活JAK/STAT通路诱导巨噬细胞M2型极化。
Abstract:
Objective:To explore the mechanism of Cuyu Xunxi formula regulating M2-type polarization of macrophages by suppressor of cytokine signaling 1(SOCS1)mediating Janus kinase/signal transduction and activator of transcription(JAK/STAT)pathways.Methods:Macrophage line RAW264.7 was stimulated with interleukin-4(IL-4)to induce M2 polarization of macrophages.The expressions of SOCS1,SOCS2 and SOCS3 were detected by RT-qPCR and Western blot to screen effecting factors.M1 polarization of macrophages was induced by lipopolysaccharide(LPS),and cells were treated with Cuyu Xunxi formula and siSOCSs.The expressions of marker molecules(CD206,CD163)on M2-type macrophages surface,related inflammatory factors(IL-10,IL-13,TGF-β)and JAK/STAT signaling pathways(STAT3,STAT6)were detected.Results:Compared with control group,expressions of SOCS1 mRNA and protein were increased,while expressions of SOCS3 mRNA and protein were decreased in IL-4 group(all P<0.05),and the changes of SOCS1 expression were the most significant.Compared with control group,expressions of p-STAT3,p-STAT6,CD206 and CD163 proteins,and levels of IL-10,IL-13 and TGF-β were increased in IL-4 group(all P<0.05).Compared with IL-4 group,expressions of p-STAT3,p-STAT6,CD206 and CD163 proteins,and levels of IL-10,IL-13 and TGF-β were decreased in IL-4+siSOCS1 group(all P<0.05).Compared with control group,CD206,CD163,IL-10,IL-13,TGF-β,p-STAT3 and p-STAT6 were decreased in LPS group(all P<0.05).Compared with LPS group,SOCS1,CD206,CD163,IL-10,IL-13,TGF-β,p-STAT3 and p-STAT6 were increased in LPS+Cuyu Xunxi formula group(all P<0.05).Compared with LPS+Cuyu Xunxi formula group,SOCS1,CD206,CD163,IL-10,IL-13,TGF-β,p-STAT3 and p-STAT6 were decreased in LPS+Cuyu Xunxi formula+siSOCS1 group(all P<0.05).Conclusion:Cuyu Xunxi formula can induce M2-type polarization of macrophages by SOCS1 activating JAK/STAT pathways.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年基金资助项目(82104868); 国家中医药管理局第七批全国老中医药专家学术经验继承工作项目[国中医药人教函(2022)76号]; 上海市科研计划项目(21Y11923200); 上海市卫生健康委员会科研项目(202340278); 上海中医药大学附属医院“临床研究型骨干人才培养计划”项目(2023LCRC07)
更新日期/Last Update: 2024-12-05