[1]杨上松,张庭瑞,明雄珍,等.清瘟解热方通过调节巨噬细胞糖酵解干预病毒性肺炎湿热证实验研究[J].陕西中医,2024,(12):1598-1603.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.003]
　YANG Shangsong,ZHANG Tingrui,MING Xiongzhen,et al.Experimental study on Qingwen Jiere recipe interfering with damp-heat syndrome of viral pneumonia by regulating glycolysis of macrophage[J].,2024,(12):1598-1603.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.003]
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清瘟解热方通过调节巨噬细胞糖酵解干预病毒性肺炎湿热证实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
1598-1603
栏目:
基础研究
出版日期:
2024-12-05

文章信息/Info

Title:
Experimental study on Qingwen Jiere recipe interfering with damp-heat syndrome of viral pneumonia by regulating glycolysis of macrophage
作者:
杨上松1; 2; 张庭瑞1; 3; 明雄珍1; 刘常媛1; 温伟波1; 李 钦1
(1.云南中医药大学,云南 昆明 650500; 2.玉溪职业技术学院,云南 玉溪 653100; 3.仙桃市中医院,湖北 仙桃 433000)
Author(s):
YANG Shangsong; ZHANG Tingrui; MING Xiongzhen; LIU Changyuan; WEN Weibo; LI Qin
(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China)
关键词:
病毒性肺炎;  清瘟解热方;  湿热证;  巨噬细胞;  糖酵解;  小鼠
Keywords:
Viral pneumonia;  Qingwen Jiere recipe;  Damp-heat syndrome;  Macrophage;  Glycolysis;  Mice
分类号:
R 563.1
DOI:
DOI:10.3969/j.issn.1000-7369.2024.12.003
文献标志码:
A
摘要:
目的:探索清瘟解热方通过调节巨噬细胞糖酵解治疗病毒性肺炎湿热证的作用机制。方法:通过采用高脂饮食和气候箱湿热暴露14 d叠加气管滴注Poly(I:C)复合造模法,建立病毒性肺炎湿热证肺损伤小鼠模型,并将小鼠随机分为正常组、模型组和清瘟解热方(QWJR)低、中、高剂量组[予QWJR灌胃16.29、32.58、65.17 g/(kg·d)]、阳性药物组[腹腔注射地塞米松0.5 mg/(kg·d)]。观察小鼠湿热证候并评分,观测小鼠体重、体温、食量、饮水量及肺指数; ELISA法检测各组肺泡灌洗液中(BALF)中炎症因子IL-17、IL-23水平及肺泡巨噬细胞中己糖激酶(HK)和磷酸果糖激酶(PFK)活性; HE染色观察肺组织、舌组织病理学变化; RT-qPCR及Western blot检测缺氧诱导因子-1α(HIF-1α)及丙酮酸激酶M2(PKM2)相关基因及蛋白表达。结果:模型组湿热证候积分、肛温、肺指数、IL-17和IL-23水平、HK和PFK活性、肺损伤病理评分、舌组织黏膜层厚度,HIF-1α、PKM2 mRNA及蛋白表达较正常组增加,体重、食量、饮水量降低(均P<0.05); 与模型组比较,地塞米松组和QWJR-H组小鼠湿热证候积分、肺指数、IL-17和IL-23水平、HK和PFK活性、肺损伤病理评分、舌组织黏膜层厚度,肺组织HIF-1α、PKM2 mRNA及蛋白表达降低,体重、食量、饮水量增加(均P<0.05),且以高剂量清瘟解热方干预效果最好(均P<0.05)。结论:清瘟解热方可能通过调控巨噬细胞糖酵解、抑制过度炎症反应发挥对病毒性肺炎湿热证小鼠的保护作用。
Abstract:
Objective:To explore the mechanism of Qingwen Jiere recipe in treating damp-heat syndrome of viral pneumonia by affecting glycolysis of macrophage.Methods:A mouse model of lung injury with damp-heat syndrome of viral pneumonia was established by using high-fat diet and humid-heat exposure in climate box for 14 days combined with tracheal instillation of Poly(I:C).The mice were randomly divided into model group,low,medium and high dose QWJR groups [16.29,32.58,65.17 g/(kg·d)] and positive drug group [0.5 mg/(kg·d)by intraperitoneal injection of dexamethasone].The damp-heat syndrome of mice was observed and scored.The body weight,body temperature,food intake,water intake and lung index of mice were observed.The levels of inflammatory factors IL-17 and IL-23 in BALF and the activities of HK and PFK in alveolar macrophages were detected by ELISA.HE staining was used to observe the pathological changes of lung tissue and tongue tissue.The expression of glycolytic key factor HIF-1α and glycolytic key rate-limiting enzyme PKM2 related genes and proteins were detected by RT-qPCR and Western blot.Results:The scores of damp-heat syndrome,rectal temperature,lung index,IL-17,IL-23 levels,HK,PFK activity,pathological score of lung injury,mucosal thickness of tongue tissue,HIF-1α,PKM2 mRNA and protein expression in the model group were higher than those in the normal group,while body weight,food intake and water intake were lower(all P<0.05).Compared with the model group,the scores of damp-heat syndrome,lung index,levels of IL-17 and IL-23,HK and PFK activities,pathological scores of lung injury,mucosal thickness of tongue tissue,mRNA and protein expression of HIF-1α and PKM2 in lung tissue were decreased,and body weight,food intake and water intake were increased in dexamethasone group and Qingwen Jiere recipe high-dose group(all P<0.05),and the intervention effect of Qingwen Jiere recipe high-dose group was the best(all P<0.05).Conclusion:Qingwen Jiere formula may play a protective role in mice with damp-heat syndrome of viral pneumonia by regulating macrophage glycolysis and inhibiting excessive inflammatory response.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金地区基金资助项目(82260930,82060864); 国家重点研发计划项目(2023YFF0724803); 国家中医药管理局新型冠状病毒感染中医药应急专项课题(2023ZYLCYJ02-24); 国家中医药多学科交叉创新团队项目(ZYYCXTD-D-202201); 国家级大学生创新训练计划项目(202310680002); 云南省科学技术厅中医联合专项(202101AZ070001-020); 云南省科技厅重点研发计划项目(202103AC100005); 云南中医药大学校院联合基金资助项目(XYLH202329); 云南省教育厅科学研究项目(2023Y0440); 云南省中医学一流学科创新科研基金资助项目(ZYXZD202401)
更新日期/Last Update: 2024-12-05