[1]陈真珍,李日著,谢福周,等.补阳壮通饮通过调控细胞增殖和线粒体自噬保护星形胶质细胞机制研究[J].陕西中医,2024,(12):1604-1608.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.004]
　CHEN Zhenzhen,LI Rizhu,XIE Fuzhou,et al.Study on the protective mechanism of Buyang Zhuangtong Yin on astrocytes by regulating cell proliferation and mitochondrial autophagy[J].,2024,(12):1604-1608.[doi:DOI:10.3969/j.issn.1000-7369.2024.12.004]
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补阳壮通饮通过调控细胞增殖和线粒体自噬保护星形胶质细胞机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
1604-1608
栏目:
基础研究
出版日期:
2024-12-05

文章信息/Info

Title:
Study on the protective mechanism of Buyang Zhuangtong Yin on astrocytes by regulating cell proliferation and mitochondrial autophagy
作者:
陈真珍1; 李日著2; 谢福周3; 林富生3; 李 岩4; 王凯华4; 黄龙坚3; 5
(1.广西中医药大学,广西 南宁 530200; 2.右江民族医学院附属医院,广西 百色 533000; 3.右江民族医学院,广西 百色 533000; 4.广西中医药大学附属国际壮医医院,广西 南宁 530201; 5.广西高校右江流域特色民族药研究重点实验室,广西 百色 533000)
Author(s):
CHEN Zhenzhen; LI Rizhu; XIE Fuzhou; LIN Fusheng; LI Yan; WANG Kaihua; HUANG Longjian
(Guangxi University of Chinese Medicine,Nanning 530200,China)
关键词:
补阳壮通饮;  糖氧剥夺/再灌注;  含药血清;  抗炎;  线粒体自噬;  p38-MAPK
Keywords:
Buyang Zhuangtong Yin;  Glucose oxygen deprivation/reperfusion;  Medicated serum;  Anti inflammation;  Mitochondrial autophagy;  p38-MAPK
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2024.12.004
文献标志码:
A
摘要:
目的:初步探讨补阳壮通饮对脑缺血再灌注损伤(CIRI)的神经保护作用机制。方法:建立糖氧剥夺/再灌注(OGD/Rep)细胞模型,分为正常组(NC)、低剂量模型对照组(OGD-L)、中剂量模型对照组(OGD-M)、高剂量模型对照组(OGD-H)、补阳壮通饮低剂量组(BYZTY-L)、补阳壮通饮中剂量组(BYZTY-M)和补阳壮通饮高剂量组(BYZTY-H)。大鼠连续灌胃补阳壮通饮7 d以制备含药血清。利用含药血清对上述分组细胞进行干预培养3 d。MTT法检测细胞增殖活力; 流式细胞仪检测细胞凋亡率变化; ELISA法检测炎症指标(TNF-α、IL-6)变化; Western blot检测线粒体自噬相关蛋白LC3、Beclin-1、P62和磷酸化p38-MAPK蛋白表达变化。结果:补阳壮通饮干预可增强模型组大鼠原代皮层神经细胞的增殖活力,抑制其凋亡,促进LC3、Beclin-1和磷酸化p38-MAPK蛋白的表达,抑制P62蛋白表达,且表现一定的剂量和时间效应。结论:补阳壮通饮对OGD/Rep损伤星形胶质细胞具有保护作用,机制可能与增强细胞增殖活力和线粒体自噬、抑制凋亡和炎症反应有关,p38-MAPK通路也可能参与其中。
Abstract:
Objective:This study aims to explore the molecular mechanism of the neuroprotective effect of Buyang Zhuangtong Yin on cerebral ischemia-reperfusion injury.Methods:Established oxygen and glucose deprivation/reperfusion(OGD/Rep)cell models,and they were divided into normal group(NC),low-dose model control group(OGD-L),medium dose model control group(OGD-M),high-dose model control group(OGD-H),low-dose Buyang Zhuangtong Yin group(BYZTY-L),medium dose Buyang Zhuangtong Yin group(BYZTY-M),and high-dose Buyang Zhuangtong Yin group(BYZTY-H).Rats were continuously gavaged with Buyang Zhuangtong Yin for 7 days to prepare medicated serum.Using medicated serum to intervene and cultivate the above-mentioned grouped cells for 3 days.MTT assay was used to detect cell proliferation activity.Flow cytometry was used to detect changes in cell apoptosis rate.ELISA was used to detect the inflammatory markers(TNF-α and IL-6)changes.Western blot was used to detect the mitochondrial autophagy related proteins(LC3,Beclin-1,P62 and p-p38-MAPK)changes.Results:Buyang Zhuangtong Yin can enhance the proliferation activity of primary cortical neurons,inhibit apoptosis,and induce the expression of LC3,Beclin-1 and p38-MAPK,and inhibit the expression of P62,exhibit a certain dose and time effects.Conclusion:Buyang Zhuangtong Yin has a protective effect on astrocytes damaged by OGD/Rep injury,which may be related to enhancing cell proliferation activity and mitochondrial autophagy,inhibiting apoptosis and inflammatory response,and also the p38-MAPK pathway may also be involved.

参考文献/References:

[1] 李岩,王凯华,覃启京,等.西药联合壮医壮通饮治疗缺血性中风38例临床观察[J].中国民族民间医药,2020,29(22):103-106,115.
[2] 李岩,覃启京,梁慧荟,等.壮通饮抑制NADPH氧化酶2对糖氧剥夺诱导星形胶质细胞损伤的保护作用[J].亚太传统医药,2020,16(9):17-21.
[3] 陈娟,暴军,张颖,等.补阳还五汤对大鼠脑缺血再灌注损伤后脑组织铁转运相关蛋白的影响[J].中华中医药杂志,2023,38(11):5231-5236.
[4] 奚宇杰,朱晨,常菲,等.补阳还五汤治疗气虚血瘀型缺血性脑中风临床研究[J].新中医,2022,54(21):47-50.
[5] 马冰冰.补阳壮通饮对中风病气虚血瘀证患者疗效和神经丝轻链蛋白水平影响的临床研究[D].南宁:广西中医药大学,2022.
[6] 王亚南,陈真珍,王凯华,等.补阳壮通饮对脑缺血再灌注损伤大鼠的神经保护作用及其可能机制研究[J].医学研究杂志,2024,53(4):40-45.
[7] 张鼎,向军军,李方存,等.温阳复元方对脑缺血再灌注损伤大鼠的治疗机制[J].陕西中医,2023,44(3):279-284.
[8] YUAN Q,YUAN Y,ZHENG Y,et al.Anti-cerebral ischemia reperfusion injury of polysaccharides:A review of the mechanisms[J].Biomed Pharmacother,2021,137:111303.
[9] 张丽丽.活血开窍汤对大鼠脑缺血再灌注损伤NO含量及细胞凋亡的影响[D].天津:天津医科大学,2013.
[10] XU Y,ZHANG J,GAO F,et al.Engeletin alleviates cerebral ischemia reperfusion-induced neuroinflammation via the HMGB1/TLR4/NF-κB network[J].J Cell Mol Med,2023,27(12):1653-1663.
[11] 王凯华,杨鑫勇,李岩,等.柚皮素通过调控细胞增殖和线粒体自噬发挥神经细胞保护作用实验研究[J].陕西医学杂志,2023,52(7):779-782.
[12] GLICK D,BARTH S,MACLEOD K F.Autophagy:Cellular and molecular mechanisms[J].J Pathol,2010,221(1):3-12.
[13] TANIDA I,UENO T,KOMINAMI E.LC3 and autophagy[J].Methods Mol Biol,2008,445:77-88.
[14] 胡跃强,陈炜,祝美珍,等.清热化瘀方通过调控自噬相关基因P62/LC3保护大鼠脑缺血再灌注损伤[J].陕西中医,2020,41(4):429-433.
[15] 杜静,贾冬雪,王书华.荭草苷调控PI3K/Akt通路对脑缺血再灌注大鼠脑组织线粒体自噬的影响[J].河北医药,2022,44(7):1034-1037.
[16] 黄钢,马善波,杨双武,等.鹅不食草介导Nrf2/HO-1信号通路在脑缺血再灌注中抗氧化抗炎作用机制研究[J].陕西中医,2020,41(12):1699-1703.
[17] YU L,ZHANG Y,CHEN Q,et al.Formononetin protects against inflammation associated with cerebral ischemia-reperfusion injury in rats by targeting the JAK2/STAT3 signaling pathway[J].Biomed Pharmacother,2022,149:112836.
[18] SONG Y Q,ZOU H L,ZHAO Y J,et al.Activation of p38-mitogen-activated protein kinase contributes to ischemia reperfusion in rat brain[J].Genet Mol Res,2016,15(3):1-11.
[19] COULTHARD L R,WHITE D E,JONES D L,et al.p38(MAPK):Stress responses from molecular mechanisms to therapeutics[J].Trends Mol Med,2009,15(8):369-379.
[20] HOU K,XIAO Z C,DAI H L.p38 MAPK endogenous inhibition improves neurological deficits in global cerebral ischemia/reperfusion mice[J].Neural Plast,2022,2022:3300327.
[21] 张奎明,葛鸾蝶,张林悦,等.基于p38MAPK信号通路探讨中医药防治脑缺血再灌注损伤[J].广州中医药大学学报,2022,39(6):1417-1422.
[22] 高晶晶.化浊解毒活血通络法对大鼠脑缺血再灌注损伤后神经细胞凋亡及P38MAPK信号通路的影响[D].石家庄:河北医科大学,2016.
[23] DUAN X,SONG N,MA K,et al.The effects of protein-rich extract from Rhizoma Gastrodiae against cerebral ischemia/reperfusion injury via regulating MAPK and PI3K/AKT signaling pathway[J].Brain Res Bull,2023,203:110772.
[24] PAN B,SUN J,LIU Z,et al.Longxuetongluo capsule protects against cerebral ischemia/reperfusion injury through endoplasmic reticulum stress and MAPK-mediated mechanisms[J].J Adv Res,2021,33:215-225.
[25] HUANG L,CHEN C,ZHANG X,et al.Neuroprotective effect of curcumin against cerebral ischemia-reperfusion via mediating autophagy and inflammation[J].J Mol Neurosci,2018,64(1):129-139.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82360870); 广西自然科学基金资助项目(2021GXNSFAA196012,2023GXNSFAA026404); 广西重点研发计划项目(桂科AB24010222); 广西壮族自治区中医药管理局自筹科研项目(GXZYZ20210103); 广西中医药大学科研项目(2021MS011)
更新日期/Last Update: 2024-12-05