[1]宋佳明,张德生.牛膝总皂苷抑制椎间盘纤维环细胞焦亡的Wnt/β-catenin通路机制[J].陕西中医,2025,46(3):325-329.[doi:DOI:10.3969/j.issn.1000-7369.2025.03.008]
　SONG Jiaming,ZHANG Desheng.Mechanism of inhibiting intervertebral disc annulus fibrosus cell pyroptosis by achyranthes bidentata total saponins through regulating Wnt/β-catenin pathway[J].,2025,46(3):325-329.[doi:DOI:10.3969/j.issn.1000-7369.2025.03.008]
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牛膝总皂苷抑制椎间盘纤维环细胞焦亡的Wnt/β-catenin通路机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年3期
页码:
325-329
栏目:
基础研究
出版日期:
2025-03-05

文章信息/Info

Title:
Mechanism of inhibiting intervertebral disc annulus fibrosus cell pyroptosis by achyranthes bidentata total saponins through regulating Wnt/β-catenin pathway
作者:
宋佳明; 张德生
(湖南中医药大学第二附属医院,湖南 长沙 410005)
Author(s):
SONG Jiaming; ZHANG Desheng
(The Second Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410005,China)
关键词:
牛膝总皂苷;  Wnt/β-catenin;  信号通路;  椎间盘纤维环细胞;  细胞焦亡;  半胱氨酸蛋白酶-1;  白细胞介素-18;  白细胞介素-1β
Keywords:
Total saponin;  Wnt/β-catenin;  Intervertebral disc fibrocytes;  Cell death;  Cysteine protease-1;  IL-18;  IL-1β
分类号:
R 28
DOI:
DOI:10.3969/j.issn.1000-7369.2025.03.008
文献标志码:
A
摘要:
目的:基于Wnt/β-catenin通路探讨牛膝总皂苷对抑制椎间盘纤维环细胞焦亡的影响。方法:将105只SPF级SD大鼠随机分为牛膝总皂苷低、中、高剂量组,模型组,正常对照组,假手术组,阻断剂组,每组15只。除正常对照组外,其余各组均进行大鼠腰椎间盘退变模型造模,假手术组仅暴露椎间盘后缝合,不造成退变。术后3 d,牛膝总皂苷组分别按60、120、240 μg/g剂量灌胃给药; 阻断剂组皮下注射Wnt/β-catenin通路抑制剂; 正常对照组、假手术组、模型组则给予等量0.9%氯化钠溶液灌胃。连续干预2周后,采用Western blot和qPCR技术检测各组椎间盘纤维环中Caspase-1、IL-18、IL-1β的蛋白和mRNA表达水平。结果:qPCR检测结果显示,与正常对照组、假手术组相比,模型组可见Caspase-1、IL-1β、IL-18 mRNA表达显著上调,经牛膝总皂苷药物干预后,与模型组相比较,牛膝总皂苷组Caspase-1、IL-1β、IL-18 mRNA表达水平显著降低,差异有统计学意义(均P<0.01)。经阻断剂药物干预后,与模型组相比较,Caspase-1、IL-1β、IL-18 mRNA表达显著降低(均P<0.01)。Western blot检测结果显示,与正常对照组、假手术组对照相比,模型组可见Caspase-1、IL-1β、IL-18蛋白表达水平上调,经牛膝总皂苷药物干预后,与模型组比较,牛膝总皂苷组Caspase-1、IL-1β、IL-18蛋白表达降低,差异有统计学意义(均P<0.01)。经阻断剂药物干预后,与模型组相比较,Caspase-1、IL-1β、IL-18蛋白表达显著降低(均P<0.01)。结论:牛膝总皂苷抑制椎间盘纤维环细胞焦亡的机制可能与调控Wnt/β-catenin信号通路有关。
Abstract:
Objective:To investigate effect of total saponins of achyranin on the inhibition of focal death of intervertebral disc fibroring cells based on Wnt/β-catenin pathway.Methods:105 SPF-grade SD rats were randomly divided into low,medium,and high dose groups of total saponin of hyssop,model group,normal control group,sham-operation group,and blocker group,with 15 rats in each group.Except the normal control group underwent modelling of lumbar disc degeneration model in rats,and the sham-operation group only exposed the disc and then sutured it without causing degeneration.Starting from 3 days after surgery,the total saponin of hyssop group was administered by gavage at the doses of 60 μg/g,120 μg/g,240 μg/g,respectively.The blocker group were injected subcutaneously with Wnt/β-catenin pathway inhibitor,and equal amounts of saline were given by gavage to the normal control group,the sham-operated group,and the modelling group.After 2 weeks of continuous intervention,the protein and mRNA expression levels of Caspase-1,IL-18,and IL-1β in the intervertebral disc fibrous annulus of each group were detected by Western blot and qPCR.Results:The results of qPCR assay showed that expression levels of Caspase-1,IL-1β and IL-18 mRNA were significantly up-regulated in the model group compared with the control of normal group and sham operation group(all P<0.01).After the drug intervention of total saponin of cow's knee,the expression levels of Caspase-1,IL-1β and IL-18 mRNA in the group of total saponin of cow's knee,compared with that in the model group,difference statistically significant(all P<0.01).After the blocker drug intervention,compared with the model group,expression levels of Caspase-1,IL-1β and IL-18 mRNA were significantly reduced(all P<0.01).The results of Western blot showed that,compared with the normal group and the sham-operated group control,the model group showed significant up-regulation of the expression levels of Caspase-1,IL-1β and IL-18 proteins,and protein expression levels were significantly up-regulated(all P<0.01).After drug intervention with total saponin of cow's knee,the protein expression levels of Caspase-1,IL-1β and IL-18 in the total saponin of cow's knee group were significantly reduced compared with those in the model group(all P<0.01).After drug intervention with a blocker,compared with those in the model group,protein expression levels of Caspase-1,IL-1β and IL-18 were were significantly reduced(all P<0.01).Conclusion:Mechanism of total saponins of hyssop inhibiting the cellular death of intervertebral disc fibrous annulus may be related to regulation of Wnt/β-catenin signalling pathway.

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备注/Memo

备注/Memo:
[基金项目]湖南省卫生健康委员会科研课题(D202304077262); 湖南省中药管理局重点课题(A2024037)
更新日期/Last Update: 2025-03-10