[1]赖菁菁,向军军,韩海涛,等.壮医莲花针拔罐逐瘀法对带状疱疹后神经痛小鼠Wnt3a/β-catenin通路的影响[J].陕西中医,2025,46(4):435-439.[doi:DOI:10.3969/j.issn.1000-7369.2025.04.001]
　LAI Jingjing,XIANG Junjun,HAN Haitao,et al.Effect of Zhuang medicine lotus acupuncture cupping stasis therapy on Wnt3a/β-catenin pathway in mice with post herpetic neuralgia[J].,2025,46(4):435-439.[doi:DOI:10.3969/j.issn.1000-7369.2025.04.001]
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壮医莲花针拔罐逐瘀法对带状疱疹后神经痛小鼠Wnt3a/β-catenin通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年4期
页码:
435-439
栏目:
基础研究
出版日期:
2025-04-20

文章信息/Info

Title:
Effect of Zhuang medicine lotus acupuncture cupping stasis therapy on Wnt3a/β-catenin pathway in mice with post herpetic neuralgia
作者:
赖菁菁; 向军军; 韩海涛; 李 婕; 黄瑾明; 陈秋霞; 李秀娟; 李伟茜; 李美康
(广西中医药大学第一附属医院,广西 南宁 530023)
Author(s):
LAI Jingjing; XIANG Junjun; HAN Haitao; LI Jie; HUANG Jinming; CHEN Qiuxia; LI Xiujuan; LI Weiqian; LI Meikang
(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)
关键词:
带状疱疹后神经痛;  壮医莲花针拔罐逐瘀法;  Axis抑制蛋白2;  N-甲基-D-天冬氨酸受体2B;  神经激肽1;  Wnt3a/β-catenin通路
Keywords:
Post herpetic neuralgia;  Zhuang medicine lotus acupuncture cupping stasis therapy;  Axin2;  NR2B;  NK-1;  Wnt3a/β-catenin Pathway
分类号:
R 752.1
DOI:
DOI:10.3969/j.issn.1000-7369.2025.04.001
文献标志码:
A
摘要:
目的:探讨壮医莲花针拔罐逐瘀法对带状疱疹后神经痛(PHN)小鼠行为学变化的影响及其对Wnt3a/β-catenin通路效应机制。方法:40只KM小鼠随机分为对照组、模型组、加巴喷丁组、壮医莲花针拔罐逐瘀组(壮医组),每组10只。采用树脂毒素(RTX)制作PHN小鼠模型,观察干预后各组小鼠行为学机械痛觉阈值(PWT)、热痛觉阈值(TWL)的变化; ELISA检测N-甲基-D-天冬氨酸受体2B亚单(NR2B)、神经激肽-1(NK-1)含量; 免疫组化及WB检测Wnt3a、Axin2因子表达。结果:造模后,与对照组相比,模型组PWT降低,TWL升高,提示模型构建成功; 与模型组相比,加巴喷丁组和壮医组分别在第3、4节点行为学测试PWT显著升高,TWL显著降低(均P<0.05); 与加巴喷丁组相比,壮医组在第6次节点PWT显著升高,TWL显著降低(均P<0.05)。与对照组相比,PHN组NR2B、NK-1表达显著升高(均P<0.05); 与模型组相比,加巴喷丁组和壮医组NR2B、NK-1表达明显降低(均P<0.05)。与对照组相比,模型组Wnt3a表达升高,Axin2表达降低(均P<0.05); 与模型组相比,加巴喷丁组与壮医组Wnt3a表达显著降低,Axin2表达升高(均P<0.05)。结论:壮医莲花针拔罐逐瘀法治疗PHN可能通过抑制或阻断Wnt3a/β-catenin通路,抑制神经递质释放,从而减轻痛觉过敏发挥镇痛作用。
Abstract:
Objective:To observe the effect of Zhuang medicine lotus acupuncture cupping stasis therapy on the behavior of mice with post herpetic neuralgia and its mechanism on Wnt3a/β-catenin pathway.Methods:Forty mice were randomly divided into control group,post herpetic neuralgia(PHN)group,positive(JBPD)group and Zhuang medicine lotus acupuncture(ZY)group,with 10 mice in each group.The PHN model was prepared by intraperitoneal injection of resin toxin and then observe the mechanical foot withdrawal threshold(PWT)and thermal pain threshold(TWL)of each group.The contents of NR2B and NK-1 were detected by ELISA,and the expressions of Wnt3a and Axin2 were detected by Western blot and immunohistochemistry.Results:Compared with control group,PWT decreased and TWL increased after modeling.Compared the PHN group,the PWT of the JBPD group and the ZY group increased significantly and the TWL decreased significantly in the 3rd and 4th node behavioral tests(all P<0.05).Compared with the JBPD group,the PWT of the ZY group increased significantly and TWL decreased significantly at the 6th node(all P<0.05).Compared with control group,the expression of NR2B and NK-1 in PHN Group was significantly increased(all P<0.05),while the expression of NR2B and NK-1 in JBPD group and ZY group was significantly decreased compared with PHN group(all P<0.05).Compared with control group,Wnt3a expression was increased and Axin2 expression was decreased in PHN group(all P<0.05).Compared with PHN group,Wnt3a expression in JBPD group and ZY group was significantly decreased,and Axin2 expression was increased(all P<0.05).Conclusion:The treatment of PHN with Zhuang medicine lotus acupuncture cupping stasis therapy may inhibit or block the Wnt3a/β-catenin pathway,inhibit the release of neurotransmitters,and thus relieve hyperalgesia and play an analgesic role.

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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金资助项目(82060904); 广西壮族自治区中医药管理局壮医学重点学科项目(GZXK-Z-20-59); 广西黄氏壮医针灸流派传承工作室建设项目[国中医药人教函(2019)62号]; 广西名中医李美康传承工作室建设项目[桂中医药科教发(2024)1号]
更新日期/Last Update: 2025-04-08