[1]罗 斌,陈雨燕,邱秀芳,等.清金化痰汤抑制IL-6/STAT3/HIF-1α通路介导的细胞凋亡和炎症治疗慢性阻塞性肺疾病机制研究[J].陕西中医,2025,46(4):462-466.[doi:DOI:10.3969/j.issn.1000-7369.2025.04.006]
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清金化痰汤抑制IL-6/STAT3/HIF-1α通路介导的细胞凋亡和炎症治疗慢性阻塞性肺疾病机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年4期
页码:
462-466
栏目:
基础研究
出版日期:
2025-04-20

文章信息/Info

作者:
罗 斌; 陈雨燕; 邱秀芳; 魏小杰; 王燕娇; 朱烨芳
(福建中医药大学附属第三人民医院,福建 福州 350108)
关键词:
慢性阻塞性肺疾病;  清金化痰汤;  IL-6/STAT3/HIF-1α通路;  细胞凋亡;  炎症;  地塞米松
分类号:
R 563
DOI:
DOI:10.3969/j.issn.1000-7369.2025.04.006
文献标志码:
A
摘要:
目的:探讨清金化痰汤(QJHTD)抑制白介素-6(IL-6)/信号转导与转录激活因子3(STAT3)/低氧诱导因子1α(HIF-1α)通路介导的细胞凋亡和炎症治疗慢性阻塞性肺疾病(COPD)的机制。方法:采用烟熏联合气管内注射脂多糖的方法建立大鼠COPD模型。将50只SPF级SD雄性大鼠随机分为对照组(CON组)、模型组(COPD组)、清金化痰汤低剂量组(QJHTD-L组)、清金化痰汤高剂量组(QJHTD-H组)以及地塞米松组(DXMS组),每组10只。给药结束后,观察各组大鼠的生存状态; HE染色检测大鼠肺组织的病理变化以及支气管肺泡灌洗液(BALF)中炎症细胞的数量; TUNEL法检测大鼠的肺上皮细胞凋亡情况; ELISA试剂盒检测大鼠BALF中肿瘤坏死因子-α(TNF-α)、IL-6、白介素-1β(IL-1β)的含量; WB检测大鼠BALF中IL-6、STAT3、HIF-1α的蛋白相对表达量。结果:与CON组相比,COPD组大鼠呼吸困难、精神萎靡、皮毛发黄; 肺组织病理显示肺泡部分融合,有大量炎性细胞浸润; 肺上皮细胞凋亡细胞指数显著增加(P<0.05); BALF中的中性粒细胞、巨噬细胞、淋巴细胞数量显著增加(均P<0.05),TNF-α、IL-6、IL-1β水平明显升高(均P<0.05),IL-6、STAT3、HIF-1α蛋白相对表达量显著增加(均P<0.05)。与COPD组相比,QJHTD-H和DXMS组大鼠表观症状明显缓解; QJHTD-H和DXMS组的中性粒细胞、淋巴细胞数量显著减少(均P<0.05),QJHTD-L、QJHTD-H和DXMS组的巨噬细胞数量显著减少(均P<0.05),TNF-α、IL-6、IL-1β水平明显下降(均P<0.05),IL-6、STAT3、HIF-1α的蛋白相对表达量显著减少(均P<0.05)。QJHTD-H组和DXMS组的治疗效果更接近。结论:清金化痰汤能够通过抑制IL-6/STAT3/HIF-1α通路介导的细胞凋亡和炎症治疗COPD。

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备注/Memo

备注/Memo:
[基金项目]福建省卫健委科技计划项目(2020QNA066)
更新日期/Last Update: 2025-04-08