[1]王益东,张 薇,徐 剡,等.胎肾细胞悬液对去卵巢大鼠促性腺激素释放激素受体影响的实验研究[J].陕西中医,2025,46(5):585-588,610.[doi:DOI:10.3969/j.issn.1000-7369.2025.05.002]
　WANG Yidong,ZHANG Wei,XU Yan,et al.Experimental study on the effects of fetal renal cell suspension on Gonadotropin-releasing hormone receptor(GnRHR)in ovariectomized rats[J].,2025,46(5):585-588,610.[doi:DOI:10.3969/j.issn.1000-7369.2025.05.002]
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胎肾细胞悬液对去卵巢大鼠促性腺激素释放激素受体影响的实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年5期
页码:
585-588,610
栏目:
基础研究
出版日期:
2025-05-05

文章信息/Info

Title:
Experimental study on the effects of fetal renal cell suspension on Gonadotropin-releasing hormone receptor(GnRHR)in ovariectomized rats
作者:
王益东1; 张 薇2; 徐 剡2; 刘 昳3; 郑清莲3; 边卓琼2
(1.西安交通大学第二附属医院,陕西 西安 710004; 2.西安市第五医院,陕西 西安 710082; 3.西安交通大学第一附属医院,陕西 西安 710061)
Author(s):
WANG Yidong1; ZHANG Wei2; XU Yan2; LIU Yi3; ZHENG Qinglian3; BIAN Zhuoqiong2
(1.Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China; 2.Fifth Hospital of Xi'an,Xi'an 710082,China; 3.First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)
关键词:
胎肾细胞悬液;  去卵巢大鼠;  下丘脑-垂体-卵巢轴;  GnRHR;  雌激素;  肾主生殖
Keywords:
Fetal renal cell suspension;  Ovariectomized rat;  Hypothalamic-pituitary-ovarian axis;  GnRHR;  Estrogen;  Kidney dominates reproduction
分类号:
R 271.116
DOI:
DOI:10.3969/j.issn.1000-7369.2025.05.002
文献标志码:
A
摘要:
目的:探讨大鼠去卵巢后促性腺激素释放激素受体(GnRHR)的变化及胎肾细胞悬液(FRCS)对其干预机制。方法:取3月龄未交配健康SD雌性大鼠64只,随机分为四组,即假手术组、模型组、雌激素组、胎肾组。其中假手术组行假手术,模型组、雌激素组、胎肾组行去卵巢手术。术后8周,假手术组、模型组给予0.9%氯化钠溶液,雌激素组给予苯甲酸雌二醇,胎肾组给予胎肾细胞悬液。药物治疗4周后,利用免疫组织化学、RT-PCR检测GnRHR在下丘脑的表达。结果:与假手术组相比,模型组GnRHR表达量明显升高(均P<0.01); 与模型组相比,雌激素组、胎肾组GnRHR表达量降低(P<0.05); 干预两组间两两比较,差异均无统计学意义(P>0.05)。结论:去卵巢后大鼠GnRHR表达量增高,应用胎肾细胞悬液进行干预,能降低GnRHR表达量,增加机体的雌激素水平,延缓机体绝经年龄。
Abstract:
Objective:To observe the change of Gonadotropin releasing hormone receptor(GnRHR)in rats after ovariectomy and the intervention effect of fetal renal cell suspension(FRCS)on them.Methods:Sixty-four 3-month-old unmated healthy SD female rats were taken and randomly divided into four groups:normal group,model group,estrogen group,and fetal renal cell suspension group.Eight weeks after operation,the rats in normal group and model group were treaded with sodium chloride,the estrogen group was treated with estrogen,the fetal renal cell suspension group was treated with FNCS.All of the rats were sacrificed after four weeks.Immunohistochemistry and RT-PCR methods were used to observer the changes in the expression of GnRHR in hypothalamus.Results:GnRHR levels in model group were substantially higher than in normal group(P<0.01).GnRHR levels in estrogen group and fetal renal cell suspension group were considerably lower than in model group(P<0.05),although there was no significant difference between the two groups in paired comparisons(P>0.05).Conclusion:Rats with ovariectomies undoubtedly have high levels of free radicals; FRCS can reduce these damage and improve oxidation-antagonistic effects.

参考文献/References:

[1] 周美清.现代老年妇科学[M].北京:人民军医出版社,1999:48-56.
[2] 王志红,洪领俊,王菊荣.理气散结方联合米非司酮治疗气滞血瘀证子宫肌瘤的临床疗效及对性激素水平、血液流变学指标的影响[J].中国性科学,2024,33(4):144-147.
[3] DUBOIS E A,ZANDBERGEN M A,PEUTE J,et al.Evolutionary development of three GnRH systems in vertebrates[J].Brain Research Bulletin,2002,57(34):413-418.
[4] GORBMAN A,SOWER S A.Evolution of the role of GnRH in animal(Metazoan)biology[J].Gen Comp Endocrinal,2003,134(3):207-213.
[5] 李佳.中西医结合治疗围绝经期综合征的临床疗效[J].保健医学研究与实践,2018,15(2):47-50.
[6] 刘良春,胡春芹,徐敏,等.湖北省随州市城区围绝经期妇女对女性激素的知晓及应用调查分析[J].深圳中西医结合杂志,2016,26(10):179-180.
[7] 李秀芳,王忠清,朱蕾,等.补肾中药改善高龄不孕女性卵母细胞氧化应激损伤的研究进展[J].世界中医药,2022,17(22):3263-3269.
[8] 李岚,景静源.中药穴位贴敷治疗肾虚型先兆流产的疗效观察及其对性激素水平、子宫动脉血流动力学的影响[J].中国中医药科技,2024,31(4):631-633.
[9] 周婷婷,郝宁.补肾活血中药联合维生素D对PCOS不孕患者内分泌及抗氧化作用的影响[J].湖南中医杂志,2021,37(6):54-57.
[10] 蔡艳,张晓丹,孙琦玉,等.补肾填精方对围绝经期综合征患者血清性激素水平及子宫内膜厚度的影响[J].长春中医药大学学报,2023,39(3):306-309.
[11] 赵昱璇,梁启惠,陈雯,等.基于“肾主生殖”理论探讨中医治疗DOR型不孕症的生物学机制[J].湖南中医药大学学报,2024,44(2):320-326.
[12] SHIMIZU T,KAMEGAI J.TAMURA H,et al.The estrogen receptor(ER)alpha,but not ER beta,gene is expressed in hypothalamic growth hormone-releasing hormone neurons of the adult female rat[J].Neurosci Res,2005,52(1):121-125.
[13] CHILDS G V,IRUTHAYANATHAN M,AKHTER N,et al.Bipotential effects of estrogen on growth hormone synthesis and storage in vitro[J].Endocrinology,2005,146(4):1780-1788.
[14] 王金鸾,张宁,郭颖,等.补肾中药对高龄肾气虚不孕症患者胚胎发育时间参数的影响[J].时珍国医国药,2023,34(4):898-901.
[15] 陈志雯.中药补肾调冲方对雷公藤致大鼠卵巢早衰的治疗及机制研究[D].承德:承德医学院,2024.
[16] 王如烨,章勤,方晓红.何氏育麟方治疗卵巢储备功能下降作用机制的网络药理学和分子对接研究[J].浙江中西医结合杂志,2024,34(1):64-70.
[17] 曲淑艳,章勤.何氏育麟方联合音乐疗法治疗卵巢储备功能下降的疗效观察及其对生殖激素及卵巢储备功能、血流的影响[J].中国中医药科技,2023,30(5):895-897.
[18] 周狄,李杰,陈伶利,等.加味左归饮联合隔姜灸治疗肾阴虚眩晕临床观察[J].湖北中医杂志,2023,45(5):40-42.
[19] 梁洁源,张帆,王文娜,等.加味左归饮治疗粤西地区肾阴虚型更年期综合征的疗效观察[J].广州中医药大学学报,2021,38(4):717-722.
[20] 张蓝予,陈涛,梁珊珊.更年期综合征运动干预研究综述[J].辽宁体育科技,2016,38(4):50-53.
[21] 潘丽,唐文龙,贠小巧,等.中医药针灸疗法治疗更年期综合征的研究进展[J].内蒙古中医药,2023,42(1):155-158.
[22] 郑清莲,赵欣,彭宁,等.胎肾细胞悬液对老龄大鼠骨质的影响[J].中国老年学杂志,2002,2(2):146-147.
[23] FANIS P,NEOCLEOUS V,PAPAPETROU I,et al.Gonadotropin-releasing hormone receptor(GnRHR)and hypogonadotropic hypogonadism [J]. Int J Mol Sci,2023,24(21):159-165.
[24] 谢凤云,谢梅青.GnRH受体在人类子宫内膜的表达及意义[J].海南医学,2012,23(7):114-116.
[25] 陆炜.补肾中药对下丘脑-垂体-性腺轴相关受体的调节作用[D].上海:复旦大学,2002.

备注/Memo

备注/Memo:
[基金项目]国家自然科学基金青年基金资助项目(81602294); 郑清莲全国名老中医药专家传承工作室建设项目
更新日期/Last Update: 2025-05-08