[1]盛紫阳,栾仲秋,邓升华.苁蓉复肾方在抑制单侧输尿管梗阻大鼠肾间质纤维化中的应用及作用研究[J].陕西中医,2025,46(5):594-599.[doi:DOI:10.3969/j.issn.1000-7369.2025.05.004]
　SHENG Ziyang,LUAN Zhongqiu,DENG Shenghua.Application and effect of Congrong Fushen prescription on inhibiting renal interstitial fibrosis in rats with unilateral ureteral obstruction[J].,2025,46(5):594-599.[doi:DOI:10.3969/j.issn.1000-7369.2025.05.004]
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苁蓉复肾方在抑制单侧输尿管梗阻大鼠肾间质纤维化中的应用及作用研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年5期
页码:
594-599
栏目:
基础研究
出版日期:
2025-05-05

文章信息/Info

Title:
Application and effect of Congrong Fushen prescription on inhibiting renal interstitial fibrosis in rats with unilateral ureteral obstruction
作者:
盛紫阳1; 2; 3; 栾仲秋1; 2; 邓升华1; 2
(1.黑龙江中医药大学,黑龙江 哈尔滨 150040; 2.黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040; 3.哈尔滨医科大学,黑龙江 哈尔滨 150081)
Author(s):
SHENG Ziyang1; 2; 3; LUAN Zhongqiu1; 2; DENG Shenghua1; 2
(1.Heilongjiang University of Chinese Medicine,Harbin 150040,China; 2.The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China; 3.Harbin Medical University,Harbin 150081,China)
关键词:
肾间质纤维化;  苁蓉复肾方;  肾组织病理涂片;  免疫组化;  慢性肾脏病;  输尿管梗阻
Keywords:
Renal interstitial fibrosis;  Congrong Fushen prescription;  Renal tissue pathology smear;  Immunohistochemistry;  Chronic kidney disease;  Ureteral obstruction
分类号:
R 692
DOI:
DOI:10.3969/j.issn.1000-7369.2025.05.004
文献标志码:
A
摘要:
目的:本研究以实验为基础,通过建立单侧输尿管梗阻(UUO)大鼠模型,对其形态学的影响及肾间质组织功能进行研究,并利用免疫组化实验方法,对其发生机制、抗肾间质纤维化的作用进行探讨。方法:本实验选取研究对象为60只SPF级健康雄性SD大鼠,随机分为手术组45只,假手术组15只,手术组大鼠在做完UUO模型后,再随机分成三组,模型组、苁蓉复肾方组、厄贝沙坦组,每组各15只。药液灌胃14 d后,各组随机选取10只大鼠,分别采集24 h尿液、血清和大鼠梗阻侧肾组织,用于测定血清肌酐、尿素氮、Ang-Ⅱ蛋白、MCP-1蛋白以及24 h尿蛋白等,其中梗阻侧肾组织一部分用于病理观察,另一部分用于免疫检测。结果:在苁蓉复肾方中的测定数值中,各剂量药组大鼠的血清肌酐、尿素氮比模型组大鼠明显降低。苁蓉复肾方组大鼠肾脏组织发生的病理变化,比模型组大鼠程度低。苁蓉复肾方组大鼠血清中Ang-Ⅱ蛋白的含量较低。大鼠血清中MCP-1蛋白在苁蓉复肾方组中含量较高。苁蓉复肾方组大鼠体内TNF-α蛋白与模型组相比表达量较低。苁蓉复肾方组大鼠中ColⅣ蛋白的表达低于模型组。结论:苁蓉复肾方可使血肌酐、尿素氮、24 h尿蛋白定量降低,减轻单侧输尿管梗阻引起的肾脏损伤。降低UUO模型大鼠的肾脏病理损害,促进其血清中MCP-1蛋白的表达,进而抑制血清中的Ang-Ⅱ蛋白、TNF-α蛋白,以达到降低ColⅣ的蛋白含量,减少ECM的合成和积累的目的,缓解肾间质纤维化进程。苁蓉复肾方在一定程度上保护肾功能,使肾小管损伤程度减轻,肾间质纤维化程度降低,为肾间质纤维化的临床治疗提供依据。
Abstract:
Objective:In this study,a rat model of unilateral ureteral obstruction(UUO)was established based on the experimental design to investigate its morphological effects and renal interstitial tissue function.Immunohistochemical methods were employed to explore the pathogenesis and mechanisms underlying anti-renal interstitial fibrosis.Methods:In this experiment,60 healthy male SD white rats of SPF grade were selected as research subjects,and they were randomly divided into surgery group(45),sham surgery group(15),and the surgery group rats were divided into three groups after the UUO model was made,namely,model group,Astragalus membranaceus Congrong Fushen prescription group,and Irbesartan group,with 15 rats in each group.After 14 days of oral administration of the solution,10 rats were randomly selected from each group to collect 24-hour urine,serum,and the obstructed kidney tissue of the rats for the determination of serum creatinine,urea nitrogen,Ang-Ⅱ protein,MCP-1 protein,and 24-hour urine protein,and part of the obstructed kidney tissue was used for pathological observation,and the other part was used for immune detection.Results:The Congrong Fushen prescription group exhibited significantly lower levels of serum creatinine and urea nitrogen compared to the model group.The pathological changes in the renal tissue of the Congrong Fushen prescription group were less severe than those in the model group.The Congrong Fushen prescription group also showed lower levels of serum Ang-Ⅱ protein and higher levels of serum MCP-1 protein.Additionally,the expression of TNF-α protein in the Congrong Fushen prescription group was lower compared to the model group.The expression of Col Ⅳ protein in the Congrong Fushen prescription group was also lower than that in the model group.Conclusion:Congrong Fushen prescription has a protective effect on renal injury induced by unilateral ureteral obstruction(UUO),reducing blood creatinine,urea nitrogen,and 24-hour urinary protein levels.It alleviates renal pathological damage in UUO model rats,promotes the expression of MCP-1 protein in the serum of these rats,and inhibits the expression of Ang-Ⅱ and TNF-α proteins in the serum.Consequently,it decreases the protein content of ColⅣ,reduces the synthesis and accumulation of extracellular matrix(ECM),and slows down the progression of renal interstitial fibrosis.To a certain extent,Congrong Fushen prescription protects renal function,mitigating the degree of tubular damage and reducing the extent of renal interstitial fibrosis,thereby providing a basis for the clinical treatment of renal interstitial fibrosis.

参考文献/References:

[1] 刘明林,封霞,郑杨,等.HIF-1α与慢性肾脏病患者认知功能障碍的相关性研究[J].保健医学研究与实践,2024,21(1):145-149,156.
[2] 白雪莲,张佳宜,项国梁,等.中国成人慢性肾脏病患病率的Meta分析[J].中国医药科学,2022,12(9):49-53.
[3] 刘慧芳,何泽云.怡肾丸对单侧输尿管结扎模型大鼠LC3及Beclin-1表达的影响[J].亚太传统医药,2024,20(4):10-15.
[4] 王金荣,熊洋洋,李斌超,等.肾纤维化的研究进展[J].医学研究杂志,2015,44(6):158-160.
[5] 曾聪聪,俞文秀,王成功,等.新肾病1号方调控miR-199b-5p抑制肾小管上皮间充质转化改善肾纤维化的作用研究[J].中草药,2024,55(5):1600-1608.
[6] 罗玉芳,李怀玉,吕森浩,等.肾衰泄浊汤对腺嘌呤致肾阳虚型慢性肾衰竭大鼠下丘脑-垂体-肾上腺轴的影响[J].陕西中医,2024,45(9):1161-1165.
[7] 黎艳,刘莉,王琪文,等.槲皮素对小鼠单侧输尿管结扎致肾间质纤维化的改善作用及机制研究[J].陕西医学杂志,2023,52(11):1473-1482.
[8] 张红梅,曹旭,王东.肾间质纤维化实验动物模型的多角度筛选[J].贵阳中医学院学报,2016,38(3):13-18.
[9] 陈志红,卢春键,易无庸.中医药治疗慢性肾功能衰竭临床进展[J].新中医,2018,50(3):26-29.
[10] 朱军,赵希敏,刘勇.肉苁蓉总苷对缺血再灌注肾损伤保护作用的实验研究[J].世界中西医结合杂志,2015,10(1):19-21.
[11] 吴振.肉苁蓉苷-A对小鼠缺血再灌注肾损伤防治作用的研究[D].蚌埠:蚌埠医学院,2022.
[12] 陈曦,董凯旋,周荣,等.中药调控p38丝裂原活化蛋白激酶/核因子κB通路治疗肾纤维化研究进展[J].陕西中医,2023,44(7):980-986.
[13] 陈廷芳,郭永平,桂定坤,等.黄芪甲苷干预高糖诱导的足细胞转分化的体外研究[J].中国中西医结合肾病杂志,2017,18(6):482-485.
[14] 夏文彬,石阿茜,沈阗阗,等.女贞子-淫羊藿药对对顺铂所致大鼠肾毒性的保护作用[J].西南医科大学学报,2023,46(6):524-529.
[15] 林婷婷,李小翠,邱华伟,等.淫羊藿低糖苷组分安全性评价及其5种主要成分的药代动力学研究[J].中药新药与临床药理,2024,53(3):402-410.
[16] BI Z Y,ZHANG W,YAN X Y.Anti-inflammatory and immunoregulatory effects of icariin and icaritin [J].Biomed Pharmacother,2022,151:113180.
[17] 李曼曼,刘永.炮制方法对女贞子中红景天苷和酪醇含量的影响[J].中西医结合研究,2020,12(3):165-167.
[18] 杨杰,魏文一,景彩芳,等.基于网络药理学探讨女贞子治疗糖尿病肾病作用机制[J].中国中医药现代远程教育,2024,22(5):126-129.
[19] 谷妮妮,樊娟,朱晶惠,等.贞芪扶正颗粒在不同疾病治疗中的作用研究进展[J].陕西医学杂志,2024,53(5):707-713.
[20] 夏文彬.女贞子及其复方制剂对化疗药顺铂致肾损伤的保护作用研究[D].兰州:兰州大学,2022.
[21] MEI M X,CAO Y,CHE Y,et al.Danshen:A phytochemical and pharmacological overview[J].Chinese Journal of Natural Medicines,2019,17(1):59-80.
[22] 张舒,苏保林,王亮亮,等.丹参酮ⅡA通过抑制RIP3/FUNDC1信号通路减轻LPS诱导的小鼠肾小管上皮细胞凋亡[J].南方医科大学学报,2022,42(12):1852-1857.
[23] 田江明,李均.丹参及其单体成分防治肾纤维化的机制研究进展[J].中医药导报,2023,29(5):204-207.
[24] 龙佳瑜,范崇峰.“落得打”本草考证[J].河南中医,2023,43(12):1814-1820.
[25] 张敏,谢圣芳,胡泳,等.积雪草在肾脏病领域的临床应用和机制探讨[J].中国中西医结合肾病杂志,2021,22(4):375-376.
[26] 杨春兰,陈洪宇,王曾.中药药对治疗慢性肾衰竭的研究进展[J].中国现代医生,2023,61(36):121-124.
[27] 朱恒杰,曾允富,程少文,等.积雪草苷通过Nrf2/HO-1信号通路对创伤脓毒血症大鼠肾损伤的保护作用研究[J].湖南中医药大学学报,2023,43(1):14-20.
[28] 唐安琪,蒋廷,陆红梅,等.积雪草治疗慢性肾脏病研究进展[J].中药药理与临床,2022,38(5):1-14.

备注/Memo

备注/Memo:
[基金项目]黑龙江省自然科学基金资助项目(LH2022H073); 黑龙江省中医药管理局项目(ZHY2020-121); 宋立群全国名老中医专家传承工作室建设项目[国中医药人教涵(2022)75 号]
更新日期/Last Update: 2025-05-08