[1]蒋著椿,彭佩纯,李小梅,等.基于HIF-1α/VEGF信号通路探讨瑶药肿瘤藤对HepG2肝癌细胞的效应机制[J].陕西中医,2025,46(6):723-728.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.001]
　JIANG Zhuchun,PENG Peichun,LI Xiaomei,et al.Exploring the effect mechanism of Yao medicine tumor vine on HepG2 hepatocellular carcinoma cells based on HIF-1α/VEGF signaling pathway[J].,2025,46(6):723-728.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.001]
点击复制

基于HIF-1α/VEGF信号通路探讨瑶药肿瘤藤对HepG2肝癌细胞的效应机制
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年6期
页码:
723-728
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
Exploring the effect mechanism of Yao medicine tumor vine on HepG2 hepatocellular carcinoma cells based on HIF-1α/VEGF signaling pathway
作者:
蒋著椿1; 彭佩纯2; 李小梅3; 李慧1; 罗彩玉1; 邓鑫1; ZSOLT Radak4(匈牙利)
（1.广西中医药大学,广西 南宁 530001；2.广西国际壮医医院,广西 南宁 530001；3.铜仁市人民医院,贵州 铜仁 554000；4.匈牙利体育与运动大学，布达佩斯 1000）
Author(s):
JIANG Zhuchun1; PENG Peichun2; LI Xiaomei3; LI Hui1; LUO Caiyu1; DENG Xin1; ZSOLT Radak4
(1.Guangxi University of Traditional Chinese Medicine,Nanning 530001,China;2.Guangxi International Zhuang Medicine Hospital,Nanning 530001,China;3.Tongren People’s Hospital,Tongren 554000,China;4.Hungarian University of Physical Education and Sport,Budapest 1000,Hungary）
关键词:
肝细胞癌; 瑶药肿瘤藤; 缺氧诱导因子-1α; 信号通路; 血管内皮生长因子; 效应机制
Keywords:
Hepatocellular carcinoma; Yao medicine tumour vine; Hypoxia inducible factor-1α; Signaling pathway; Vascular endothelial growth factor; Effect mechanism
分类号:
R 735.7
DOI:
DOI:10.3969/j.issn.1000-7369.2025.06.001
文献标志码:
A
摘要:
目的：探讨瑶药肿瘤藤通过HIF-1α/VEGF信号通路对HepG2肝癌细胞的影响，以探究其可能的效应机制。方法：体外模拟肝癌细胞缺氧微环境，制备肿瘤藤低、中、高剂量含药血清。随机分为常氧模型组、乏氧模型组、肿瘤藤低、中、高剂量组。CCK-8法检测各组细胞活性情况，采用Hoechst33342染色法检测各组细胞凋亡情况，Transwell实验检测细胞迁移能力，免疫荧光化学染色检测HIF-1α、VEGF阳性细胞表达量，流式细胞术检测细胞凋亡比率，RT-qPCR和Western blot检测各组细胞中HIF-1α、VEGF 和Caspase-1 mRNA及蛋白的表达水平。结果：与常氧模型组比较，乏氧模型组细胞活性上升，细胞凋亡比率下降，迁移细胞数增多，HIF-1α、VEGF 及Caspase-1 mRNA及蛋白表达水平升高（均P＜0.05）；与模型组相比，肿瘤藤含药血清各剂量组细胞活性降低，细胞凋亡比率上升，迁移细胞数减少，HIF-1α、VEGF及Caspase-1 mRNA及蛋白表达量下降，且中剂量组效果最佳（均P＜0.05）。结论：瑶药肿瘤藤可以通过下调HIF-1α/VEGF信号通路影响HepG2肝癌细胞的侵袭转移并促进其凋亡，从而减少肿瘤区域的血管生成。
Abstract:
Objective:To investigate the effect of Yao medicine tumor vine on HepG2 hepatocellular carcinoma cells through HIF-1α/VEGF signaling pathway in order to explore the possible mechanism of its effect.Methods:The hypoxic microenvironment of liver cancer cells was simulated in vitro to prepare low,medium and high dose drug-containing serum of tumor rattan.They were randomly divided into normoxic model group,hypoxic model group,tumor rattan low,medium and high dose groups.CCK-8 method was used to detect the cell activity of each group.Hoechst33342 staining method was used to detect the apoptosis of each group.Transwell assay was used to detect the cell migration ability.Immunofluorescence chemical staining was used to detect the expression of HIF-1α and VEGF positive cells,flow cytometry was used to detect the apoptosis ratio,and RT-qPCR and Western blot were used to detect the expression levels of HIF-1α,VEGF,and Caspase-1 mRNA and proteins in each group of cells.Results:Compared with the normoxia model group,the cell activity of the hypoxia model group increased,the apoptosis ratio decreased,the number of migrating cells increased,and the expression levels of HIF-1α,VEGF and Caspase-1 mRNA and protein increased (all P<0.05);compared with the model group,the cell activity of the tumor vine-containing serum in each dosage group decreased,the apoptosis ratio increased,the number of migrating cells decreased,and the expression levels of HIF-1α,VEGF and Caspase-1 mRNA and protein expression decreased,and the middle dose group had the best effect (all P<0.05).Conclusion:The tumor rattan of Yao medicine can affect the invasion and metastasis of HepG2 liver cancer cells and promote their apoptosis by down-regulating the HIF-1α/VEGF signaling pathway,thereby reducing angiogenesis in the tumor area.

参考文献/References:

[1]陈静,徐蕾,曹正民,等.中医药抗肝纤维化细胞分子机制研究进展[J].中华中医药杂志,2022,37(3):1564-1569.
[2]钟长军,王秀.原发性肝癌中西医结合治疗及危险因素研究现状[J].锦州医科大学学报,2019,40(4):106-108.
[3]耿修来,刘莹,邹金婷,等.1990至2019年海南省肝癌疾病负担变化趋势及其危险因素分析[J].现代肿瘤医学,2024,32(19):3769-3775.
[4]陈莉莉,赵毅军,钱虹,等.2019年甘肃省肿瘤登记地区肝癌流行特征及2010—2019年变化趋势分析[J].中国慢性病预防与控制,2024,32(7):493-498.
[5]杨朗,陈晓,蔡建强,等.病因和肝病背景对肝细胞癌综合治疗策略的影响[J].中国实用外科杂志,2024,44(9):1005-1009.
[6]李泽洋,罗湄,郭松.瑶药肿瘤藤多酚提取工艺优化及其抗菌和抗氧化活性分析[J].化学研究与应用,2022,34(12):2927-2934.
[7]王云卿,马国需,赵丹,等.瑶药星毛冠盖藤正丁醇部位化学成分研究[J].中国药学杂志,2017,52(18):1580-1584.
[8]冯浩,杜倩倩,冯志强,等.特异性靶向肿瘤组织的IDO1抑制剂前药的研究[J].药学学报,2020,55(5):958-966.
[9]张庆磊,刘霞,孙卓宽,等.欧前胡素调节HIF-1α/VEGF信号通路对膀胱癌细胞上皮间质转化和血管生成的影响[J].中国老年学杂志,2024,44(20):5050-5054.
[10]史梦真,白玉婷,周白丽,等.高原地区不同程度冠状动脉狭窄与EGR3、VEGF蛋白的相关性分析[J].实用心电学杂志,2024,33(5):438-443，453.
[11]孟繁杰,马青嵩,陈曼丽,等.瑞香素调节缺氧诱导因子-1α/血管内皮生长因子信号通路对胫骨骨折大鼠骨痂形成和血管生成的影响实验研究[J].陕西医学杂志,2024,53(9):1182-1186，1192.
[12]王媛媛,顾玉,刘秋霞,等.落新妇苷通过调节HIF-1α/VEGF轴抑制乳腺癌细胞的增殖、迁移和血管生成拟态形成[J].中国肿瘤生物治疗杂志,2024,31(8):796-802.
[13]江巍,陈瑞,刘先姜.真武汤联合穴位敷贴治疗原发性肝癌合并腹水的效果[J].保健医学研究与实践,2023,20(5):57-60.
[14]杨丽,张荣臻,吴智鹏,等.柴芍六君子汤在原发性肝癌中的应用进展[J].中医研究,2024,37(8):93-96.
[15]班宜奎,任庆华,金娜英,等.中西医关于肝的认识差异及其根源[J].河南中医,2024,44(10):1542-1546.
[16]黄祎,刘华宝.“肝为气之治”理论论治肝癌的思路[J].中西医结合肝病杂志,2024,34(4):289-291.
[17]莫苑君,卢旭全,陆运鑫.中医药治疗中晚期肝癌研究进展[J].广西中医药,2024,47(5):74-78.
[18]王云卿,马国需,梁琼平,等.瑶药“肿瘤藤”(星毛冠盖藤)的化学成分研究[J].中草药,2016,47(24):4326-4330.
[19]丁月超,王谦,姚全军,等.PD-1抑制剂及抗血管生成药物联合TACE治疗原发性肝癌的效果[J].河南医学研究,2024,33(16):3023-3027.
[20]COWMAN S J,KOH M Y.Revisiting the HIF switch in the tumor and its immune microenvironment[J].Trends Cancer,2022,8(1):28-42.
[21]陈嵘,李淑敏,李旭华,等.软坚散结方调控小鼠HIF-1α糖酵解分子转导抑制肝肿瘤细胞增殖机制研究[J].陕西中医,2023,44(7):839-842，848.
[22]胡建文,冯群虎,王业皇,等.王氏生肌散对肛周脓肿大鼠创面愈合及HIF-1α/VEGF信号通路的影响[J].陕西中医,2023,44(9):1179-1183.
[23]PALAZON A,TYRAKIS P A,MACIAS D,et al.An HIF-1α/VEGF-A axis in cytotoxic T cells regulates tumor progression[J].Cancer Cell,2017,32(11):669-683.
[24]APTE R S,CHEN D S,FERRARA N.VEGF in signaling and disease: Beyond discovery and development[J].Cell，2019,176(3):1248-1264.
[25]齐淑静,付改霞,齐瑞霞.小檗碱调节HIF-1α/VEGF信号通路对皮肤溃疡大鼠血管生成和创面愈合的影响[J].陕西医学杂志,2024,53(2):173-178.

备注/Memo

备注/Memo:
国家自然科学基金资助项目（81860790）；广西中医药大学博士研究生创新项目（YCBXJ2023031）
更新日期/Last Update: 2025-06-09