[1]孙一丹,王慧敏,吴邈,等.基于PI3K/AKT信号通路探讨石芦清金颗粒对脂多糖致小鼠急性肺损伤的保护作用机制[J].陕西中医,2025,46(6):751-755,781.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.006]
　SUN Yidan,WANG Huimin,WU Miao,et al.Exploring the protective mechanism of Shilu Qingjin granules against lipopolysaccharide-induced acute lung injury in mice via the PI3K/AKT signaling pathway[J].,2025,46(6):751-755,781.[doi:DOI:10.3969/j.issn.1000-7369.2025.06.006]
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基于PI3K/AKT信号通路探讨石芦清金颗粒对脂多糖致小鼠急性肺损伤的保护作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年6期
页码:
751-755,781
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
Exploring the protective mechanism of Shilu Qingjin granules against lipopolysaccharide-induced acute lung injury in mice via the PI3K/AKT signaling pathway
作者:
孙一丹1; 王慧敏1; 吴邈1; 冯曾淇1; 黄博杰1; 杨曼曼1; 屈小元2
（1.陕西中医药大学，陕西 咸阳 712046；2.陕西省中医医院，陕西 西安 710003）
Author(s):
SUN Yidan1; WANG Huimin1; WU Miao1; FENG Zengqi1; HUANG Bojie1; YANG Manman1; QU Xiaoyuan2
（1.Shaanxi University of Chinese Medicine，Xianyang 712046，China；2.Shaanxi Provincial Hospital of Traditional Chinese Medicine，Xi’an 710003，China）
关键词:
急性肺损伤; 石芦清金颗粒; 磷脂酰肌醇-3-激酶/蛋白激酶B信号通路; 炎症因子; 炎症反应; 小鼠
Keywords:
Acute lung injury; Shilu Qingjin granules; PI3K/AKT signaling pathway; Inflammatory factors; Inflammatory response; Mice
分类号:
R 563
DOI:
DOI:10.3969/j.issn.1000-7369.2025.06.006
文献标志码:
A
摘要:
目的：探讨石芦清金颗粒通过调节磷脂酰肌醇-3-激酶/蛋白激酶B（PI3K/AKT）信号通路对脂多糖诱导的急性肺损伤（ALI）小鼠的预防和保护作用机制。方法：将雄性C57BL/6小鼠随机分为空白组、甲泼尼龙片阳性对照组、模型组、石芦清金颗粒低剂量组、石芦清金颗粒中剂量组、石芦清金颗粒高剂量组，每组各10只。各组分别给予同体积0.9%氯化钠溶液、相应浓度石芦清金颗粒和甲泼尼龙灌胃。给药7 d后，采用气管滴注脂多糖的方法建立ALI模型。观察各组小鼠的一般状态情况；HE染色法观察肺组织病理变化；ELISA法检测小鼠血清中肿瘤坏死因子（TNF-α）、白细胞介素-10（IL-10）、白细胞介素-1α（IL-1α）、转化生长因子-β（TGF-β）的水平；Western blot法检测小鼠肺组织PI3K、p-PI3K、AKT、p-AKT蛋白表达情况。结果：与空白组比较，模型组小鼠肺间质内可见大量炎性细胞浸润、肺泡结构完整性破坏等典型ALI病理改变，血清中TNF-α、IL-1α水平明显升高（P<0.01），IL-10和TGF-β水平明显降低（P<0.01），p-PI3K、p-AKT蛋白表达水平明显升高（P<0.01）。与模型组比较，甲泼尼龙片阳性对照组与石芦清金颗粒低、中、高剂量各组小鼠肺组织结构破坏现象明显被改善，炎性细胞浸润减少，TNF-α、IL-1α水平明显降低（P<0.01），IL-10水平明显升高（P<0.01），甲泼尼龙片阳性对照组与石芦清金颗粒中、高剂量各组小鼠血清中TGF-β含量均明显升高(P<0.01)，低剂量组小鼠血清中TGF-β含量升高(P<0.05)；随着对小鼠进行干预治疗所使用的石芦清金颗粒剂量的增高，其p-PI3K、p-AKT蛋白表达水平有显著的下降趋势(P<0.01)。结论：石芦清金颗粒对ALI小鼠有保护作用，其机制可能与调控PI3K/AKT信号通路有关。
Abstract:
Objective:To investigate the preventive and protective mechanisms of Shilu Qingjin granules against lipopolysaccharide-induced acute lung injury (ALI) in mice through regulation of the PI3K/AKT signaling pathway.Methods:Male C57BL/6 mice were randomly divided into six groups (n=10 per group):blank control group,methylprednisolone-treated positive control group,model group,and Shilu Qingjin granules low-,medium-,and high-dose groups.All groups received equal volumes of normal saline,corresponding concentrations of Shilu Qingjin granules,or methylprednisolone via oral gavage.After 7 days of administration,ALI models were established through intratracheal instillation of lipopolysaccharide.General physical status was observed in all groups.Pathological changes in lung tissue were examined using HE staining.Serum levels of TNF-α,IL-10,IL-1α,and TGF-β were measured by ELISA.Western blot analysis was performed to detect PI3K,p-PI3K,AKT,and p-AKT protein expression in lung tissue.Results:Compared with the control group,the model group exhibited typical pathological features of acute lung injury (ALI),including extensive inflammatory cell infiltration in pulmonary interstitium and disruption of alveolar structural integrity.Serum levels of TNF-α and IL-1α were significantly elevated (P<0.01),while IL-10 and TGF-β levels were markedly reduced (P<0.01).Additionally,protein expression levels of p-PI3K and p-AKT showed significant increases (P<0.01).In comparison to the model group,both the methylprednisolone-treated positive control group and Shilu Qingjin granules low-,medium-,and high-dose groups demonstrated substantial improvements in pulmonary tissue structure and reduced inflammatory cell infiltration.TNF-α and IL-1α levels were significantly decreased (P<0.01),accompanied by markedly elevated IL-10 levels (P<0.01).The methylprednisolone-treated positive control group and medium/high-dose Shilu Qingjin granules groups showed significant increases in serum TGF-β levels (P<0.01),while the low-dose group exhibited a moderate elevation (P<0.05).Notably,a dose-dependent inhibition of p-PI3K and p-AKT protein expression was observed in Shilu Qingjin granules-treated groups,with higher doses demonstrating more pronounced reductions (P<0.01).Conclusion:Shilu Qingjin granules demonstrate protective effects against ALI in mice,potentially mediated through regulation of the PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
陕西省科学技术厅重点研发计划项目（2020SF-344）；陕西省中医药管理局科研项目（SZY-KJCYC-2025-JC-044，LCPT019）
更新日期/Last Update: 2025-06-09