[1]左巧娟,谭劲,肖艳波,等.加味丹玄口康调节Hippo/YAP信号通路对大鼠口腔黏膜下纤维化的影响[J].陕西中医,2025,46(8):1011-1015.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.001]
　ZUO Qiaojuan,TAN Jin,XIAO Yanbo,et al.Effect of Jiawei Danxuan Koukang on oral submucosal fibrosis in rats by regulating Hippo/YAP signaling pathway[J].,2025,46(8):1011-1015.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.001]
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加味丹玄口康调节Hippo/YAP信号通路对大鼠口腔黏膜下纤维化的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年8期
页码:
1011-1015
栏目:
基础研究
出版日期:
2025-08-05

文章信息/Info

Title:
Effect of Jiawei Danxuan Koukang on oral submucosal fibrosis in rats by regulating Hippo/YAP signaling pathway
作者:
左巧娟; 谭劲; 肖艳波; 刘寻; 陈世娟; 李群
（湖南中医药大学第一附属医院，湖南 长沙 410007）
Author(s):
ZUO Qiaojuan; TAN Jin; XIAO Yanbo; LIU Xun; CHEN Shijuan; LI Qun
（First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007，China）
关键词:
口腔黏膜下纤维化; 加味丹玄口康; Hippo; Yes相关蛋白; 信号通路; 大鼠
Keywords:
Oral submucosal fibrosis; Jiawei Danxuan Koukang; Hippo; Yes-related protein; Signaling pathway; Rat
分类号:
R 781.5
DOI:
DOI:10.3969/j.issn.1000-7369.2025.08.001
文献标志码:
A
摘要:
目的：探讨加味丹玄口康（DXKK）调节Hippo/Yes相关蛋白（YAP）信号通路对大鼠口腔黏膜下纤维化（OSF）的影响。方法：构建OSF大鼠模型，将建模成功的大鼠分为OSF组、L-DXKK、M-DXKK、H-DXKK（灌胃4、8、12 ml/kg的DXKK）、H-DXKK+PY-60组（灌胃12 ml/kg的DXKK+尾静脉注射16 mg/kg的Hippo/YAP信号通路激活剂PY-60），每组各10只。另取10只正常大鼠为Control组，Control组与OSF组给予等量0.9%氯化钠溶液，1次/d，连续灌胃28 d。游标卡尺测量大鼠张口度；HE染色观察颊黏膜组织病理变化；Masson染色观察胶原纤维沉积情况；ELISA检测转化生长因子β1（TGF-β1）、Ⅲ型胶原蛋白（ColⅢ）和干扰素-γ（IFN-γ）水平；Western blot检测颊黏膜组织p-MST1/MST1、p-YAP/YAP蛋白水平。结果：OSF组大鼠较Control组颊黏膜上皮明显萎缩，上皮钉突变平，有大量胶原纤维沉积；L-DXKK组、M-DXKK组、H-DXKK组大鼠较OSF组黏膜上皮层增厚，胶原纤维沉积减少；H-DXKK+PY-60组大鼠较H-DXKK组黏膜上皮可见病理改变，上皮层胶原纤维沉积增多。OSF组大鼠张口度小于Control组，颊黏膜评分、TGF-β1、ColⅢ和IFN-γ水平、p-MST1/MST1、p-YAP/YAP蛋白表达高于Control组（均P＜0.05）；L-DXKK组、M-DXKK组、H-DXKK组大鼠张口度大于OSF组，颊黏膜评分、TGF-β1、ColⅢ和IFN-γ水平、p-MST1/MST1、p-YAP/YAP蛋白表达低于OSF组（均P＜0.05）；H-DXKK+PY-60组大鼠张口度小于H-DXKK组，颊黏膜评分、TGF-β1、ColⅢ和IFN-γ水平、p-MST1/MST1、p-YAP/YAP蛋白表达高于H-DXKK组（均P＜0.05）。结论：DXKK可通过抑制Hippo/YAP信号通路改善大鼠OSF。
Abstract:
Objective:To investigate the effect of Jiawei Danxuan Koukang (DXKK) on oral submucosal fibrosis (OSF) in rats by regulating Hippo/YAP signaling pathway.Methods:The OSF rat model was constructed,and successfully modeled rats were grouped into OSF group,L-DXKK,M-DXKK,H-DXKK (gavage of 4,8,and 12 ml/kg DXKK),and H-DXKK+PY-60 group (gavage of 12 ml/kg DXKK+tail vein injection of 16 mg/kg Hippo/YAP signaling pathway activator PY-60),with 10 rats in each group.Another 10 normal rats were designated as control group.The control group and the OSF group were given the same amount of 0.9% sodium chloride solution once a day for continuous gavage for 28 days.Measure the mouth opening degree of rats with a vernier caliper.The pathological changes of buccal mucosa tissue were observed by HE staining.Masson staining was used to observe the deposition of collagen fibers.ELISA was used to detect the levels of transforming growth factor-β1 (TGF-β1),type Ⅲ collagen (ColⅢ),and interferon-γ (IFN-γ).The protein levels of p-MST1/MST1 and p-YAP/YAP in buccal mucosal tissues were detected by Western blot.Results:The buccal mucosal epithelium of rats in the OSF group showed significant atrophy compared to the control group,with flattened epithelial ridges and a large amount of collagen fiber deposition.Rats in the L-DXKK,M-DXKK,and H-DXKK groups exhibited thickened mucosal epithelium and reduced collagen fiber deposition compared to the OSF group.In the H-DXKK+PY-60 group,pathological changes were observed in the mucosal epithelium compared to the H-DXKK group,with increased collagen fiber deposition.The mouth opening of rats in the OSF group was smaller than that of the control group,while the buccal mucosa scores,levels of TGF-β1,ColⅢ,and IFN-γ,as well as the expression of p-MST1/MST1 and p-YAP/YAP proteins were higher than those in the control group (all P<0.05).Rats in the L-DXKK,M-DXKK,and H-DXKK groups had a greater mouth opening than those in the OSF group,and their buccal mucosa scores,TGF-β1,ColⅢ,and IFN-γ levels,along with p-MST1/MST1 and p-YAP/YAP protein expression were lower than those in the OSF group (all P<0.05).Rats in the H-DXKK+PY-60 group had a smaller mouth opening than those in the H-DXKK group,and their buccal mucosa scores,TGF-β1,ColⅢ and IFN-γ levels,and p-MST1/MST1 and p-YAP/YAP protein expression were higher than those in the H-DXKK group (all P<0.05).Conclusion:DXKK may improve rat OSF by inhibiting the Hippo/YAP signaling pathway.

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[1]王宗康,肖艳波,刘 寻,等.加味丹玄口康调控口腔黏膜下纤维化大鼠Wnt/β-catenin信号通路机制研究[J].陕西中医,2022,(6):687.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.002]
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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82374530）；湖南中医药大学校院联合基金资助项目（2024XYLH008）
更新日期/Last Update: 2025-08-08