[1]张普,侯婷婷,武亦阁,等.调营达卫布津方对衰老人角质形成细胞线粒体功能及AMPK/PGC-1α信号通路的影响[J].陕西中医,2025,46(8):1016-1022.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.002]
　ZHANG Pu,HOU Tingting,WU Yige,et al.Effects of Tiaoying Dawei Bujin recipe on mitochondrial function and AMPK/PGC-1α signaling pathway in aging human keratinocytes[J].,2025,46(8):1016-1022.[doi:DOI:10.3969/j.issn.1000-7369.2025.08.002]
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调营达卫布津方对衰老人角质形成细胞线粒体功能及AMPK/PGC-1α信号通路的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年8期
页码:
1016-1022
栏目:
基础研究
出版日期:
2025-08-05

文章信息/Info

Title:
Effects of Tiaoying Dawei Bujin recipe on mitochondrial function and AMPK/PGC-1α signaling pathway in aging human keratinocytes
作者:
张普1; 侯婷婷2; 武亦阁3; 李彤1; 刘爱玲1; 周光1
（1.新疆医科大学中医学院，新疆 乌鲁木齐 830054；2.新疆医科大学第五附属医院，新疆 乌鲁木齐 830054；3.深圳市中西医结合医院，广东 深圳 518100）
Author(s):
ZHANG Pu1; HOU Tingting2; WU Yige3; LI Tong1; LIU Ailing1; ZHOU Guang1
(1.Xinjiang Medical University Institute of TCM,Urumqi 830054，China;2.The Fifth Affiliated Hospital of Xinjiang Medical University,Urumqi 830054，China;3.Shenzhen Hospital of Integrative Medicine,Shenzhen 518100，China)
关键词:
调营达卫布津方; 补益肝肾; 皮肤衰老; 角质形成细胞; 线粒体功能; AMPK/PGC-1α信号通路
Keywords:
Tiaoying Dawei Bujin recipe; Reinforcing liver benefiting kidney; Skin aging; Keratinocyte; Mitochondrial function; AMPK/PGC-1α signaling pathway
分类号:
R 285
DOI:
DOI:10.3969/j.issn.1000-7369.2025.08.002
文献标志码:
A
摘要:
目的：探究调营达卫布津方对衰老人角质形成细胞线粒体功能及AMPK/PGC-1α信号通路的影响。方法：将细胞分为空白组、模型组、调营达卫布津方低剂量、中剂量和高剂量组，空白组加入完全培养基干预48 h，模型组加入2.0 mmol/L AAPH干预48 h，调营达卫布津方组在加入不同浓度药物干预48 h前2 h加入2.0 mmol/L AAPH，调营达卫布津方低、中、高剂量组药物干预浓度分别为10、20、40 μg/ml。通过ELISA、免疫荧光法及透射电镜探究调营达卫布津方对衰老HaCat细胞氧化应激、线粒体膜电位及形态的影响；利用Western blot检测调营达卫布津方对衰老细胞AMPK/PGC-1α信号通路蛋白表达及衰老相关蛋白P53表达的影响。结果：模型组ROS、MDA、IL-6含量相较于空白组显著升高（均P＜0.01），SOD的含量显著下降（P＜0.01），P53蛋白表达量显著升高（P＜0.01），AMPK、PGC-1α蛋白表达量明显下降（均P＜0.05）；模型组异常线粒体膜电位表达水平高于空白组，线粒体膜破损，基质外溢，嵴断裂、紊乱，自噬水平居中。调营达卫布津方高剂量组ROS、MDA、IL-6含量相较于模型组显著下降，SOD含量显著升高（均P＜0.01），P53表达明显下降（P＜0.05），AMPK、PGC-1α蛋白表达明显升高（均P＜0.05）；调营达卫布津方组线粒体膜电位表达水平增强，线粒体形态完整，嵴断裂减少，自噬溶酶体增多。结论：调营达卫布津方可通过提高衰老HaCat细胞氧化应激水平，减轻炎症反应，改善线粒体形态及功能达到改善皮肤衰老的目的。
Abstract:
Objective:To explore the effects of Tiaoying Dawei Bujin recipe on mitochondrial function and AMPK/PGC-1α signaling pathway in aging human keratinocytes.Methods:The cells were divided into blank group,model group,low dose group,medium dose group and high dose group.The blank group was added with complete medium for 48 hours.The model group was added with 2.0 mmol/L AAPH for 48 hours.The Tiaoying Dawei Bujin recipe groups were added with 2.0 mmol/L AAPH 2 hours before adding different concentrations of drugs for 48 hours.The concentrations of low,medium and high dose groups were 10,20 and 40 μg/ml respectively.ELISA,immunofluorescence and transmission electron microscopy were used to explore the effects of Tiaoying Dawei Bujin recipe on oxidative stress,mitochondrial membrane potential and morphology of senescent HaCat cells.Wb was used to detect the effect of Tiaoying Dawei Bujin recipe on the expression of AMPK/PGC-1α signaling pathway protein and the expression of aging-related protein P53 in senescent cells.Results:The contents of ROS,MDA and IL-6 in the model group were significantly higher than those in the blank group (all P<0.01),the content of SOD was significantly decreased (P<0.01),the expression of P53 protein was significantly increased (P<0.01),and the expression of AMPK and PGC-1α protein was significantly decreased (all P<0.05).The expression level of abnormal mitochondrial membrane potential in the model group was higher than that in the blank group.The mitochondrial membrane was damaged,the matrix overflowed,the ridge was broken and disordered,and the autophagy level was in the middle.Compared with the model group,the contents of ROS,MDA and IL-6 in the high-dose group of Tiaoying Dawei Bujin decoction were significantly decreased,the content of SOD was significantly increased (all P<0.01),the expression of P53 protein was significantly decreased (P<0.05),the expression of AMPK and PGC-1α protein was significantly increased (all P<0.05),the expression level of mitochondrial membrane potential was enhanced,the mitochondrial morphology was complete,the ridge fracture was reduced,and the autophagic lysosome was increased.Conclusion:Tiaoying Dawei Bujin recipe can improve the oxidative stress level of aging HaCat cells,reduce the inflammatory response,and improve the morphology and function of mitochondria to improve skin aging.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81960825）
更新日期/Last Update: 2025-08-08