[1]罗玉芳,杨军平,李怀玉,等.肾衰泄浊汤对慢性肾衰大鼠肾脏巨噬细胞向肌成纤维细胞转换的影响[J].陕西中医,2025,46(12):1604-1609.[doi:DOI:10.3969/j.issn.1000-7369.2025.12.004]
　LUO Yufang,YANG Junping,LI Huaiyu,et al.Effect of Shenshuai Xiezhuo decoction on the transition of renal macrophages to myofibroblasts in rats with chronic renal failure[J].,2025,46(12):1604-1609.[doi:DOI:10.3969/j.issn.1000-7369.2025.12.004]
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肾衰泄浊汤对慢性肾衰大鼠肾脏巨噬细胞向肌成纤维细胞转换的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
46
期数:
2025年12期
页码:
1604-1609
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Effect of Shenshuai Xiezhuo decoction on the transition of renal macrophages to myofibroblasts in rats with chronic renal failure
作者:
罗玉芳1; 杨军平1; 李怀玉2; 3; 吕森浩3; 李玮婷1
（1.江西中医药大学附属医院，江西 南昌 330006；2.广州中医药大学第一附属医院，广东 广州 510405；3.江西中医药大学，江西 南昌 330004）
Author(s):
LUO Yufang1; YANG Junping1; LI Huaiyu2; 3; LYU Senhao3; LI Weiting1
(1.Affiliated Hospital of Jiangxi University of Chinese Medicine,Nanchang 330006,China;2.The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China;3.Jiangxi University of Chinese Medicine,Nanchang 330004,China)
关键词:
肾纤维化; 肾衰泄浊汤; 巨噬细胞向肌成纤维细胞转换; miR-142-5p; JAK3/STAT6信号通路; 炎纤转化
Keywords:
Renal fibrosis; Shenshuai Xiezhuo decoction; Macrophage-to-myofibroblast transition; miR-142-5p; JAK3/STAT6 signaling pathway; Inflammation-fibrosis transformation
分类号:
R 692.5
DOI:
DOI:10.3969/j.issn.1000-7369.2025.12.004
文献标志码:
A
摘要:
目的：评估肾衰泄浊汤对腺嘌呤灌胃致慢性肾衰竭（CRF）大鼠模型肾脏中巨噬细胞向肌成纤维细胞转换（MMT）的影响，并从miR-142-5p/SOCS1/JAK3轴探究其可能作用机制。方法：将24只SD大鼠随机分为正常组、模型组、中药低浓度组和中药高浓度组，每组6只。除正常组大鼠外，其余组均采用腺嘌呤灌胃21天构建CRF模型。造模结束后，正常组和模型组给予0.9%氯化钠溶液，中药低浓度组和中药高浓度组给予相应浓度的肾衰泄浊汤，连续28 d。取材后，检测CRF大鼠血清中的血肌酐（Scr）、尿素氮（BUN）水平、白细胞介素-4（IL-4）和白细胞介素-13（IL-13）水平；HE染色和Masson染色观察肾组织的病理改变；免疫组化法检测肾组织的CD86、CD206、α-肌动蛋白（α-SMA）和Ⅰ型胶原蛋白（Collagen1）表达；qRT-PCR法检测肾组织的miR-142-5p表达，Western Blot法检测肾组织的SOCS1、JAK3、STAT6蛋白表达。结果：与模型组相比，肾衰泄浊汤能降低CRF大鼠的血清Scr、BUN、IL-4和IL-13水平，减轻肾脏的组织损害及纤维化改变，抑制肾组织中的CD86、CD206、α-SMA和Collagen1蛋白表达，下调miR-142-5p的表达，上调SOCS1并下调JAK3和STAT6的蛋白表达。结论：肾衰泄浊汤延缓肾纤维化的机制与MMT过程抑制有关，并与miRNA-142-5p/SOCS1/JAK3轴的调控有关。
Abstract:
Objective:To evaluate the effect of Shenshuai Xiezhuo decoction on macrophage-to-myofibroblast transition (MMT) in the kidneys of rats with chronic renal failure (CRF) induced by oral administration of adenine,and to explore the potential underlying mechanism via the miR-142-5p/SOCS1/JAK3 axis.Methods:Twenty-four Sprague-Dawley rats were randomly divided into four groups (n=6 per group):normal control group,model group,low-dose traditional Chinese medicine (TCM) group,and high-dose TCM group.Except for the normal group,CRF was induced in all other groups by oral administration of adenine for 21 days.After modeling,rats in the normal and model groups were administered normal saline,while those in the low-dose and high-dose TCM groups received Shenshuai Xiezhuo decoction at the corresponding concentrations for 28 consecutive days.After treatment,serum levels of serum creatinine (Scr),blood urea nitrogen (BUN),interleukin-4 (IL-4),and interleukin-13 (IL-13) were measured.Histopathological changes in the kidney tissues were observed using hematoxylin and eosin (HE) staining and Masson’s trichrome staining.Immunohistochemistry was performed to assess the expression of CD86,CD206,α-smooth muscle actin (α-SMA),and type Ⅰ collagen (Collagen Ⅰ) in renal tissues.The expression of miR-142-5p in kidney tissue was determined by qRT-PCR,and the protein expression levels of SOCS1,JAK3,and STAT6 were analyzed using Western Blotting.Results:Compared with the model group,Shenshuai Xiezhuo decoction significantly reduced serum levels of Scr,BUN,IL-4,and IL-13 in CRF rats,alleviated renal tissue injury and fibrosis,suppressed the renal expression of CD86,CD206,α-SMA,and Collagen I,downregulated the expression of miR-142-5p,upregulated SOCS1 protein levels,and downregulated the expression of JAK3 and STAT6.Conclusion:Shenshuai Xiezhuo decoction may delay renal fibrosis by inhibiting the MMT process,and its mechanism may be associated with regulation of the miR-142-5p/SOCS1/JAK3 signaling axis.

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备注/Memo

备注/Memo:
江西省自然科学基金资助重点项目（20232ACB206055）；江西省教育厅科学技术研究项目（GJJ211254）；江西省中医药管理局科技计划项目（2024B0267）
更新日期/Last Update: 2025-12-08