[1]乔佳俊,袁有才,马春林,等.新葛根芩连汤调控NF-κB通路改善AD小鼠神经炎症的机制研究[J].陕西中医,2026,(1):15-20.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.003]
　QIAO Jiajun,YUAN Youcai,MA Chunlin,et al.Mechanism study on the modulation of NF-κB pathway by Xingegen Qinlian decoction to ameliorate neuroinflammation in AD mice[J].,2026,(1):15-20.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.003]
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新葛根芩连汤调控NF-κB通路改善AD小鼠神经炎症的机制研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年1期
页码:
15-20
栏目:
基础研究
出版日期:
2026-01-05

文章信息/Info

Title:
Mechanism study on the modulation of NF-κB pathway by Xingegen Qinlian decoction to ameliorate neuroinflammation in AD mice
作者:
乔佳俊1; 袁有才1; 马春林1; 刘洋田子1; 唐宝丽2
（1.陕西中医药大学，陕西 咸阳 712046；2.空军军医大学第二附属医院神经内科，陕西 西安 710038）
Author(s):
QIAO Jiajun1; YUAN Youcai1; MA Chunlin1; LIU Yangtianzi1; TANG Baoli2
(1.Shaanxi University of Chinese Medicine,Xianyang 712046,China;2.Neurology Department,Tangdu Hospital,Air Force Military Medical University,Xi’an 710038,China)
关键词:
阿尔茨海默病; 新葛根芩连汤; 核因子-κB; 神经炎症; 白介素-6; 白介素-1β
Keywords:
Alzheimer’s disease; Xingegen Qinlian decoction; Nuclearfactor-kappa B; Neuroinflammation; Interleukin-6; Interleukin-1β
分类号:
R 74
DOI:
DOI:10.3969/j.issn.1000-7369.2026.01.003
文献标志码:
A
摘要:
目的：探讨新葛根芩连汤对APP/PS1小鼠核因子-κB（NF-κB）通路蛋白以及相关神经炎症分子的影响。方法：将APP swe/PS△E9小鼠随机分为模型组、新葛根芩连汤组、盐酸多奈哌齐组，同龄同族C57BL/6小鼠设置为空白对照组。分组干预后，记录其行为学改变。干预90 d后，水迷宫观察小鼠学习记忆能力、尼氏染色观察海马区神经元细胞形态、铅铀电镜观察海马区细胞超微结构、Western bolt法检测NF-κB蛋白表达、ELISA测定血清磷酸化tau蛋白（p-tau）蛋白含量、白介素-6（IL-6）、白介素-1β（IL-1β）的含量。结果：模型组与空白对照组相比小鼠逃避潜伏期明显增加(P<0.01)；小鼠海马神经元细胞结构模糊，有大量的空泡，胶质细胞增生；神经元胞体内细胞核核膜褶皱明显，线粒体呈现大量的空泡样，可见电子致密的纤维缠结，突触前膜与后膜模糊，突触小泡显著减少。NF-κB蛋白表达量增加，血清中的p-tau、IL-6、IL-1β含量显著上升（P＜0.05，P＜0.01）。与模型组相比，各药物干预组小鼠逃避潜伏期均缩短，训练至第3天和第5天时，差异具有统计学意义(P＜0.05)；海马神经元细胞结构较清晰，排列较整齐，胶质细胞少量增生；胞体内线粒体基本完整，细胞器分布均匀，胞体内偶见散在纤维，纤维缠结区域缩小，突触结构较清晰，突触小泡较为密集。海马区NF-κB蛋白表达水平下降；血清p-tau水平下降，其中新葛根芩连汤组下降更为显著（P＜0.01）；新葛根芩连汤组IL-1β水平上升幅度明显，且优于盐酸多奈哌齐组（P＜0.01）。结论：新葛根芩连汤改善AD小鼠认知功能的作用与其神经炎症减轻及tau蛋白磷酸化减少有关，NF-κB活性的抑制可能参与该过程。
Abstract:
Objective:To explore the effects of Xingegen Qinlian decoction on NF-κB pathway and related neuroinflammatory molecules in APP/PS1 mice.Methods:APP/PS1 mice were randomly divided into model group,Xingegen Qinlian decoction group,and Donepezil Hydrochloride group.Age- and strain-matched C57BL/6 mice were used as the control group.Behavioral changes were recorded after grouping interventions.After 90 days of intervention,the following assessments were conducted:the Morris water maze test to evaluate learning and memory abilities,Nissl staining to observe neuronal morphology in the hippocampus,Lead-uranium electron microscopy to observe ultrastructure of cells in the hippocampal region,Western blot to detect nuclear factor kappa-B (NF-κB) protein expression,and ELISA to measure serum levels of phosphorylated tau protein (p-tau),interleukin-6 (IL-6),and interleukin-1β(IL-1β).Results:Compared with the control group,the model group showed significantly prolonged escape latency (P<0.05);ill-defined hippocampal neuronal structures with extensive vacuoles and gliosis;nucleus nuclear membrane folds were obvious in neuronal intracellular bodies,mitochondria showed a large number of vacuole-like,electron-dense fiber tangles were visible,presynaptic and post-synaptic membranes were blurred,and synaptic vesicles were significantly reduced.Increased NF-κB protein expression,and elevated serum levels of p-tau,IL-6,and IL-1β (P<0.05).Compared with the model group,all drug intervention groups exhibited shorter escape latency,with statistically significant differences on days 3 and 5 of training (P<0.05).Most hippocampal neurons displayed clearer structures and orderly arrangements,with reduced gliosis.The mitochondria in the cytosol were basically intact,the organelles were uniformly distributed,scattered fibers were occasionally seen in the cytosol,the area of fiber entanglement was narrowed,the synaptic structure was clearer,and the synaptic vesicles were more densely packed.NF-κB protein expression in the hippocampus decreased,and serum p-tau levels declined,particularly in the Xingegen Qinlian decoction group (P<0.01).The Xingegen Qinlian decoction group also showed a more pronounced reduction in IL-1β levels compared to the Donepezil Hydrochloride group (P<0.01).Conclusion:The improvement of cognitive function by Xingegen Qinlian decoction group was associated with reduced neuroinflammation and decreased tau protein phosphorylation,and the inhibition of NF-κB activity may be involved in this process.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82374548）；陕西省自然科学基础研究计划（2021JQ-741）；陕西中医药大学研究生创新实践能力提升项目（CXSJ202405）；陕西省咸阳市科技局中医药创新项目（2024-36-87）
更新日期/Last Update: 2026-01-08