[1]钟磊,范娜,马龙,等.寒喘祖帕对过敏性哮喘小鼠的抗炎效果及其对肠道菌群结构调节作用研究[J].陕西中医,2026,(1):21-27.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.004]
　ZHONG Lei,FAN Na,MA Long,et al.Study on the anti-inflammatory effects and intestinal microbiota structure regulation of Hanchuan zupa in mice with allergic asthma[J].,2026,(1):21-27.[doi:DOI:10.3969/j.issn.1000-7369.2026.01.004]
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寒喘祖帕对过敏性哮喘小鼠的抗炎效果及其对肠道菌群结构调节作用研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年1期
页码:
21-27
栏目:
基础研究
出版日期:
2026-01-05

文章信息/Info

Title:
Study on the anti-inflammatory effects and intestinal microbiota structure regulation of Hanchuan zupa in mice with allergic asthma
作者:
钟磊1; 范娜2; 马龙1; 李鸿飞1; 宋春莉3
（1.北京儿童医院新疆医院 新疆维吾尔自治区儿童医院药学部，新疆 乌鲁木齐 830000；2.新疆医科大学第六附属医院输血科，新疆 乌鲁木齐 830002；3.新疆医科大学附属肿瘤医院药学部，新疆 乌鲁木齐 830011）
Author(s):
ZHONG Lei1; FAN Na2; MA Long1; LI Hongfei1; SONG Chunli3
(1.Department of Pharmacy,Children’s Hospital of Xinjiang Uygur Autonomous Region，Xinjiang Hospital of Beijing Children’s Hospital,Urumqi 830000,China;2.Department of Blood Transfusion,the Sixth Affiliated Hospital of Xinjiang Medical University,Urumqi 830002,China;3.Department of Pharmaceutical,Xinjiang Medical University Cancer Hospital,Urumqi 830011,China）
关键词:
过敏性哮喘; 寒喘祖帕; 抗炎药效学; 肠道菌群; “肠-肺”轴
Keywords:
Allergic asthma; Hanchuan Zupa; Anti-inflammatory pharmacodynamics; Gut microbiota; “Gut-lung” axis
分类号:
R 562.25
DOI:
DOI:10.3969/j.issn.1000-7369.2026.01.004
文献标志码:
A
摘要:
目的：本研究旨在探究寒喘祖帕对过敏性哮喘（AAS）小鼠模型的抗炎药效学作用以及对肠道菌群结构的调节效应。方法：采用卵蛋白（OVA）致敏和激发的方法建立AAS小鼠模型，分别给予地塞米松、寒喘祖帕进行灌胃给药干预，灌胃给药3周后使用动物肺功能仪检测各组小鼠的气道高反应性（AHR）。收集小鼠血液标本及支气管肺泡灌洗液（BALF），进行细胞计数分析，采用ELISA法检测BALF及血清中免疫球蛋白E（IgE）、肿瘤坏死因子-α（TNF-α）、白介素(IL)-4、IL-5、IL-6、IL-13的含量。取肺组织采用苏木精-伊红（HE）染色，观察小鼠肺组织病理变化。采用16S rRNA 测序技术分析小鼠肠道菌群的变化。结果：寒喘祖帕显著改善哮喘小鼠精神状态、活动度及体重，并延缓Penh值升高，降低AHR。病理学分析表明，寒喘祖帕可减轻气管上皮损伤、肺间隔增厚及炎症浸润。肠道菌群分析显示，寒喘祖帕显著提高Shannon和Simpson指数（P<0.05），恢复菌群α多样性。在门水平上，寒喘祖帕增加厚壁菌门、变形菌门等有益菌丰度，降低拟杆菌门比例；属水平上，普雷沃菌Prevotellaceae_UCG_001、阿克曼菌属Akkermansia等9个菌群被鉴定为关键生物标志物。结论：寒喘祖帕通过调节肠道菌群结构及多样性，缓解哮喘症状，其作用机制可能与菌群-免疫轴调控相关，为寒喘祖帕治疗哮喘提供了微生物组学依据。
Abstract:
Objective:This study aims to investigate the anti-inflammatory pharmacodynamic effects of Hanchuan Zupa on an allergic asthma (AAS) mouse model and its regulatory effects on the gut microbiota structure.Methods:An AAS mouse model was established using ovalbumin (OVA) sensitization and challenge.Mice were administered dexamethasone and hantazol via oral gavage.After three weeks of oral gavage,airway hyperresponsiveness (AHR) was assessed using an EMKA volume recorder.Blood samples and bronchoalveolar lavage fluid (BALF) were collected from the mice,and cell counts were analyzed.The levels of IgE,TNF-α,IL-4,IL-5,IL-6,and IL-13 in BALF and serum were detected using the ELISA method.Lung tissue was stained with hematoxylin and eosin (HE) to observe pathological changes in mouse lung tissue.The 16S rRNA sequencing technique was used to analyze changes in the intestinal microbiota of mice.Results:Hanchuan Zupa significantly improved the mental state,activity level,and body weight of asthmatic mice (P<0.05),delayed the increase in Penh values,and reduced airway hyperresponsiveness (AHR).Pathological analysis indicated that Hanchuan Zupa alleviated tracheal epithelial damage,pulmonary interstitial thickening,and inflammatory infiltration.Gut microbiota analysis showed that Hanchuan Zupa significantly increased the Shannon and Simpson indices and restored α diversity of the microbiota.At the phylum level,Hanchuan Zupa increased the abundance of beneficial bacteria such as Firmicutes and Proteobacteria,while reducing the proportion of Bacteroidetes;at the genus level,nine bacterial communities,including Prevotellaceae_UCG_001 and Akkermansia,were identified as key biomarkers.Conclusion:Hanchuan Zupa alleviates asthma symptoms by regulating the structure and diversity of the gut microbiota.Its mechanism of action may be related to the regulation of the microbiota-immune axis,providing microbiological evidence for the use of Hanchuan Zupa in the treatment of asthm.

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备注/Memo

备注/Memo:
新疆维吾尔自治区自然科学基金青年科学基金资助项目（2025D01B124,2022D01C804）
更新日期/Last Update: 2026-01-08