[1]温雅楠,赫吉铭,霍新慧,等.隔药饼灸对佐剂性关节炎大鼠滑膜组织相关蛋白表达的影响[J].陕西中医,2026,(2):147-153.[doi:DOI:10.3969/j.issn.1000-7369.2026.02.001]
　WEN Yanan,HE Jiming,HUO Xinhui,et al.Effect of moxibustion with medicinal cake on expression of related proteins in synovial tissue of adjuvant arthritis rats[J].,2026,(2):147-153.[doi:DOI:10.3969/j.issn.1000-7369.2026.02.001]
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隔药饼灸对佐剂性关节炎大鼠滑膜组织相关蛋白表达的影响

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年2期
页码:
147-153
栏目:
基础研究
出版日期:
2026-02-05

文章信息/Info

Title:
Effect of moxibustion with medicinal cake on expression of related proteins in synovial tissue of adjuvant arthritis rats
作者:
温雅楠1; 赫吉铭2; 霍新慧1; 兰永利1; 李雅萱1; 王晴1
（1.新疆医科大学中医学院，新疆 乌鲁木齐 830017；2.新疆医科大学第四临床医学院，新疆 乌鲁木齐 830000）
Author(s):
WEN Yanan1; HE Jiming2; HUO Xinhui1; LAN Yongli1; LI Yaxuan1; WANG Qing1
(1.College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830017,China;2.Fourth Clinical Medical College of Xinjiang Medical University,Urumqi 830000,China)
关键词:
类风湿关节炎; 刺山柑隔药饼灸; 佐剂性关节炎; 炎症因子; 核因子E2相关因子2; 溶质载体家族7成员11; 谷胱甘肽过氧化物酶4
Keywords:
Rheumatoid arthritis; Capparis spinosa cake-separated moxibustion; Adjuvant arthritis; Inflammatory factors; Nuclear factor erythroid 2-related factor 2; Solute carrier family 7 member 11; Glutathione peroxidase 4
分类号:
R 245
DOI:
DOI:10.3969/j.issn.1000-7369.2026.02.001
文献标志码:
A
摘要:
目的：观察新疆特色药材刺山柑隔药饼灸对佐剂性关节炎（AA）大鼠左后肢足跖炎症程度评分、足爪容积，血清肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）水平，以及滑膜组织核因子 E2 相关因子 2（Nrf2）、溶质载体家族 7 成员 11（SLC7A11）、谷胱甘肽过氧化物酶 4（GPX4）蛋白表达的影响，探究其对类风湿关节炎（RA）的作用机制。方法：32 只 SD 大鼠随机取 8 只为正常组，其余 24 只于左足跖注射完全弗氏佐剂（0.1 ml/只）建立AA模型。成模后将 24 只AA大鼠随机分为模型组、隔假药饼灸组、刺山柑隔药饼灸组，每组 8 只。干预组于双侧足三里穴施灸，每日 1 次，每穴 5 壮，连续10 d；模型组仅抓取固定。分别于造模后3 d、干预5、10 d后测量足跖炎症程度评分及足爪容积。干预结束后次日取材，采用酶联免疫吸附法（ELISA）检测血清炎症因子水平；苏木精-伊红染色观察组织病理变化；免疫组化法和蛋白质印迹法检测滑膜组织蛋白表达。结果：干预前，模型组较正常组足跖评分、足爪容积及血清炎症因子水平升高（均P＜0.05）；病理损伤严重，滑膜上述蛋白表达降低（均P＜0.05）。干预后，模型组上述指标仍异常（均P＜0.05）；刺山柑隔药饼灸组较模型组足跖评分、足爪容积及血清炎症因子水平降低（均P＜0.05）；病理损伤改善，滑膜蛋白表达升高（均P＜0.05）。结论：刺山柑隔药饼灸可降低AA大鼠血清炎症因子水平，上调滑膜 Nrf2、SLC7A11 及GPX4蛋白表达，减轻关节炎症及软骨病理损伤，通过调节氧化应激途径治疗RA，为RA提供了中医外治方案。
Abstract:
Objective:The effects of Xinjiang characteristic medicinal herb,Zanthoxylum bungeanum,interposed medicinal cake moxibustion on the degree of inflammation in the left hind paw plantar of adjuvant arthritis (AA) rats,paw volume,serum levels of tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),and interleukin-6 (IL-6),as well as the protein expression of nuclear factor erythroid 2-related factor 2 (Nrf2),solute carrier family 7 member 11 (SLC7A11),and glutathione peroxidase 4 (GPX4) in the synovial tissue were observed to explore its mechanism of action on rheumatoid arthritis (RA).Methods:32 SD rats were randomly divided into a normal group (8 rats) and an AA model group (24 rats).The AA model was established by injecting complete Freund’s adjuvant (0.1 ml/rat) into the left hind paw plantar of the rats.After modeling,the 24 AA rats were randomly divided into model group,sham moxibustion group,and Zanthoxylum bungeanum interposed medicinal cake moxibustion group,with 8 rats in each group.The intervention groups received moxibustion at bilateral Zusanli acupoints once a day,5 moxa cones per point,for 10 consecutive days;the model group was only grasped and fixed.The degree of inflammation in the paw plantar and paw volume were measured at 3 days after modeling and 5 and 10 days after intervention.The rats were sacrificed the day after the intervention,and the serum levels of inflammatory factors were detected by enzyme-linked immunosorbent assay.Hematoxylin-eosin staining was used to observe the histopathological changes.The protein expression in the synovial tissue was detected by immunohistochemistry and Western blot.Results:Before intervention,the paw plantar score,paw volume,and serum inflammatory factor levels in the model group were higher than those in the normal group (all P＜0.05).The pathological damage was severe,and the expression of the above proteins in the synovial tissue was decreased (all P＜0.05).After intervention,the above indicators in the model group remained abnormal (all P＜0.05).The paw plantar score,paw volume,and serum inflammatory factor levels in the Zanthoxylum bungeanum interposed medicinal cake moxibustion group were lower than those in the model group (all P＜0.05).The pathological damage was improved,and the protein expression in the synovial tissue was increased (all P＜0.05).Conclusion:Zanthoxylum bungeanum interposed medicinal cake moxibustion can reduce the serum inflammatory factor levels in AA rats,up-regulate the expression of Nrf2,SLC7A11,and GPX4 proteins in the synovial tissue,alleviate joint inflammation and cartilage pathological damage,and treat RA by regulating the oxidative stress pathway,providing a traditional Chinese medicine external treatment option for RA.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81860890）
更新日期/Last Update: 2026-02-09