[1]王紫寒,王子强,帖昊奇,等.痔疮安膏通过抑制NLRP3炎症小体通路发挥抗炎镇痛作用机制研究[J].陕西中医,2026,(4):449-454.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.003]
　WANG Zihan,WANG Ziqiang,TIE Haoqi,et al.Study on the anti-inflammatory and analgesic effects and mechanisms of Zhichuang’an ointment through inhibiting the NLRP3 inflammasome pathway[J].,2026,(4):449-454.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.003]
点击复制

痔疮安膏通过抑制NLRP3炎症小体通路发挥抗炎镇痛作用机制研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年4期
页码:
449-454
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
Study on the anti-inflammatory and analgesic effects and mechanisms of Zhichuang’an ointment through inhibiting the NLRP3 inflammasome pathway
作者:
王紫寒1; 2; 王子强1; 2; 帖昊奇1; 2; 张雪1; 2; 史永恒1; 2; 王川1; 2; 刘继平1; 2; 张军武1; 3; 卫昊1; 2
（1.陕西中医药大学，陕西 咸阳 712046；2.陕西省中医药管理局中药药效机制与物质基础重点研究室，陕西 咸阳 712046；3.陕西陕中药业有限公司，陕西 咸阳 712083）
Author(s):
WANG Zihan1; 2; WANG Ziqiang1; 2; TIE Haoqi1; 2; ZHANG Xue1; 2; SHI Yongheng1; 2; WANG Chuan1; 2; LIU Jiping1; 2; ZHANG Junwu1; 3; WEI Hao1; 2
（1.Shaanxi University of Chinese Medicine，Xianyang 712046，China；2.Key Laboratory of Pharmacodynamics and Material Basis of Chinese Medicine of Shaanxi Administration of Traditional Chinese Medicine，Xianyang 712046，China；3.Shaanxi Shanzhong Pharmaceutical Co.Ltd.，Xianyang 712083，China）
关键词:
痔疮安膏; NLRP3炎症小体; 抗炎; 镇痛; 肛周肿胀; 醋酸致扭体反应
Keywords:
Zhichuang’an ointment; NLRP3 inflammasome; Anti-inflammatory; Analgesi; Perianal swelling; Acetic acid-induced writhing
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2026.04.003
文献标志码:
A
摘要:
目的：探讨痔疮安膏的抗炎镇痛作用及其基于核苷酸结合寡聚化结构域样受体蛋白3（NLRP3）炎症小体通路的作用机制。方法：采用巴豆油致大鼠肛周肿胀模型评价局部抗炎作用；通过二甲苯致小鼠耳肿胀、角叉菜胶致大鼠足肿胀及醋酸致小鼠扭体模型，分别评价全身抗炎与镇痛作用。采用冰醋酸诱导大鼠痔疮模型，HE染色与Masson染色观察肛周组织病理与纤维化；ELISA法检测血清炎症因子肿瘤坏死因子-α（TNF-α）、白介素（IL）-6、IL-1β、IL-18水平；Western blot检测肛周组织NLRP3、凋亡相关斑点样蛋白（ASC）、半胱氨酸天冬氨酸蛋白酶1（Caspase-1）及IL-1β蛋白表达。结果：痔疮安膏能剂量依赖性地抑制各类炎性肿胀及扭体反应（P<0.01或P<0.05）。痔疮安膏可显著减轻痔疮模型大鼠肛周组织炎细胞浸润、水肿及胶原沉积，降低血清促炎因子水平，并抑制NLRP3通路关键蛋白的表达（P<0.01或P<0.05）。结论：痔疮安膏具有明确的抗炎镇痛作用，其机制可能与抑制NLRP3炎症小体通路活化、减少促炎因子释放及改善组织纤维化有关。
Abstract:
Objective:To examine the anti-inflammatory and analgesic properties of Zhichuang’an ointment and its potential mechanism involving the NLRP3 inflammasome pathway.Methods:Anti-inflammatory activity was assessed using established models:croton oil-induced perianal edema in rats for local effects,and xylene-induced mouse ear edema along with carrageenan-induced rat paw edema for systemic effects.Analgesic efficacy was evaluated via the acetic acid-induced mouse writhing test.A rat hemorrhoidal model was generated by topical glacial acetic acid application.Perianal tissue histopathology and fibrosis were examined by HE and Masson staining.Serum concentrations of TNF-α,IL-6,IL-1β,and IL-18 were quantified by ELISA.Protein expression of NLRP3,ASC,Caspase-1,and IL-1β in tissues was analyzed by Western blot.Results:The ointment suppressed inflammatory swelling across all models and attenuated writhing responses in a dose-dependent manner (P<0.01 or P<0.05).In the hemorrhoid model,it markedly reduced inflammatory cell infiltration,tissue edema,and collagen deposition.It also decreased serum levels of pro-inflammatory cytokines and downregulated the expression of NLRP3 inflammasome pathway key proteins (P<0.01 or P<0.05).Conclusion:Zhichuang’an ointment demonstrates significant anti-inflammatory and analgesic effects,potentially mediated through the inhibition of NLRP3 inflammasome activation,leading to reduced release of pro-inflammatory cytokines and amelioration of tissue fibrosi.

参考文献/References:

[1]GUREL E,USTUNOVA S,ERGIN B,et al.Herbal haemorrhoidal cream for haemorrhoids[J].Chin J Physiol,2013,56(5):253-262.
[2]黎爽,彭明沙,李剑,等.珍龙生肌散联合痔炎冲洗灵治疗肛周脓肿疗效研究[J].陕西中医,2022,43(12):1753-1756.
[3]LACAVE-LAPALUN J,BENDERITTER M,LINARD C.Flagellin and LPS each restores rat lymphocyte populations after colorectal irradiation[J].J Leukoc Biol,2014,95(6):931-940.
[4]王志伟,管仲安.基于数据挖掘和网络药理学的痔疮外用中药复方组方规律及机制研究[J].中医临床研究,2024,16(36):80-85.
[5]张艺嘉,余清华,王宇,等.菝葜通过NLRP3通路对巴豆油诱发痔疮大鼠模型的改善作用[J].湖北中医药大学学报,2024,26(2):10-14.
[6]FUJITA T,KARAKI S,TATEOKA T,et al.Desacetyl bisacodyl-induced epithelial Cl(-) secretion in rat colon and rectum[J].Biomed Res,2016,37(1):13-20.
[7]TURNER K,PICKERING M,O’CONNELL P R,et al.The projection of anorectal afferents to cortex of the rat:Comparison of two methods of cortical mapping[J].Neurogastroenterol and Motil,2019,31(12):e13709.
[8]DEY Y N,WANJARI M M,KUMAR D,et al.Curative effect of Amorphophallus paeoniifolius tuber on experimental hemorrhoids in rats[J].J Ethnopharmacol,2016,192:183-191.
[9]PATA F,SGRO A,FERRARA F,et al.Anatomy,physiology and pathophysiology of haemorrhoids[J].Rev Recent Clin Trials,2021,16(1):75-80.
[10]蔡素珠,陈华阳.中药湿敷和中药熏洗坐浴对痔疮术后患者创面愈合及疼痛的影响[J].中国医药指南,2024,22(35):145-147.
[11]胡建文,冯群虎,王业皇,等.王氏生肌散对肛周脓肿大鼠创面愈合及HIF-1α/VEGF信号通路的影响[J].陕西中医,2023,44(9):1179-1183.
[12]张月月,段雅倩,宋玲玲,等.基于抗炎镇痛药效探讨绿豆药汁制黄药子的炮制增效作用[J].时珍国医国药,2025,36(12):2262-2266.
[13]熊翱,魏小雨,彭艳,等.小肠过表达Ski蛋白对扭体小鼠的抑炎镇痛作用[J].中国实验动物学报,2024,32(3):369-377.
[14]GALVAO F H F.Microsurgical technique and results of anorectal transplantation in the rat[J].Transplant Proc,2024,56(5):1138-1140.
[15]张琛伟,许琼明,高红伟,等.白头翁皂苷B4栓剂治疗大鼠痔疮的药效学研究[J].药物评价研究,2022,45(5):853-863.
[16]陈橙,邓宇,龚友兰,等.中医经典名方槐花散的现代研究进展[J].中国临床药理学与治疗学,2023,28(6):697-704.
[17]孙佳婷,吴庆林,佟苗苗,等.地榆HPLC指纹图谱的构建及其COX-2抑制作用谱效关系研究[J].中国医院药学杂志,2025,45(18):2057-2063.
[18]王启,王华,童蕾,等.便血方改善内痔患者临床症状的相关机制研究[J].中华全科医学,2024,22(7):1227-1229.
[19]刘苗苗,李忠原,丰茂秀,等.无花果叶提取物对痔疮及炎症动物模型作用效果[J].中华中医药杂志,2021,36(3):1667-1670.
[20]丛樾,王霄鹏,陈晓彤,等.太宁乳膏对痔切除术后创面愈合的影响[J].中国现代手术学杂志,2006，11(6):411-412.
[21]张馨月,于倩,李胜楠,等.加味三黄膏治疗大鼠肛门溃疡和肿胀的药效机制研究[J].中国药房,2023,34(18):2192-2197.
[22]赵媛,万凯华,翁幼武,等.紫草三黄栓抗炎、消肿、镇痛药效作用的研究[J].新疆医科大学学报,2018,41(7):883-885.
[23]管咏梅,张煜薇,陶颖,等.参柏温敏凝胶的制备及其对痔疮模型大鼠的药效作用[J].中草药,2024,55(23):7999-8010.
[24]YU Q,ZHAO Y,ZHANG X,et al.The beneficial effect of Sanhuang ointment and its active constituents on experimental hemorrhoids in rats[J].J Ethnopharmacol,2024,319(2):117173.
[25]王桂宁,彭涛,张超,等.基于分子对接技术分析三黄汤治疗湿热下注型痔疮的机制[J].安徽医药,2025,29(2):405-408.
[26]WANG H,WANG L,ZENG X,et al.Inflammatory bowel disease and risk for hemorrhoids:A mendelian randomization analysis[J].Scientific Reports,2024,14(1):16677.
[27]龚小慧,谢昌营,罗诗雨,等.基于数据挖掘分析旴江医家治疗痔疮用药规律[J].光明中医,2025，41(1):8-11.
[28]罗小媚,文丹静,陈宇滢,等.中药封包结合护理干预对痔疮术后尿潴留的影响[J].中国城乡企业卫生,2025,40(1):65-67.
[29]KE M,HUANG S,LIN H,et al.Establishment and study of a rat internal haemorrhoid model[J].Sci Rep,2023,13(1):21385.
[30]李宝福,刘柏麟,袁兴,等.参芍口服液抑制NLRP3炎症小体改善创伤性脑损伤小鼠炎性反应实验研究[J].陕西医学杂志,2022,51(5):552-555.

相似文献/References:

[1]姜 帅.黄芪多糖对游离脂肪酸所致人血管内皮细胞NLRP3炎性小体表达的影响[J].陕西中医,2019,(9):1165.

备注/Memo

备注/Memo:
陕西省科技厅秦创源“科学家+工程师”队伍建设项目(2024QCY-KXJ-062)；陕西省重点研发计划项目(2022SF-435)；陕西省咸阳市科技领军人才项目（L2022CXNLRC013）；陕西省高水平学科中药药理学重点学科建设项目（陕中医校字〔2023〕128号）；陕西省中管局中医药科研创新人才项目（TZKN-CXRC-02)
更新日期/Last Update: 2026-04-05