[1]郑彦龙,陈浩琛,赵男,等.百合多糖靶向PI3K/Akt通路干预哮喘气道炎症机制研究[J].陕西中医,2026,(4):455-460.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.004]
　ZHENG Yanlong,CHEN Haochen,ZHAO Nan,et al.The mechanism of Lilium polysaccharide targeting the PI3K/Akt pathway to intervene in airway inflammation in asthma[J].,2026,(4):455-460.[doi:DOI:10.3969/j.issn.1000-7369.2026.04.004]
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百合多糖靶向PI3K/Akt通路干预哮喘气道炎症机制研究

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2026年4期
页码:
455-460
栏目:
基础研究
出版日期:
2026-04-05

文章信息/Info

Title:
The mechanism of Lilium polysaccharide targeting the PI3K/Akt pathway to intervene in airway inflammation in asthma
作者:
郑彦龙1; 陈浩琛1; 赵男1; 王红霞2
（1.河北中医药大学研究生学院，河北 石家庄 050000；2.河北中医药大学第四附属医院肺病科，河北 石家庄 050000）
Author(s):
ZHENG Yanlong1; CHEN Haochen1; ZHAO Nan1; WANG Hongxia2
（1.Graduate School,Hebei University of Chinese Medicine,Shijiazhuang 050000,China；2.Department of Pulmonary Disease,the Fourth Affiliated Hospital of Hebei University of Chinese Medicine,Shijiazhuang 050000,China）
关键词:
支气管哮喘; 百合多糖; 炎症; 核因子κB; 磷脂酰肌醇3-激酶/蛋白激酶B通路
Keywords:
Bronchial asthma; Lilium polysaccharide; Inflammation; NF-κB; PI3K/Akt pathway
分类号:
R 562
DOI:
DOI:10.3969/j.issn.1000-7369.2026.04.004
文献标志码:
A
摘要:
目的：探究百合多糖对哮喘大鼠气道炎症的改善作用及机制。方法：建立大鼠哮喘模型，随机分为对照组、模型组、百合多糖低、高剂量（150、300 mg/kg）组和地塞米松（1 mg/kg）组，每组12只。灌胃2周后，观察哮喘行为并评分；吉姆萨染色检测支气管肺泡灌洗液（BALF）中的炎症细胞数；ELISA法检测血清免疫球蛋白E（IgE）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）水平；HE染色和PAS染色观察肺组织病理变化；免疫组化法检测肺组织Toll样受体4（TLR4）蛋白表达；Western blot检测肺组织磷脂酰肌醇3-激酶/蛋白激酶B通路（PI3K/Akt）通路和核因子κB p65（NF-κB p65）活化情况。结果：与对照组比较，模型组大鼠哮喘症状评分，BALF中炎症细胞数量，血清IgE、IL-6、TNF-α水平升高（均P<0.05）；肺组织呈现典型哮喘病理改变，且炎症及PAS评分、TLR4、p-NF-κB p65/NF-κB p65、p-PI3K/PI3K、p-Akt/Akt蛋白水平升高（均P<0.05）。与模型组比较，百合多糖低、高剂量组和地塞米松组大鼠哮喘症状评分，BALF中炎症细胞数量，血清IgE、IL-6、TNF-α水平降低（均P<0.05）；肺组织哮喘病理改变程度均减轻，且炎症及PAS评分、TLR4、p-NF-κB p65/NF-κB p65、p-PI3K/PI3K、p-Akt/Akt蛋白水平降低（均P<0.05）；且百合多糖的作用呈剂量依赖性（均P<0.05）。结论：百合多糖可减轻哮喘大鼠气道炎症及黏液高分泌，改善哮喘症状，其机制可能与抑制PI3K/Akt/NF-κB信号通路有关。
Abstract:
Objective:To investigate the ameliorative effect and underlying mechanism of Lilium polysaccharide on airway inflammation in asthmatic rats.Methods:A rat asthma model was established and randomly divided into a control group,a model group,low- and high-dose Lilium polysaccharide groups (150,300 mg/kg),and a dexamethasone group (1 mg/kg),with 12 rats in each group.Drugs were administered by gavage for 2 weeks.Asthma-like behaviors were observed and scored.Inflammatory cells in BALF were counted with Giemsa staining.Serum IgE,IL-6 and TNF-α were measured by ELISA.Lung histopathology was evaluated with HE and PAS staining.TLR4 protein expression was detected by immunohistochemistry.And activation of PI3K/Akt pathway and NF-κB p65 was determined by Western blot.Results:Compared with the control group,in the model group,the asthma symptom score of rats,the number of inflammatory cells in bronchoalveolar lavage fluid (BALF),and the levels of serum IgE,IL-6,and TNF-α were increased (all P<0.05).The lung tissue showed typical pathological changes of asthma,and the inflammation and PAS scores,as well as the protein levels of TLR4,p-NF-κB p65/NF-κB p65,p-PI3K/PI3K,and p-Akt/Akt were increased (all P<0.05).Compared with the model group,the asthma symptom scores of rats in the low-and high-dose groups of Lilium polysaccharide and the dexamethasone group,the number of inflammatory cells in BALF,and the levels of serum IgE,IL-6,and TNF-α were decreased (all P<0.05).The degree of pathological changes of asthma in lung tissue was alleviated,and the inflammation and PAS scores,and the protein levels of TLR4,p-NF-κB p65/NF-κB p65,p-PI3K/PI3K and p-Akt/Akt were decreased (all P<0.05).Moreover,the effects of Lilium polysaccharide were dose-dependent (all P<0.05).Conclusion:Lilium polysaccharide can attenuate airway inflammation and mucus hyper-secretion,improve asthmatic symptoms and relieve lung pathological injury in asthmatic rats,possibly by inhibiting the PI3K/Akt/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
河北省中医药管理局科研计划项目（2020153）
更新日期/Last Update: 2026-04-05