[1]鱼清雅,郭从嘉,毕 进,等.正交试验法优选海南黎药冰糖草茎叶中总黄酮成分的提取工艺*[J].陕西中医,2019,(1):131-134.
　YU Qingya,GUO Congjia,BI Jin,et al.Optimum selection of the extraction technology of total flavoniods in the leaves and stems of Hainan Li folk medicine scoparia dulcis L．by orthogonal test[J].,2019,(1):131-134.
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正交试验法优选海南黎药冰糖草茎叶中总黄酮成分的提取工艺*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年1期
页码:
131-134
栏目:
方药纵横
出版日期:
2019-01-05

文章信息/Info

Title:
Optimum selection of the extraction technology of total flavoniods in the leaves and stems of Hainan Li folk medicine scoparia dulcis L．by orthogonal test
文章编号:
DOI:10.3969/j.issn.10007369.2019.01.042
作者:
鱼清雅; 郭从嘉; 毕 进; 张思程; 符亮明; 侯 杰; 陈丽珍
海南医学院药学院（海口 571199）
Author(s):
YU Qingya;  GUO Congjia; BI Jin; et al.
School of Pharmaceutical Science, Hainan Medical College （Haikou 571199)
关键词:
海南黎药; 冰糖草; 总黄酮; 正交试验; 提取工艺
Keywords:
Key words Hainan Li Folk medicine; Scoparia dulcis L; Total flavonoids; Orthogonal test; Extraction technology
分类号:
R963
文献标志码:
A
摘要:
摘 要 目的：优选了海南黎药冰糖草茎叶中总黄酮成分提取工艺。方法：采用L16（45）正交试验，以冰糖草茎叶中总黄酮得率为考察指标，考察乙醇浓度、超声时间、超声温度、超声功率和液料比5个因素对总黄酮得率的影响。结果：海南黎药冰糖草茎叶中总黄酮提取的最佳工艺为80 %乙醇浓度、80 min超声时间、50 ℃超声温度、250 W超声功率、30∶1 ml/g液料比，此条件下总黄酮得率达25.36 mg/g。结论：应用该工艺提取黎药冰糖草茎叶中总黄酮，总黄酮得率较高，方便可行，为黎药冰糖草中总黄酮提取工艺的确定提供实验依据。
Abstract:
Abstract Objection：To optimize the extraction technology of total flavoniods in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L．Methods：By orthogonal design L16(45)，we changed the ethanol concentration，ultrasonic time，ultrasonic temperature，ultrasonic power and ratio of liquid tosolid in order to obtain highest extraction rate of total flavonoids．Results:The best technological parameters for extraction of total flavonoids in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L．are as follows：ethanol concentration of 80 %，ultrasonic time of 80 min，ultrasonic temperature of 50 ℃，ultrasonic power of 250 W，liquid solid ratio of 30:1 ml/g. Under such condition, the yields of total flavonoids were 25.36 mg/g. With high extraction yields of total flavonoids, this technologogy is simple and feasible．Conclusion:This study provides experimental basis for extraction of total flavonoids from in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.

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备注/Memo

备注/Memo:
*国家级大学生创新创业创新训练计划项目（201711810028）; 海南医学院大学生创新训练项目（HYXC2016030）; 海南医学院科研培育基金（HY20152） 
更新日期/Last Update: 2019-03-13