[1]杨 洁,范引科,宋延平,等.扶脾化瘤饮诱导胃癌小鼠细胞凋亡和相关凋亡基因 表达的机制研究[J].陕西中医,2019,(5):550-552，590.
　YANG Jie,FAN Yinke,SONG Yanping,et al.Mechanism of apoptosis and related apoptosis genes Bax and Bcl-2 induced by Fupi Hualiu decoction in MFC gastric cancer mice[J].,2019,(5):550-552，590.
点击复制

扶脾化瘤饮诱导胃癌小鼠细胞凋亡和相关凋亡基因 表达的机制研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年5期
页码:
550-552，590
栏目:
基础研究
出版日期:
2019-05-05

文章信息/Info

Title:
Mechanism of apoptosis and related apoptosis genes Bax and Bcl-2 induced by Fupi Hualiu decoction in MFC gastric cancer mice
文章编号:
DOI:〖HT5K〗10.3969/j.issn.1000-7369.2019.05.002
作者:
杨 洁; 范引科;  宋延平; 李 晔
陕西省中医药研究院（西安 710003）
Author(s):
YANG Jie;  FAN Yinke; SONG Yanping; et al.
Shaanxi Research Institute of Traditional Chinese Medicine (Xi'an 710003)
关键词:
细胞凋亡; 扶脾化瘤饮; 小鼠胃癌细胞; Bax; Bcl-2; 凋亡基因
Keywords:
Apoptosis;  Fupi Hualiu decoction;  MFC;  Bax;  Bcl-2;  Apoptosis genes
分类号:
R285
文献标志码:
A
摘要:
摘 要 目的:研究中药复方扶脾化瘤饮诱导MFC胃癌小鼠细胞凋亡的作用及其对肿瘤组织中Bcl-2及Bax蛋白表达的影响,初步探讨其可能的抑瘤机制。方法:建立胃癌(MFC)小鼠动物模型,并将模型动物随机分为模型对照组、扶脾化瘤饮高、中、低剂量组共4组。扶脾化瘤饮高、中、低剂量组给药剂量分别为 6.0、3.0、1.5 g/kg ，模型组给予等体积纯净水。连续给药、给水 14 d 处死小鼠，分离肿瘤称重计算抑瘤率,部分采用流式细胞仪检测胃癌细胞凋亡率变化；部分石蜡包埋，肿瘤组织切片，免疫组织化学法检测 Bax、Bcl-2 蛋白表达。结果:经扶脾化瘤饮作用后,小鼠胃癌细胞凋亡率明显增加,且表现出良好的剂量关系。扶脾化瘤饮高、中剂量组显著上调肿瘤组织 Bax 蛋白表达，下调 Bcl-2 蛋白表达，与模型对照组比较，差异有统计学意义(P<0.05，P<0.01)。结论:扶脾化瘤饮可能通过下调Bcl-2表达，上调Bax表达，诱导肿瘤细胞凋亡,达到抗肿瘤治疗的目的。
Abstract:
Abstract Objective: To study the effect of Chinese herbal compound Fupi Hualiu decoction on apoptosis of MFC gastric cancer mice and its effect on the expression of Bcl-2 and Bax protein in tumor tissues, and to explore its possible anti-tumor mechanism. Methods: Animal models of gastric cancer (MFC) mice were established, and the model animals were randomly divided into model control group and Fupi Hualiu decoction high, medium and low dose groups. The doses of the high, medium and low doses of Fupi Hualiu decoction were 6.0g/kg, 3.0g/kg, 1.5g/kg, respectively, and the model group was given an equal volume of purified water. The mice were sacrificed by continuous administration and water supply for 14 days. The tumor inhibition rate was calculated by tumor weighing. The apoptosis rate of gastric cancer cells was detected by flow cytometry. Some paraffin-embedded, tumor tissue sections and immunohistochemical method were used to detect Bax and Bcl-2 protein expression. Results: After the treatment of Fupi Hualiu decoction , the apoptotic rate of gastric cancer cells increased significantly and showed a good dose relationship. The high and middle dose groups of Fupi Hualiu decoction significantly up-regulated the expression of Bax protein in tumor tissue and down-regulated the expression of Bcl-2 protein, which was statistically significant compared with the model control group (P<0.05, P<0.01). Conclusion: Fupi Hualiu decoction may induce the apoptosis of tumor cells by down-regulating the expression of Bcl-2 and up-regulating the expression of Bax, thus achieving the goal of anti-tumor therapy.

参考文献/References:

[1] 陈浩洋，郑永绵，刘明辉.Gab2基因在胃癌中的表达及对胃癌细胞增殖、凋亡、迁移及AKT、p-AKT、Bax、Bcl-2表达的影响研究 [J].癌症进展，2017，15（3）：262-266.
[2] 屈小元，杨成祖．扶脾化瘤饮治疗脾胃虚弱型中晚期胃癌32例［J］．陕西中医，2010，31（1）：9-10．
[3] 赵雅宁，王红阳，李 琳.不同程度间歇性低氧对大鼠不同脑区Bax、Bcl-2表达的影响 [J].中国老年学杂志，2015，35（1）：168-169.
[4] 赵羲和，李 凯，邹华伟.Bax介导硼替佐米诱导胃癌细胞株MKN45凋亡作用的研究 [J] 。中国现代医学杂志，2017，27（1）：11-15.
[5] 何莉莎，李枋霏，孙桂芝，等．中药诱导肿瘤细胞凋亡的实验研究进展［J］．河北中医，2014，36(2) : 309-312．
[6] 焦 宁，陈 冲，徐瑞荣.益气养阴方及其拆方对急性髓细胞白血病小鼠细胞凋亡及相关蛋白的影响［J］．中国中医药信息杂志，2014，21（2）：42-45.
[7] 王丽萍，曾宪旭，牛凤兰,等. 五倍子酸对小鼠胃癌MFC和肝癌H22细胞增殖的抑制作用［J］. 郑州大学学报：医学版,2012,47(3):339-341.
[8] 王 金，王 芳，王小平，等.藤珠胃康颗粒含药血清对小鼠胃癌MFC 细胞增殖和凋亡的影响［J］.广东药学院学报，2016，32(6)：733-736.
[9] 许 晶，侯 丽，武 苗，等.新加良附方联合5-FU对裸鼠胃癌移植瘤Bax/Bcl-2表达的影响 [J].世界中西医结合杂志，2013,8（7）:675-678.
[10] 蒋时红，刘旺根，谢慧珺，等.3种治法方药诱导H22荷瘤小鼠细胞凋亡的实验研究 [J].中国实验方剂学杂志,2009,15（12）:78-80.

相似文献/References:

[1]朱军义,马繁华,徐亚辉.龙葵碱调控p-AKT、Cleaved caspase-3和p53蛋白表达抑制卵巢癌 细胞增殖实验研究*[J].陕西中医,2019,(5):556.
　ZHU Junyi,MA Fanhua,XU Yahui..Solanum nigrum inhibits proliferation and promote apoptosis of ovarian cancer cells through regulates the expression of p-AKT, Cleaved caspase-3 and p53 protein[J].,2019,(5):556.
[2]张贺,姜大庆.鸦胆子素D对乳腺癌MDAMB231细胞增殖与凋亡及细胞周期的影响*[J].陕西中医,2019,(11):1495.
　ZHANG He,JIANG Daqing..Effects of Bruceine D on proliferation,apoptosis and cell cycle of breast cancer MDAMB231 cells[J].,2019,(5):1495.

备注/Memo

备注/Memo:
*陕西省中医药管理局科研项目(JCPT041)
更新日期/Last Update: 2019-05-07