[1]张贺,姜大庆.鸦胆子素D对乳腺癌MDAMB231细胞增殖与凋亡及细胞周期的影响*[J].陕西中医,2019,(11):1495-1497.
　ZHANG He,JIANG Daqing..Effects of Bruceine D on proliferation,apoptosis and cell cycle of breast cancer MDAMB231 cells[J].,2019,(11):1495-1497.
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鸦胆子素D对乳腺癌MDAMB231细胞增殖与凋亡及细胞周期的影响*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2019年11期
页码:
1495-1497
栏目:
基础研究
出版日期:
2019-11-05

文章信息/Info

Title:
Effects of Bruceine D on proliferation,apoptosis and cell cycle of breast cancer MDAMB231 cells
文章编号:
DOI:10.3969/j.issn.10007369.2019.11.002
作者:
张贺1; 姜大庆2
1.辽宁中医药大学第一临床学院2017级硕士研究生 （沈阳 110167）；2.辽宁省肿瘤医院乳腺外科（沈阳 110042）
Author(s):
ZHANG He; JIANG Daqing.
Master Degree Candidate in 2017,First Clinical Medical College of Liaoning University of Traditional Chinese Medicine(Shenyang 110167)
关键词:
乳腺癌; 鸦胆子素D; 细胞增殖; 细胞凋亡; 细胞周期
Keywords:
Key words Breast cancer; Bruceine D; Cell proliferation; Apoptosis; The cell cycle
分类号:
R737.9
文献标志码:
A
摘要:
摘要目的：探讨鸦胆子素D对人乳腺癌细胞株MDAMB231增殖、凋亡及细胞周期的影响。方法： MDAMB231细胞加入不同浓度的鸦胆子素D（0、3、5、10、30、50 μmol/L）分别作用24 h、48 h、72 h后，CCK8检测细胞增殖情况。流式细胞仪检测细胞凋亡及周期情况。结果： CCK8结果提示经不同浓度鸦胆子素D作用后，MDAMB231细胞存活率下降，与对照组相比差异有统计学意义(P＜005)。细胞凋亡结果提示经0、10、30、50 μmol/L的鸦胆子素D作用24 h后，总细胞群中凋亡细胞百分比分别为6.69%、15.12%、35.83%和40.67%，与对照组差异有统计学意义(P＜005)。细胞周期结果提示经0、10、30、50 μmol/L的鸦胆子素D作用24 h后，G0/G1期乳腺癌细胞占比分别为31.45%、40.93%、 57.16%、 66.38%；S期乳腺癌细胞占比分别为40.12%、33.08%、29.32%、20.45%；G2/M期乳腺癌细胞占比分别为28.43%、25.99%、13.52%、11.17%,与对照组相比差异有统计学意义(P＜005)。结论： MDAMB231细胞存活率随鸦胆子素D药物浓度增加而降低，且时间越长细胞存活率越低，呈时间剂量依赖性。鸦胆子素D促进MDAMB231细胞凋亡比例增加且其增殖周期阻滞在C0/G1期。
Abstract:
AbstractObjective:Investigate the effects of Bruceine D on proliferation,apoptosis and cell cycle of human breast cancer cell line MDAMB231.Methods:CCK8 was used to detect the proliferation of Bruceine D (0,3,5,10,30,50) μmol/L MDAMB231 cell after 24 h,48 h,72 h,respectively.Apoptosis and cell cycle were detected by flow cytometry.Results:CCK8 suggested that after Bruceine D at different concentrations,the survival rate of MDAMB231 cells decreased,and the difference was statistically significant compared with the control group (P＜005).The results of apoptosis suggested that the percentage of apoptotic cells in the total cell population was 6.69%,15.12%,35.83% and 40.67%,respectively,after 24 h of Bruceine D treatment with 0,10,30 and 50 μmol/L,which was statistically significant different from the control group (P＜005).The cell cycle results suggested that after 24 h of Bruceine D treatment with 0,10,30 and 50 μmol/L,the proportion of G0/G1 breast cancer cells was 31.45%,40.93%,57.16% and 66.38%;the proportion of S breast cancer cells was 40.12%、33.08%、29.32% and 20.45%;the proportion of G2/M breast cancer cells was 28.43%、25.99%、13.52% and 11.17%,respectively,and the difference was statistically significant compared with the control group (P＜005).Conclusion:The survival rate of MDAMB231 cells decreases with the increase of Bruceine D concentration,and the cell survival rate decreases with the increase of time.Bruceine D increased the apoptosis rate of MDAMB231 cells and its proliferation cycle is blocked at C0/G1 phase.

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备注/Memo

备注/Memo:
*沈阳市科技局资助项目（F10149961）
更新日期/Last Update: 2019-11-15