[1]张 伟,林 海△,常 翔,等.柔筋止颤片通过抑制ROS通路减轻MPP+诱导SH-SY5Y 细胞凋亡实验研究*[J].陕西中医,2020,(2):160-163.
　ZHANG Wei,LIN Hai,CHANG Xiang,et al.Roujin Zhichan tablets attenuates MPP+induced SH-SY5Y cell apoptosis by inhibiting ROS pathway[J].,2020,(2):160-163.
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柔筋止颤片通过抑制ROS通路减轻MPP+诱导SH-SY5Y 细胞凋亡实验研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年2期
页码:
160-163
栏目:
基础研究
出版日期:
2020-02-05

文章信息/Info

Title:
Roujin Zhichan tablets attenuates MPP+induced SH-SY5Y cell apoptosis by inhibiting ROS pathway
文章编号:
DOI:〖HT5K〗10.3969/j.issn.10007369.2020.02.006
作者:
张 伟; 林 海△; 常 翔; 杨 琳; 吕富荣; 左 瑞; 田 兵
西安市中医医院脑病科(西安 710021)
Author(s):
ZHANG Wei; LIN Hai; CHANG Xiang; et al.
Department of Encephalopathy,Xi'an Traditional Chinese Medicine Hospital(Xi'an 710021)
关键词:
柔筋止颤片;  帕金森病;  SH-SY5Y 细胞;  细胞凋亡;  实验研究
Keywords:
Roujin Zhichan tablets;  Parkinson's disease;  SH-SY5Y cell;  Cell apoptosis;  Experimental study
分类号:
R742
文献标志码:
A
摘要:
目的:研究柔筋止颤片保护由MPP+诱导的SH-SY5Y 细胞损伤的影响及其机制。方法: MTT检测细胞活性,DHE检测O2-,DCFH-DA检测ROS,Western blot检测Caspases蛋白表达水平。结果:SH-SY5Y细胞经MPP+(500 μmol/L)处理24 h后细胞活性降低(P<0.05),O2-和ROS水平均升高(P<0.05),Clevaed Caspase-3、Clevaed Caspase-8和Clevaed Caspase-9蛋白水平均增高(P<0.05)。柔筋止颤片(25 nmol/L)预处理24 h可以显著维持细胞活性降低(P<0.05),降低O2-和ROS的水平(P<0.05),Clevaed Caspase-3、Clevaed Caspase-8和Clevaed Caspase-9蛋白水平下降(P<0.05)。结论: 体外实验证实柔筋止颤片可减少MPP+诱导的O2-和ROS水平升高以减少线粒体的损伤,并减少Caspases蛋白的活化以抑制细胞凋亡程序的启动,进而保护MPP+诱导的SH-SY5Y细胞活性降低,为进一步优化柔筋止颤片的研究提供了实验依据。
Abstract:
Objective:This study aims to investigate the protecting mechanism of Roujin Zhichan tablets in MPP+ induced SH-SY5Y cells.Methods:MTT assay was used to analyze the cell activity,DHE assay was used to analyze the level of O2-,DCFH-DA assay was used to analyze the level of ROS,and western blot assay were used to detect the protein level of Caspases.Results:Compared with the control group,SH-SY5Y cells treated by MPP+(500 μmol/L)for 24 h showed a decrease in cell activity(P<0.05),an increase in the level of O2- and ROS(P<0.05),and an increase in the protein level of Clevaed Caspase-3,Clevaed Caspase-8 and Cleved Caspase-9 proteins(P<0.05).Roujin Zhichan tablets(25 nmol/L)pretreatment for 24 h significantly maintained the cell activity(P<0.05),decreased the level of O2- and ROS(P<0.05),and decreased the protein level of Clevaed Caspase-3,Clevaed Caspase-8 and Clevaed Caspase-9(P<0.05).Conclusion:The vitro experiments showed that Roujin Zhichan tablets can reduce MPP+ induced elevated the level of O2- and ROS to reduce the mitochondrial damage,and reduce the level of Clevaed-Caspases to inhibit the initiation of apoptotic process,thereby protecting SH-SY5Y cells from MPP+ induced decreased activity.It provides experimental basis for further optimization of Roujin Zhichan tablets research.

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备注/Memo

备注/Memo:
*陕西省中医药管理局课题(ZYPT009)
更新日期/Last Update: 2020-03-31