[1]梁应凤,郭姗姗,张 丹,等.健脾益肾方对非小细胞肺癌细胞体外增殖凋亡作用的实验研究*[J].陕西中医,2020,(5):574-577.[doi:DOI:10.3969/j.issn.10007369.2020.05.006]
　LIANG Yingfeng,GUO Shanshan,ZHANG Dan,et al.Effect of Jianpi Yishen prescription on proliferation and apoptosis of non-small cell lung cancer cells in vitro[J].,2020,(5):574-577.[doi:DOI:10.3969/j.issn.10007369.2020.05.006]
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健脾益肾方对非小细胞肺癌细胞体外增殖凋亡作用的实验研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年5期
页码:
574-577
栏目:
基础研究
出版日期:
2020-05-05

文章信息/Info

Title:
Effect of Jianpi Yishen prescription on proliferation and apoptosis of non-small cell lung cancer cells in vitro
作者:
梁应凤1; 郭姗姗1; 张 丹1; 卢敬钗1; 叶 奇; 高 燕1; 李 倩1△; Latimer N2
1.解放军火箭军特色医学中心(北京 100088); 2.英国谢菲尔德大学健康与健康研究学院卫生经济学和决策科学(谢菲尔德市 S1 4DA)
Author(s):
LIANG Yingfeng; GUO Shanshan; ZHANG Dan; et al.
The PLA Rocket Force Characteristic Medical Center(Beijing 100088)
关键词:
健脾益肾方;  非小细胞肺癌;  凋亡;  增殖;  Survivin;  Bcl-2;  Caspase-3;  体外实验
Keywords:
Jianpi Yishen prescription;  Non-small cell lung cancer;  Apoptosis;  Proliferation;  Survivin;  Bcl-2;  Caspase-3;  In vitro test
分类号:
R734.2
DOI:
DOI:10.3969/j.issn.10007369.2020.05.006
文献标志码:
A
摘要:
目的:探讨健脾益肾方对非小细胞肺癌(NSCLC)细胞体外增殖凋亡的作用。方法:人NSCLC细胞系A549分为四组:空白对照组(仅加入细胞培养液)、阴性对照组(加入细胞,不进行中药处理)、实验组(加细胞加中药处理)。荧光定量PCR和Western blot分别检测Survivin、Bcl-2和Caspase-3的mRNA和蛋白表达。MTT检测细胞增殖; 流式细胞术检测细胞凋亡。结果:与空白对照组相比,阴性对照组细胞在24、48、72 h的吸光度值明显升高,细胞凋亡率下降,Survivin和Bcl-2 mRNA和蛋白相对表达量上调,Caspase-3 mRNA和蛋白相对表达量下调,组间比较差异有统计学意义(P<0.05); 而健脾益肾方处理的实验组24、48、72 h的吸光度值均显著降低,细胞凋亡率显著上升,Survivin和Bcl-2 mRNA和蛋白相对表达量下调,Caspase-3 mRNA和蛋白相对表达量上调,与空白对照组相比差异有统计学意义(P<0.05)。结论:健脾益肾方可通过下调Survivin和Bcl-2、上调Caspase-3表达诱导NSCLC细胞凋亡,并抑制肿瘤细胞的增殖,进而抑制NSCLC的发展。
Abstract:
Objective:To study the effect of Jianpi Yishen prescription on the proliferation and apoptosis of non-small cell lung cancer(NSCLC)cells in vitro.Methods:Human NSCLC cell line A549 was divided into four groups:blank control group(only adding cell culture medium),negative control group(adding cells,without Chinese medicine treatment),experimental group(adding cells and Chinese medicine treatment).Furthermore,mRNA and protein expression of Survivin and Caspase-3 were detected by fluorescence quantitative PCR and Western blot,respectively.Cell proliferation was detected by MTT and apoptosis was detected by flow cytometry.Results:Compared with the blank control group,the absorbance value of the negative control group increased significantly at 24 h,48 h and 72 h,the apoptosis rate decreased,the relative expression of Survivin and Bcl-2 mRNA and protein increased,while those of caspase-3 mRNA and protein decreased,and the differences between the groups were significant(P<0.05); while the absorbance value of the experimental group treated with Jianpi Yishen prescription decreased significantly at 24 h,48 h and 72 h,accompanied by increased apoptosis rate,down-regulated mRNA and protein expression of Survivin and Bcl-2,and up-regulated caspase-3 mRNA and protein expression,showing statistically significant difference when compared with the blank control group(P<0.05).Conclusion:The Jianpi Yishen prescription can induce NSCLC cell apoptosis by down-regulating the expression of Survivin and Bcl-2 as well as up-regulating Caspase-3 expression,inhibit the proliferation of tumor cells,and may thus inhibit the development of NSCLC.

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备注/Memo

备注/Memo:
*军队医学科技青年培育计划项目(19QNP034)
更新日期/Last Update: 2020-05-14