[1]丁 维,钟立仁,张海波,等.丹参酮ⅡA磺酸钠对高脂血症勃起功能障碍大鼠Nrf2/HO-1通路及勃起功能的影响*[J].陕西中医,2020,(11):1520-1523,1538.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.002]
　DING Wei,ZHONG Liren,ZHANG Haibo,et al.Sodium tanshinone ⅡA sulfonate ameliorates erectile function in hyperlipidemia ED rats by up-regulating Nrf2/HO-1[J].,2020,(11):1520-1523,1538.[doi:DOI:10.3969/j.issn.1000-7369.2020.11.002]
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丹参酮ⅡA磺酸钠对高脂血症勃起功能障碍大鼠Nrf2/HO-1通路及勃起功能的影响*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年11期
页码:
1520-1523,1538
栏目:
基础研究
出版日期:
2020-11-05

文章信息/Info

Title:
Sodium tanshinone ⅡA sulfonate ameliorates erectile function in hyperlipidemia ED rats by up-regulating Nrf2/HO-1
作者:
丁 维1; 钟立仁2; 张海波2; 范钧泓2; 王 力2; 何书华2; 王明强3; 韦安阳2△
1.贵州中医药大学附属第一医院泌尿外科(贵阳 550001); 2.南方医科大学南方医院泌尿外科(广州 510515); 3.贵州中医药大学(贵阳 550025)
Author(s):
DING Wei; ZHONG Liren; ZHANG Haibo; et al.
Department of Urology,the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine(Guiyang 550001)
关键词:
高脂血症;  勃起功能障碍;  丹参酮ⅡA磺酸钠;  氧化应激;  大鼠;  丙二醇
Keywords:
Hyperlipidemia;  Erectile dysfunction;  Sodium tanshinone Ⅱ A sulfonate;  Oxidative stress;  Rats;  MDA
分类号:
R589.2
DOI:
DOI:10.3969/j.issn.1000-7369.2020.11.002
文献标志码:
A
摘要:
目的:观察丹参酮ⅡA磺酸钠(STS)对高脂血症(HR)勃起功能障碍(ED)大鼠Nrf2/HO-1通路及对勃起功能的影响。方法:实验共纳入8周龄SD雄性大鼠18只,随机分为正常组、高脂血症组、高脂血症加丹参酮ⅡA磺酸钠组各6只。通过喂养高脂饲料以构建高脂血症大鼠模型,高脂血症加丹参酮ⅡA磺酸钠组每天腹腔注射10 mg/kg的丹参酮ⅡA磺酸钠溶液,高脂血症组每日腹腔注射等量生理盐水。16周后检测大鼠阴茎海绵体内压(ICP)及颈动脉压(MAP),体重及血脂; 使用Western blot分析体内调控抗氧化应激通路的关键蛋白Nrf2和HO-1的表达水平,并检测阴茎海绵体组织内氧化应激水平相关分子丙二醇(MDA)、谷胱甘肽(GSH)和超氧化歧化酶(SOD)的含量。结果:治疗后,高脂血症加丹参酮ⅡA磺酸钠组和高脂血症组体重较正常组明显增加(P<0.05),高脂血症加丹参酮ⅡA磺酸钠组相比高脂血症组甘油三酯降低,高密度脂蛋白增高(P<0.05); 高脂血症加丹参酮ⅡA磺酸钠组ICP/MAP比值高于高脂血症组(P<0.05); 与正常组相比,高脂血症组大鼠阴茎海绵体组织的GSH和SOD水平下降、MDA含量上升,而丹参酮ⅡA磺酸钠组治疗后能明显提高GSH和SOD水平、降低高脂血症组大鼠MDA水平(P<0.05); Western blot结果显示高脂血症组Nrf2和HO-1的表达水平较正常组升高,而丹参酮ⅡA磺酸钠治疗后Nrf2和HO-1的表达进一步升高(P<0.05)。结论:丹参酮ⅡA磺酸钠治疗可通过上调Nrf2/HO-1并抑制氧化应激水平,进而改善高脂血症大鼠的勃起功能。
Abstract:
Objective:To observe the effect of Tanshinone ⅡA sulfonate(STS)on Nrf2/HO-1 pathway and erectile function in hyperlipidemia(HR)ED rats.Methods:A total of 18 8-week-old SD male rats were included in the experiment,and they were randomly divided into 3 groups(n=6 per group):normal control group,hyperlipidemia group,hyperlipidemia with tanshinone ⅡA sodium sulfonate group.The hyperlipidemia rat model was constructed by feeding high-fat diet.The HR with STS group was intraperitoneally injected with 10mg/kg STS solution every day,and the HR group was intraperitoneally injected with the same amount of normal saline every day.After 16 weeks,the rat penile intracavernosal pressure(ICP)and mean carotid artery pressure(MAP),body weight and blood lipids were measured; western blot was used to analyze the expression levels of key proteins Nrf2 and HO-1 that regulate the anti-oxidative stress pathway in vivo,and detect the levels of propylene glycol(MDA),glutathione(GSH)and superoxide dismutase(SOD)in the corpus cavernosum of the penis.Results:After treatment,the body weight of the STS group and the HR group was significantly higher than that of the NC group(P<0.05).Compared with the HR group,the triglycerides of the STS group were significantly lower,and the high-density lipoprotein was significantly increased(P<0.05).The ICP/MAP ratio of HR with STS group was significantly higher than that of the HR group(P<0.05).Compared with the NC group,the GSH and SOD levels of the penile cavernous tissue of the HR group decreased,and the MDA content increased,while STS treatment could significantly increase GSH and SOD.Western-blot results showed that the expression levels of Nrf2 and HO-1 in the HR group were higher than those in the NC group,while the expression levels of Nrf2 and HO-1 were further increased after STS treatment(P<0.05).Conclusion:STS treatment can improve the erectile function of hyperlipidemia rats by up-regulating Nrf2/HO-1 and inhibiting the level of oxidative stress.

参考文献/References:

[1] 李 宁.糖尿病性勃起功能障碍患者性激素检测及临床意义[J].陕西医学杂志,2012,41(7):893-894.
[2] Hwang I,Lee H,Yu HS,et al.Testosterone modulates endothelial progenitor cells in rat corpus cavernosum[J].British International Journal of Urology,2016,117(6):976-981.
[3] Wei M,Macera CA,Davis DR,et al.Total cholesterol and high density lipoprotein cholesterol as important predictors of erectile dysfunction[J].American Journal of Epidemiology,1994(140):930-937.
[4] Furukawa S,Fujita T,Shimabukuro M,et al.Increased oxidative stress in obesity and its impact on metabolic syndrome[J].Journal of Clinical Investigation,2004(114):1752-1761.
[5] 吴庆昕.丹参调节肾组织AQP2效应与其活血利尿功效的相关性研究[J].陕西中医,2018,39(4):419-421.
[6] 曹 珊,许 梅,张湘燕.丹参减轻炎性因子-炎症反应作用探讨[J].现代中西医结合杂志,2019,28(11):1251-1254.
[7] Zhang XD,He CX,Cheng J,et al.Sodium tanshinone Ⅱ-A sulfonate(DS-201)induces vasorelaxation of rat mesenteric arteries via inhibition of L-type Ca2+ channel[J].Frontiers in Pharmacology,2018(9):62.
[8] Wei B,You MG,Ling JJ,et al.Regulation of antioxidant system,lipids and fatty acid beta-oxidation contributes to the cardioprotective effect of sodium tanshinone ⅡA sulphonate in isoproterenol-induced myocardial infarction in rats[J].Atherosclerosis,2013(230):148-156.
[9] Hu C,Dong YY,Dong YH,et al.Identification of oxidative stress-induced gene expression profiles in cavernosal endothelial cells[J].Molecular Medicine Reports,2015(11):2781-2788.
[10] Lv D,Zhou Q,Xia Y,et al.The association between oxidative stress alleviation via sulforaphane-induced Nrf2-HO-1/NQO-1 signaling pathway activation and chronic renal allograft dysfunction improvement[J].Kidney & Blood Pressure Research,2018(43):191-205.
[11] Zhao Z,Liao G,Zhou Q,et al.Sulforaphane attenuates contrast-induced nephropathy in rats via Nrf2/HO-1 pathway[J].Oxidative Medicine and Cellular Longevity,2016(2016):9825623.
[12] Cao W,Shi M,Wu L,et al.Adipocytes initiate an adipose-cerebral-peripheral sympathetic reflex to induce insulin resistance during high-fat feeding[J].Clinical Science,2019(133):1883-1899.
[13] 王琳莉,黄抒伟,窦丽萍,等.丹参酮ⅡA磺酸钠对兔急性心梗再灌注后无复流的保护作用[J].中华中医药学刊,2016,34(3):678-682.
[14] Angulo J,El AM,Sevilleja OA,et al.Short-term pharmacological activation of Nrf2 ameliorates vascular dysfunction in aged rats and in pathological human vasculature,a potential target for therapeutic intervention[J].Redox Biology,2019(26):101271.
[15] Silva RA,Fraga F,Montecucco FM,et al.Pathophysiological role of the renin-angiotensin system on erectile dysfunction[J].Journal of Clinical Investigation,2013(43):978-985.

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备注/Memo

备注/Memo:
*国家自然科学基金资助面上项目(81571433);广东省自然科学基金资助项目(2017A030313453,2018A030313638)
更新日期/Last Update: 2020-11-09