[1]黄 钢,马善波,杨双武,等.鹅不食草介导Nrf2/HO-1信号通路在脑缺血再灌注中抗氧化抗炎作用机制研究*[J].陕西中医,2020,(12):1699-1703.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
　HUANG Gang,MA Shanbo,YANG Shuangwu,et al.Centipeda minima mediated anti-oxidation and anti-inflammatory through Nrf2/HO-1 pathway in cerebral ischemia-reperfusion[J].,2020,(12):1699-1703.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
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鹅不食草介导Nrf2/HO-1信号通路在脑缺血再灌注中抗氧化抗炎作用机制研究*
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2020年12期
页码:
1699-1703
栏目:
基础研究
出版日期:
2020-12-05

文章信息/Info

Title:
Centipeda minima mediated anti-oxidation and anti-inflammatory through Nrf2/HO-1 pathway in cerebral ischemia-reperfusion
作者:
黄 钢1; 马善波2; 杨双武1; 高海锋3△
1.空军军医大学西京医院神经外科(西安 710032); 2.空军军医大学西京医院药剂科(西安 710032); 3.西北大学附属医院(西安市第三医院)(西安 710018)
Author(s):
HUANG Gang; MA Shanbo; YANG Shuangwu; et al.
Department of Neurosurgery,Xijing Hospital,Air Force Medical University(Xi'an 710032)
关键词:
鹅不食草;  氧化应激;  炎症因子;  脑缺血;  Nrf2/HO-1信号通路
Keywords:
Centipeda minima;  Oxidative stress;  Inflammatory factors;  Cerebral ischemia;  Nrf2/HO-1 pathway
分类号:
R281
DOI:
DOI:10.3969/j.issn.1000-7369.2020.12.005
文献标志码:
A
摘要:
目的:探讨鹅不食草对脑缺血再灌注大鼠Nrf2/HO-1信号通路介导的氧化应激反应和神经炎症影响。方法:SD大鼠分为假手术组、脑缺血组、依达拉奉组、鹅不食草100 mg/kg剂量组、鹅不食草200 mg/kg剂量组、鹅不食草400 mg/kg剂量组。采用中动脉栓塞介导大鼠缺血损伤,连续给药后评价大鼠神经功能,脑组织脑梗死面积、氧化因子、炎症因子含量,Nrf2、HO-1 mRNA与蛋白含量。结果:与假手术组相比,脑缺血组大鼠神经功能显著损伤(P<0.05),脑梗死体积增加(P<0.05),氧化因子、炎症因子含量增加(P<0.05)。与脑缺血组相比,鹅不食草组与依达拉奉组改善了大鼠神经功能(P<0.05),减少脑梗死面积(P<0.05),抑制氧化应激与炎症反应(P<0.05),而Nrf2、HO-1 mRNA与蛋白表达增加(P<0.05)。结论:鹅不食草可以介导Nrf2/HO-1信号通路减少大鼠脑中氧化应激反应与炎症因子含量,改善大鼠神经功能。
Abstract:
Objective:To explore the effects of centipeda minima on oxidative stress response and neuroinflammation through Nrf2/HO-1 signaling pathway in rats with cerebral ischemia-reperfusion.Methods:SD rats were randomly divided into sham group,cerebral hypoxia group,edaravone group,centipeda minima 100 mg/kg,200 mg/kg,400 mg/kg.The middle cerebral artery occlusion operations were applied for ischemic injury in rats.The nerve function of rats was evaluated after continuous drug administration.The brain tissue was taken for cerebral infarction detection,oxidative factors,inflammatory factors and the expression of Nrf2 mRNA,HO-1 mRNA and proteins.Results:Compared with sham group,cerebral hypoxia group had significant neurological function damage(P<0.05),increased cerebral infarction area(P<0.05),and elevated levels of oxidative factors and inflammatory factors(P<0.05).Compared with cerebral hypoxia group,centipeda minima and edaravone administration improved nerve function in rats(P<0.05),reduced cerebral infarction(P<0.05),reduced the expression of oxidative factors and inflammatory factors in the brain(P<0.05),increased the expression of Nrf2,HO-1 mRNA and protein(P<0.05).Conclusion:Centipeda minima can improve the nerve function through Nrf2,HO-1 signaling pathway which mediate oxidative stress and inflammation reduction in the brain of rats.

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备注/Memo

备注/Memo:
*空军军医大学西京医院助推课题(XJZT18MJ41)
更新日期/Last Update: 2020-12-11