[1]王 军,徐建良,杜燕红.扶正祛邪汤对肝癌模型大鼠肠道菌群微生态环境的作用研究[J].陕西中医,2021,(2):143-147.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.002]
　WANG Jun,XU Jianliang,DU Yanhong.Effect of Fuzheng Quxie decoction on intestinal microflora microecological environment of liver cancer model rats[J].,2021,(2):143-147.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.002]
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扶正祛邪汤对肝癌模型大鼠肠道菌群微生态环境的作用研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年2期
页码:
143-147
栏目:
基础研究
出版日期:
2021-02-05

文章信息/Info

Title:
Effect of Fuzheng Quxie decoction on intestinal microflora microecological environment of liver cancer model rats
作者:
王 军; 徐建良; 杜燕红
(湖北省中医院,湖北 武汉 430000)
Author(s):
WANG Jun; XU Jianliang; DU Yanhong
(Hubei Hospital of Traditional Chinese Medicine,Wuhan 430000,China)
关键词:
肝癌;  扶正祛邪汤;  肠道菌群微生态环境;  TLR4/NF-κB信号通路;  大鼠
Keywords:
Liver cancer;  Fuzheng Quxie decoction;  Microecological environment of intestinal flora;  TLR4/NF-κB signaling pathway;  Rats
分类号:
R 735.7
DOI:
DOI:10.3969/j.issn.1000-7369.2020.02.002
文献标志码:
A
摘要:
目的:探究扶正祛邪汤对肝癌模型大鼠肠道菌群微生态环境的改善作用。方法:选择96只SD健康雄性大鼠,随机分为正常组、模型组、给药组各32只,模型组和给药组构建肝癌模型建模成功后给药组大鼠采用350 mg/kg的扶正祛邪汤灌胃,模型组、正常组采用同剂量生理盐水灌胃。检测三组大鼠肠道菌群微生态环境,检测三组大鼠甲胎蛋白(AFP)、肿瘤标志物(CA19-9)、谷丙转氨酶(ALT),并检测大鼠TLR4/NF-κB信号通路表达量。结果:与正常组相比,模型组、给药组变形菌门、厚壁菌门、拟杆菌门、放线菌门、CA19-9、AFP、ALT、TLR4/NF-κB信号通路水平均高于正常组,差异具有统计学意义(均P<0.05); 与模型组相比,正常组、给药组变形菌门、厚壁菌门、拟杆菌门、放线菌门、CA19-9、AFP、ALT、TLR4/NF-κB信号通路水平均低于模型组,差异具有统计学意义(均P<0.05); 与给药组相比,正常组变形菌门、厚壁菌门、拟杆菌门、放线菌门、CA19-9、AFP、ALT、TLR4/NF-κB信号通路水平均低于模型组,且模型组变形菌门、厚壁菌门、拟杆菌门、放线菌门、CA19-9、AFP、ALT、TLR4/NF-κB信号通路水平均高于正常组,差异具有统计学意义(均P<0.05)。结论:扶正祛邪汤可能经TLR4/NF-κB信号通路修复肝癌,改善大鼠肠道菌群微生态环境,发挥其肝功能保护作用。
Abstract:
Objective:To explore the effect of Fuzheng Quxie decoction on the microecological environment of intestinal flora in rats with liver cancer model.Methods:96 SD healthy male rats were randomly divided into normal group,model group and drug administration group.After modeling the liver cancer model successfully,350 mg/kg Fuzheng Quxie decoction was used in the model group and normal group.The microecological environment of intestinal flora in three groups of rats was detected,the alpha-fetoprotein(AFP),tumor marker(CA19-9)and alanine aminotransferase(ALT)were detected in three groups of rats,and the expression of TLR4/NF-κB signaling pathway in rats was detected.Results:Compared with the normal group,the levels of proteus,firmicum,bacteroides,actinomycetes,CA19-9,AFP,ALT,TLR4/NF-κB signaling pathway in the model group and the administration group were higher than those in the normal group(all P<0.05); compared with the model group,the levels of proteus,firmicum,bacteroides,actinomycetes,CA19-9,AFP,ALT,TLR4/NF-κB signaling pathway in the model group and the administration group were higher than those in the normal group(all P<0.05).Compared with the administration group,the levels of proteus,firmicum,bacteroides,actinomycetes,CA19-9,AFP,ALT,TLR4/NF-κB signaling pathway levels in the normal group were lower than those in the model group,and proteus,firmicum,bacteroides,actinomycetes,CA19-9,AFP,ALT,TLR4/NF-κB signaling pathway in the model group were lower than those in the model group.The average level of road water was higher than that of the normal group(all P<0.05).Conclusion:Fuzheng Quxie decoction may repair liver cancer through TLR4/NF-κB signal pathway,improve the microecological environment of intestinal flora in rats,and play a protective role of liver function.

参考文献/References:

[1] 胡 峻,高月求,孙学华,等.肝积颗粒联合肝动脉化疗栓塞术治疗中晚期肝癌近期疗效研究[J].陕西中医,2020,41(9):1237-1240.
[2] 曹绍华,王庆庆,张金冉.索拉非尼联合卡培他滨治疗肝细胞肝癌疗效及对患者血清miR-212和miR-132水平的影响[J].陕西医学杂志,2020,49(5):615-618.
[3] 李 浩,江谋应,许朱定.手术与介入在原发多灶性肝癌治疗中疗效的比较分析[J].中国中西医结合外科杂志,2020,26(3):524-530.
[4] 林栋毅,彭 波,郑景辉,等.基于肝癌临床特征构建的logistic回归模型对原发性肝癌中医证型的判断效能[J].临床肝胆病杂志,2020,36(6):1293-1298.
[5] 贾 鹂,杨志宏,马俊利.贞芪扶正颗粒通过miR-200c/ZEB通路抑制肝癌模型大鼠血管形成[J].中国比较医学杂志,2020,30(6):31-38.
[6] 朱亚南,张 硕,张 敏,等.大鼠胆汁中黄芩苷代谢物的分离、纯化及其对肝癌细胞HepG2增殖的影响研究[J].中国药房,2020,31(7):800-805.
[7] Lee MH,DeBerardinis RJ,Wen X,et al.Active pyruvate dehydrogenase and impaired gluconeogenesis in orthotopichepatomas of rats[J].Metabolism,2019,101(1):153993.
[8] 文 峰,姜鹤群.肝癌大鼠癌细胞microRNA-21含量与TLR2、TLR4、NF-κB 水平的相关性研究[J].湖南师范大学学报:医学版,2019,16(4):10-13.
[9] 韦 武,郭红星.大鼠肝癌模型建立的研究进展[J].中国实验动物学报,2019,27(1):119-123.
[10] 梁智超,姬凤彩,孙建新,等.两种大鼠肝癌模型制作方法的对比研究[J].新疆医科大学学报,2019,42(1):130-133.
[11] 冯高飞,陈 若,陈伟钊.“扶正祛邪”治则在肝癌治疗中的应用[J].辽宁中医杂志,2015,42(5):953-954.
[12] Lupo MG,Biancorosso N,Brilli E,et al.Cholesterol-lowering action of a novel nutraceutical combination in uremicrats:insights into the molecular mechanism in a hepatoma cell line[J].Nutrients,2020,12(2):436.
[13] Kowalik MA,Puliga E,Cabras L,et al.Thyroid hormone inhibits hepatocellular carcinoma progression via induction ofdifferentiation and metabolic reprogramming[J].J Hepatol,2020,72(6):1159-1169.
[14] Mo'men YS,Hussein RM,Kandeil MA.A novel chemoprotective effect oftiopronin against diethylnitrosamine-induced hepatocellular carcinoma in rats:Role of ASK1/P38 MAPK-P53 signalling cascade[J].Clin Exp Pharmacol Physiol,2020,47(2):322-332.
[15] Avritscher R,Jo N,Polak U,et al.Hepatic arterial bland embolization increases Th17 cell infiltration in a syngeneic rat model of hepatocellular carcinoma[J].CardiovascIntervent Radiol,2020,43(2):311-321.
[16] 吴 聪,张荣臻,王挺帅,等.肠道微生态与肝细胞癌关系的研究进展[J].广西医学,2020,42(6):745-747.
[17] 裴 颖,黄 峰,殷宏振,等.去甲斑蝥酸钠对肝癌异位移植瘤小鼠肠道微生态的影响初探[J].实用肝脏病杂志,2019,22(4):486-489.
[18] 许发功,喇翠玲,郭新建.抑制AFP表达对肝癌小鼠模型肝脏损伤修复的作用机制[J].临床和实验医学杂志,2020,19(11):1165-1168.
[19] 王克净,唐乾利,韦杨年,等.腹腔镜肝癌切除术治疗老年肝细胞癌患者远期疗效及对CA19-9、GP73、SOD水平的影响[J].中国老年学杂志,2019,39(18):4429-4433.
[20] 文 峰,姜鹤群.肝癌大鼠癌细胞microRNA-21含量与TLR2、TLR4、NF-κB 水平的相关性研究[J].湖南师范大学学报:医学版,2019,16(4):10-13.
[21] 王 雨,许达峰,周开伦,等.TLR4和肠道菌群在原发性肝癌进展中动态变化及与预后的关系[J].肝脏,2020,25(3):273-276.
[22] 郑志明,邹海鹏,卢敏娟,等.佛手柑内酯抑制p62/NF-κB信号轴增强顺铂对肝癌杀伤的影响[J].安徽医科大学学报,2018,53(3):390-395.
[23] 王亚琪,曾普华,郜文辉.益气化瘀解毒方联合索拉非尼对人肝癌索拉非尼耐药细胞移植瘤及缺氧诱导因子1α与血管拟态表达的影响[J].陕西中医,2020,41(2):143-146.
[24] 李 珣,张祥林.肝动脉化疗栓塞联合恩替卡韦对原发性肝癌治疗效果及对患者血清中恶性肿瘤特异性生长因子的影响[J].陕西医学杂志,2019,48(7):873-875.
[25] 杨永昌,贾志凌,张 颖,等.肿瘤标志物AFP-L3、AFP、AFU在原发性肝癌早期诊断中的应用评价[J].中国急救复苏与灾害医学杂志,2016,11(7):682-684.

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备注/Memo

备注/Memo:
基金项目:湖北省自然科学基金资助项目(2018CFB791)
更新日期/Last Update: 2021-01-28