[1]杨俊锋,郝艳方,王福科,等.防己黄芪汤对三阴性乳腺癌细胞MB-MDA-231抑制作用研究[J].陕西中医,2021,(2):152-155,171.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.004]
　YANG Junfeng,HAO Yanfang,WANG Fuke,et al.Effects of Fangji Huangqi decoction to triple negative breast cancer MB-MDA-231 in vitro[J].,2021,(2):152-155,171.[doi:DOI:10.3969/j.issn.1000-7369.2020.02.004]
点击复制

防己黄芪汤对三阴性乳腺癌细胞MB-MDA-231抑制作用研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年2期
页码:
152-155,171
栏目:
基础研究
出版日期:
2021-02-05

文章信息/Info

Title:
Effects of Fangji Huangqi decoction to triple negative breast cancer MB-MDA-231 in vitro
作者:
杨俊锋; 郝艳方; 王福科; 裴晓华
(北京中医药大学房山医院外科,北京 102400)
Author(s):
YANG Junfeng; HAO Yanfang; WANG Fuke; PEI Xiaohua
(Department of Surgery,Fangshan Hospital of Beijing University of Traditional Chinese Medicine,Beijing 102400,China)
关键词:
防己黄芪汤;  三阴性乳腺癌;  MB-MDA-231细胞;  凋亡;  侵袭
Keywords:
Fangji Huangqi decoction;  Triple negative breast cancer;  MB-MDA-231 cells;  Apoptosis;  Invasiveness
分类号:
R 737.9
DOI:
DOI:10.3969/j.issn.1000-7369.2020.02.004
文献标志码:
A
摘要:
目的:研究防己黄芪汤对人三阴性乳腺癌细胞MB-MDA-231增殖、凋亡以及侵袭力的影响。方法:MTT法检测防己黄芪汤对人三阴性乳腺癌细胞MB-MDA-231的抑制作用; 倒置显微镜下观察细胞形态变化。将实验组分成4、8、16 mg/ml的不同浓度组,AnnexinV-FITC /PI双染检测MB-MDA-231细胞凋亡; Transwell法测定细胞体外迁移能力。结果: 防己黄芪汤可显著抑制MB-MDA-231细胞生长,在4、8、16、32、64 mg/ml浓度范围内呈浓度依赖性。防己黄芪汤能明显改变细胞形态,倒置显微镜下仅可见很少的贴壁细胞,多散在排列,部分细胞体积改变,胞浆内出现空泡变性,细胞结构不完整,裂解成碎片。防己黄芪汤作用24 h能够提高MB-MDA-231细胞的总凋亡率、早期凋亡率和晚期凋亡率,其中提高早期凋亡率更为明显。抑制MB-MDA-231细胞体外迁移的能力,高浓度组效果更佳,迁移细胞数明显减少。结论:防己黄芪汤能够抑制人三阴性乳腺癌细胞MB-MDA-231的增殖、迁移,并且促进其凋亡。
Abstract:
Objective:To research the effects of Fangji Huangqi decoction to triple negative breast cancer MB-MDA-231 in vitro.Methods:MTT was used to detect the inhibitory effect of Fangji Huangqi decoction on human triple-negative breast cancer cell MB-MDA-231.Observing the cell morphological changes by inverted microscope.The test group were divided into 4,8,16 mg/ml,low,medium,and high concentration groups.Annexin V-FITC/PI double staining was used to detect the apoptosis of MB-MDA-231 cells.The Transwell was used to determine the cell migration ability in vitro.Results:Fangji Huangqi decoction can significantly inhibit the growth of MB-MDA-231 cells in a concentration-dependent range of 4,8,16,32 and 64 mg/ml.Fangji Huangqi decoction can significantly change the morphology of cells.Only a few adherent cells can be seen under an inverted microscope.They are mostly scattered and arranged,some of the cells have changed in volume,vacuolar degeneration appears in the cytoplasm,and the cell structure is incomplete and broken into fragments.Fangji Huangqi decoction can increase the total apoptosis rate,early apoptosis rate and late apoptosis rate of MB-MDA-231 cells after 24 hours,and the improvement of early apoptosis rate is more obvious.The high concentration group has a better effect on inhibiting the ability of MB-MDA-231 cells to migrate in vitro,and the number of migrated cells is significantly reduced.Conclusion:Fangji Huangqi decoction can significantly inhibit MB-MDA-231,cause MB-MDA-231 apoptosis and nhibit the invasiveness of MB-MDA-231 in vitro.

参考文献/References:

[1] 李 旭,安改丽,黄尚科,等.阿帕替尼对三阴性乳腺癌MDA-MB-231细胞抑制作用体外研究[J].陕西医学杂志,2016,45(11):1445-1447.
[2] Schmadeka R,Harmon BE,Singh M.Triple-negative breast carcinoma:current and emerging concepts[J].Am J Clin Pathol,2014,141(4):462-477.
[3] 祝景伟,佟经伟,许培权.Hippo信号通路中TAZ、YAP在三阴性乳腺癌中的表达及意义[J].陕西医学杂志,2020,49(8):940-943,968.
[4] Bianchini G,Balko JM,Mayer IA,et al.Triple-negative breast cancer:Challenges and opportunities of a heterogeneous disease[J].Nat Rev Clin Oncol,2016,13(11):674-690.
[5] Prat A,Adamo B,Cheang MC,et al.Molecular characterization of basal-like and non-basal-like triple-negative breast cancer[J].Oncologist,2013,18(2):123-133.
[6] Oakman C,Viale G,Leo AD.Management of triple negative breast cancer[J].Breast,2010,19(5):312-321.
[7] Park JH,Ahn JH,Kim SB.How shall we treat early triple-negative breast cancer(TNBC):From the current standard to upcoming immuno-molecular strategies[J].ESMO Open,2018,3(1):357.
[8] 付培亭.防己黄芪汤加减治疗乳腺癌术后患肢水肿的临床疗效观察[D].南京:南京中医药大学,2014.
[9] 邓晓霞,李清宋,陈 中,等.黄芪抗肿瘤作用机制的研究进展[J].中药新药与临床药理,2016,27(2):307-312.
[10] 赵苏瑛,滕凤猛,李鹏飞,等.黄芪皂苷干预乳腺癌细胞MCF-7的体外实验研究[J].中医药导报,2016,22(10):33-37.
[11] 叶媚娜,陈红风,周瑞娟,等.黄芪多糖对基底细胞样乳腺癌细胞增殖和Akt磷酸化的影响[J].中西医结合学报,2011,9(12):1339-1446.
[12] 邢志博,王凤梅,王翠平,等.粉防己有效成分的药理活性研究进展[J].中国实验方剂学杂志,2014,20(9):241-246.
[13] 梁 晨.粉防己碱对三阴乳腺癌细胞HCC1937作用及生物学特性的影响[D].北京:北京中医药大学,2013.
[14] 梁 晨.三氧化二砷联合粉防己碱对乳腺癌血管新生及肺转移的影响[D].北京:北京中医药大学,2015.
[15] 张雪青,邵邻相,吴文才,等.白术挥发油抑菌及抗肿瘤作用研究[J].浙江师范大学学报:自然科学版,2016,39(4):436-442.
[16] 周小丽.白术抑瘤及抗肿瘤转移的实验研究[J].中医临床研究,2015,7(15):92-93.
[17] 王泰渊.中医药治疗乳腺癌相关文献证治用药之规律研究[D].南京:南京中医药大学,2012.
[18] Yamaue H,Tanimura H,Noguchi K,et al.Chemosensitivity testing of fresh human gastric cancer with highly purified tumour cells using the MTT assay[J].Br J Cancer,1992,66(5):794-799.
[19] 黄 芊,张扶莉,李雪莲,等.阳和汤对人乳腺癌MDA-MB-231细胞增殖、凋亡及其Akt/mTOR信号通路的影响[J].陕西中医,2018,39(11):1497-1500.
[20] Tsai YT,Lai JN,Lo PC,et al.Prescription of chinese herbal products is associated with a decreased risk of invasive breast cancer[J].Medicine(Baltimore),2017,96(35):e7918.
[21] 李晶洁,邢铁艳.防己黄芪汤加减治疗乳腺癌术后患肢水肿的疗效观察[J].陕西中医,2016,37(7):806-807.

相似文献/References:

[1]王 晶,方柯南,孙 洁.防己黄芪汤治疗小儿肾病综合征疗效及对患儿肾功能、凝血系统的影响*[J].陕西中医,2019,(9):1183.
　WANG Jing,FANG Kenan,SUN Jie..Clinical efficacy of Fangji Huangqi decoction and effect on renal function and coagulation system in treating pediatric nephrotic syndrome[J].,2019,(2):1183.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(81774319)
更新日期/Last Update: 2021-01-28