[1]张照庆,王小飞,尹 晶,等.针刀疗法对肩周炎模型兔肩关节囊局部组织中基因蛋白表达的影响[J].陕西中医,2021,(8):991-994,998.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.002]
　ZHANG Zhaoqing,WANG Xiaofei,YIN Jing,et al.Effects of acupotomy on the expression of genes and proteins in a rabbit model of scapulohumeral periarthritis[J].,2021,(8):991-994,998.[doi:DOI:10.3969/j.issn.1000-7369.2020.08.002]
点击复制

针刀疗法对肩周炎模型兔肩关节囊局部组织中基因蛋白表达的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年8期
页码:
991-994,998
栏目:
基础研究
出版日期:
2021-08-05

文章信息/Info

Title:
Effects of acupotomy on the expression of genes and proteins in a rabbit model of scapulohumeral periarthritis
作者:
张照庆; 王小飞; 尹 晶; 武 欢; 金 玮
(武汉市第三医院,湖北 武汉 430001)
Author(s):
ZHANG Zhaoqing; WANG Xiaofei; YIN Jing; WU Huan; JIN Wei
(Wuhan Third Hospital,Wuhan 430001,China)
关键词:
肩周炎;  针刀;  白细胞介素6;  高迁移率族蛋白1;  酸敏感离子通道蛋白3
Keywords:
Scapulohumeral periarthritis;  Acupotomy;  IL-6;  HLGB1;  ASIC3
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7369.2020.08.002
文献标志码:
A
摘要:
目的:研究针刀疗法对肩周炎兔肩关节囊中白细胞介素6(IL-6)、高迁移率族蛋白1(HLGB1)、酸敏感离子通道蛋白3(ASIC3)蛋白和基因表达的影响。方法:健康雄性新西兰大白兔30只,按照随机数字表法分为空白组、模型组、针刀组,每组各10只。采用机械加冰敷制造肩周炎模型。密切关注造模前后各组肢体功能活动变化; 采用HE染色观察局部组织形态学变化; 采用Western blot法检测局部IL-6、HLGB1、ASIC3蛋白水平; 采用RT-PCR技术检测各组关节囊滑膜中IL-6、HLGB1、ASIC3的mRNA表达水平。结果:针刀松解法能改善局部病理状态,减少滑膜和肌间质纤维素渗出、粘连和机化,促进组织的修复; 与空白组比较,模型组兔肩关节滑膜IL-6、HLGB1、ASIC3基因和蛋白表达水平显著增加(均P<0.05); 与模型组相比,针刀组兔肩关节滑膜IL-6、HLGB1、ASIC3基因和蛋白表达水平显著降低(均P<0.05)。模型组IL-6、HLGB1、ASIC3蛋白含量显著增多,基因表达水平明显上调,与空白组相比有统计学差异(均P<0.05); 针刀组上述指标蛋白含量显著降低,且对上述基因表达水平具有明显抑制作用,与模型组、空白组相比有统计学差异(均P<0.05)。结论:针刀疗法可降低肩关节囊滑膜中IL-6、HLGB1、ASIC3蛋白表达水平,并能抑制其基因过度表达,这可能是针刀治疗肩周炎有效作用机制之一。
Abstract:
Objective:To study the effects of acupotomology on the expression of IL-6,HLGB1,ASIC3 and genes in periarthritis humeral rabbits.Methods:30 healthy male New Zealand white rabbits.Divided into blank,model,and acupotomy groups according to the random number table method,with 10 pieces in each group.Fabrication of a frozen shoulder model using mechanical plus ice application.Pay close attention to the changes in functional activity of each group of limbs before and after modeling,local histomorphological changes were observed using HE staining,detection of local IL-6,HLGB1 and ASIC3 protein levels by Western blot method.The mRNA expression levels of IL-6,HLGB1 and ASIC3 in synovium of joint capsule were detected by RT-PCR.Results:Acupotomy release improves localized pathology of the shoulder capsule,reduces synovial and interstitial fibrillar effusion,adhesions and mechanization and promotes tissue repair.Significantly increased IL-6,HLGB1,ASIC3 gene and protein expression levels in the rabbit shoulder synovium in the model group compared with the blank group(all P<0.05).The expression levels of IL-6,HLGB1 and ASIC3 genes and proteins were significantly reduced in the rabbit shoulder synovium in the acupotomy group compared with the model group(all P<0.05).The content of IL-6,HLGB1,and ASIC3 proteins in the model group was significantly increased and the gene expression level was significantly up-regulated,which was significantly different from the blank group(all P<0.05),significantly lower protein content of the above indicators in the acupotomy group,and has a significant inhibitory effect on the expression level of these genes,significantly different compared to the model and blank group(all P<0.05).Conclusion:Acupotomy therapy reduces IL-6,HLGB1,ASIC3 protein expression levels in the shoulder bursa synovium,this may be one of the mechanisms by which acupuncture works effectively in treating scapulohumeral periarthritis.

参考文献/References:

[1] 马福长,金 涛,薛卫国.基于数据挖掘的针灸治疗肩关节周围炎临床选穴规律研究[J].陕西中医,2019,40(9):1284-1287.
[2] 张晓燕,张凌娟,张礼璐,等.中药热熨、针灸联合点揉法治疗老年肩周炎效果及对患者肩关节功能、炎症状态、疼痛递质的影响[J].陕西中医,2020,41(10):1492-1495.
[3] Cher JZ,Akbar M,Kitson SM,et al.Alarmins in frozen shoulder:A molecular association between inflammation and pain[J].American Journal of Sports Medicine,2018,46(3):671-678.
[4] Mullett H,Byrne D,Colville J.Adhesive capsulitis:Human fibroblast response to shoulder joint aspirate from patients with stage Ⅱ disease[J].Journal of Shoulder and Elbow Surgery,2007,16(3):290-294.
[5] Cho CH,Lho YM,Ha E,et al.Up-regulation of acid-sensing ion channels in the capsule of the joint in frozen shoulder[J].The Bone & Joint Journal,2015,97(6):824-829.
[6] 张增富.针刀与局部封闭法治疗肩周炎患者的疼痛介质及炎性因子影响的研究[J].中医临床研究,2018,10(8):82-84.
[7] 高丙南.舒筋汤为主配合针刀疗法治疗瘀滞型肩周炎痛症疗效观察[J].陕西中医,2017,38(2):224-225.
[8] 张里援,陈湘宜.水针刀结合通痹酊剂加热敷治疗肩周炎的疗效及对血浆β-内啡肽和P物质含量的影响[J].中华中医药学刊,2018,36(8):1841-1843.
[9] 史晓萍,宗阿南,陶 钧,等.《关于善待实验动物的指导性意见》的研究[J].中国医科大学学报,2007,36(4):493.
[10] 熊昌源,毕学薇.兔肩周炎的模型复制及相关生物化学指标测定[J].中国骨伤,1996,9(4):11-13.
[11] 何 勇,熊建义,崔家鸣,等.肩周炎肩关节活动受限的分子生物学研究[J].国际骨科学杂志,2016,37(3):187-189.
[12] 胡 波,郭长青,韩森宁,等.电针对肩周炎兔血清和肌肉组织中5-HT和PGE2含量的影响[J].上海针灸杂志,2013,32(2):146-148.
[13] 卞胡伟,蒋 涛,许建安,等.许建安教授“从寒论治”肩周炎经验[J].时珍国医国药,2017,28(12):2999-3000.
[14] 姜文清,谷学珍.论肩周炎的中医治疗[J].时珍国医国药,2012,23(12):3183-3184.
[15] 陈有国,陈爱华,云永兴,等.复方合谷巨刺局部麻醉运动针法治疗粘连期肩周炎的临床研究[J].针灸临床杂志,2019,35(1):10-14.
[16] 袁 红,陈 榕,黄大鹏,等.平衡针对家兔肩周炎的抗炎镇痛作用研究[J].中国针灸,2011,31(12):1106-1110.
[17] 何 丽,李 敏,孔 羽,等.针刀松解术联合电针治疗肩周炎的随机对照研究[J].针灸临床杂志,2019,35(2):33-36.
[18] 王正强,董 博,姚 洁,等.基于弓弦力学理论指导下针刀治疗膝骨性关节炎作用机制研究[J].陕西医学杂志,2019,48(9):1247-1249.
[19] Zhou YQ,Liu Z,Liu ZH,et al.Interleukin-6:An emerging regulator of pathological pain[J].Journal of Neuroinflammation,2016,13(1):141.
[20] Bridges D,Thompson SW,Rice AS.Mechanisms of neuropathic pain[J].Br J Anaesth,2001,87(1):12-26.
[21] 贺地震,刘谦性.沙利度胺联合甲氨蝶呤治疗类风湿性关节炎疗效及对患者调节性T细胞、白细胞介素-6水平影响临床研究[J].陕西医学杂志,2019,48(1):111-113.
[22] 田 淼,晁 旭,何春玲,等.急性白血病患者血清PDGF、HMGB1及相关血管内皮生长因子表达水平研究[J].陕西医学杂志,2017,46(4):508-509.
[23] Reichling DB,Green PG,Levine JD.The fundamental unit of pain is the cell[J].Pain,2013(154):2-9.
[24] Thankam FG,Dilisio MF,Dietz NE,et al.TREM-1,HMGB1 and RAGE in the shoulder tendon:Dual mechanisms for inflammation based on the coincidence of glenohumeral arthritis[J].PLoS One,2016,11(10):165492.

相似文献/References:

[1]孙 飞.肩三针温针灸治疗肩周炎疗效及对患者肩关节活动功能的影响[J].陕西中医,2019,(9):1294.
[2]王会丽,刘瑞芳,马爱琴.针刺联合弧刃针疗法治疗肩周炎疗效及对患者肩关节功能、活动度、肌力的影响*[J].陕西中医,2019,(10):1457.
[3]孙 实,赵环宇,王 琦.针刀治疗腰椎间盘突出症继发臀肌筋膜炎临床研究*[J].陕西中医,2020,(7):963.[doi:DOI:10.3969/j.issn.10007369.2020.07.038]
[4]桑 涛,马惠昇,宋毛毛,等.马惠昇采用毫火针齐刺经筋法辨证治疗肩周炎思路[J].陕西中医,2021,(5):638.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.024]
　SANG Tao,MA Huisheng,SONG Maomao,et al.Ma Huisheng's approach to dialectical treatment of frozen shoulder[J].,2021,(8):638.[doi:DOI:10.3969/j.issn.1000-7369.2020.05.024]
[5]邢皓宇,贾 超.肩胛平衡扬刺法埋线治疗肩周炎疗效研究[J].陕西中医,2023,(7):949.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.029]
　XING Haoyu,JIA Chao.Effect study on Yangci needling and Maixian based on scapular balance in treatment of scapulohumeral periarthritis[J].,2023,(8):949.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.029]
[6]孙晓薇,阚伯红,边明彤,等.针刺“肩三针”治疗肩周炎研究进展[J].陕西中医,2024,(8):1151.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.032]
　SUN Xiaowei,KAN Bohong,BIAN Mingtong,et al.Research progress of “shoulder three-needle” acupuncture in treatment of scapulohumeral periarthritis[J].,2024,(8):1151.[doi:DOI:10.3969/j.issn.1000-7369.2024.08.032]

备注/Memo

备注/Memo:
基金项目:十二五国家科技支撑计划项目(2014BAI08B06); 湖北省自然科学基金资助项目(2019CFC883); 武汉市卫生和计划生育委员会科研项目重点研究项目工程(WZ18B02)
更新日期/Last Update: 2021-08-09