[1]安 娜,黄芳敏,王健辉,等.四种中药不同药性与药理作用关系研究[J].陕西中医,2021,(10):1349-1353.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.006]
　AN Na,HUANG Fangmin,WANG Jianhui,et al.The study on the relationship between the different medicinal properties and pharmacological mechanism of four traditional Chinese medicines[J].,2021,(10):1349-1353.[doi:DOI:10.3969/j.issn.1000-7369.2020.10.006]
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四种中药不同药性与药理作用关系研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年10期
页码:
1349-1353
栏目:
基础研究
出版日期:
2021-10-05

文章信息/Info

Title:
The study on the relationship between the different medicinal properties and pharmacological mechanism of four traditional Chinese medicines
作者:
安 娜1; 黄芳敏2; 王健辉1; 沈 伟 2
(1.西安市第一医院 西北大学附属第一医院 陕西省眼科研究所 陕西省眼科学重点实验室 陕西省眼科疾病临床医学研究中心,陕西 西安 710002; 2.陕西省新药审评中心,陕西 西安 710065)
Author(s):
AN Na; HUANG Fangmin; WANG Jianhui; SHEN Wei
(Xi'an First Hospital,Xi'an 710002,China)
关键词:
药性;  肝郁脾虚;  信号通路;  药理学;  生物学效应
Keywords:
Medicinal property;  Liver depression and spleen deficiency;  Signaling pathway;  Pharmacology;  Biological effect
分类号:
R 28
DOI:
DOI:10.3969/j.issn.1000-7369.2020.10.006
文献标志码:
A
摘要:
目的:探讨丹参、柴胡、五味子、灵芝四种中药不同药性与药理学作用机制的关系。方法:采用20%四氯化碳(CCl4)香油溶液多次注射,饮用10%乙醇溶液并以高脂低蛋白饲料饲养小鼠制备肝郁脾虚模型,分别给予不同药性药物治疗10 d,酶法测定各实验组小鼠血清和肝匀浆中谷丙转氨酶(ALT)、乳酸脱氢酶(ALP)、胆碱酯酶(CHE)、谷胱甘肽(GSH); 体外培养肝星状细胞系(HSC-T6)并采用TGF-β1诱导其活化,Western blotting检测各实验组α-SMA、Ras、CollagenⅠ、ERK、p-ERK、TβRⅠ、TβRⅡ、Smad2/3蛋白的表达,观察药性性能不同的中药对HSC-T6表达Ras/ERK、TGF-β1/Smad信号通路蛋白的影响。结果:药性苦、寒柴胡,药性苦、微寒丹参处理肝郁脾虚模型小鼠,其生物学效应指标与中药药性论大致相似。药性酸、甘、温五味子处理肝郁脾虚模型小鼠,其生物学效应指标与中药酸、甘、温药性不尽一致,药性甘、平灵芝与前三者药性不同,其生物学效应指标显著差异。不同药性的药物处理HSC-T6靶细胞内Ras/ERK与TGF-β1/Smad信号通路蛋白表达存在着调节差异性。结论:依据中药药性的描述,通过研究影响机体生理变化和靶细胞内信号蛋白表达的意义,探讨不同药性的药理学作用机制,辨证地为广义中药药性赋予了药理学科学内涵。
Abstract:
Objective:To explore the relationship between the different medicinal properties of four traditional Chinese medicines(TCM)and their pharmacological mechanism with Salvia miltiorrhiza,Bupleurum,Schisandrae and Ganoderma lucidum.Methods:The model of liver depression and spleen deficie was prepared by injecting carbon tetrachloride(CCl4)and drinking 10% ethanol solution and feeding the mice with high-fat and low-protein diet.The mice were treated with different drugs for 10 days,respectively.ALT,ALP and CHE in serum and liver homogenate of the mice in each experimental group were determined by enzymatic method.HSC-T6 cell line was cultured in vitro and activated induced by the TGF-β1,Western blotting was used to detect the expressions of α-SMA,Ras,Collagen Ⅰ,ERK,p-ERK,TβRⅠ,TβRⅡ,Smad2/3 in each experimental group.To observe the effect of different properties of Chinese medicines on the expressions of Ras/ERK and TGF-β1/Smad signaling pathway proteins in HSC-T6 cell line.Results:The model mouse of liver depression and spleen deficie was treated by bitter,cold bupleurum and bitter,slight cold salvia miltiorrhiza,the biological effect indexs were roughly similar to the properties of TCM.The model mouse was treated by Sour,sweet,benign schisandrae,the biological effect indexs not consistent with the properties of TCM.The biological effect indexes of sweet,soft ganoderma lucidum were significantly different.Hsc-t6 cells treated with different drugs showed regulatory differences in expression of Ras/ERK and TGF- β1/Smad signaling pathway proteins.Conclusion:According to the description of the medicinal properties of TCM by investigating the significance of influencing the physiological changes of the body and the expression of signal proteins in target cells.The pharmacology mechanism of different medicinal properties was investigated,dialectically,pharmacology is endowed with scientific connotations for the broad sense of medicinal property of TCM.

参考文献/References:

[1] 王春燕.中药四性的研究概况[J].甘肃中医,2007,20(10):13.
[2] 孙广仁.中医基础理论[M].北京:中国中医药出版社,2006:245-249.
[3] 高晓山.中药药性论[M].北京:人民卫生出版社,1992:6.
[4] 王 鹏,张永清,商庆新,等.对中药药性科学内涵的再认识[J].陕西中医,2012,33(11):1545-1547.
[5] 唐仕欢,杨红军,黄璐琦.论中药药性的慨念、形成及其意义[J].中医杂志,2010,51(4):293-296.
[6] 严永清,吴建新.药物的辛味与归经、作用及化学成分的关系[J].中国中药杂志,1987,13(1):53-56.
[7] 王胜春,田卫斌,胡永武,等.柴胡、丹参、五味子加减对小鼠慢性肝损伤的作用[J].解放军药学学报,2002,18(1):13-16.
[8] 郑旭锐,惠 毅,闫曙光,等.三甲散对大鼠肝星状细胞HSC-T6生长增殖影响的研究[J].陕西中医,2014,35(9):1263-1265,1271.
[9] 高宇勤,郝霁萍,刘美林,等.芍药苷预处理通过干预NF-κB信号通路对在体大鼠MIRI发挥保护作用[J].陕西医学杂志,2015,44(12):1581-1582.
[10] 魏冬梅,余 春,童安荣.论升降出入[J].陕西中医,2010,31(12):1636-1638.
[11] 广东中医学院编.中医方药学[M].广州:广东人民出版社,1973:10-15.
[12] 苗彦霞.升降浮沉药性理论发微[J].陕西中医,2007,28(5):597-598.
[13] 刘 群,杨晓农.中药四气五味的现代认识[J].西南民族大学学报:自然科学版,2006,32(5):981-985.
[14] 王 鹏,张永清,商庆新,等.对中药药性科学内涵的再认识[J].陕西中医,2012,33(11):1545-1547.
[15] 许福泉,管华诗,冯媛媛,等.基于化学成分的中药归经研究[J].亚太传统医药,2012,8(5):197-199.
[16] Bataller R,Brenner DA.Hepatic stellate cells as a target for the treatment of 1iver fibrosis[J].Sem in Liver Dis,2001,21(3):437-451.
[17] Wu J,Zern MA.Hepatic stellate cells:A taget for the treatment of 1iver fibrosis[J].J Gastroentero1, 2000,35(9):665-672.
[18] Bissell DM,Roulot D,George J.Transforming growth factor β and the liver[J].Hepatol,2001,34:859-867.
[19] Dooley,Delvoux B,Streckert M,et al.Transforming growth factor-signal transduction in hepatic stellate cells via Smad2/3phosphorylation,a pathway that is abrogate durign in vitro porgression to myofibroblasts,factor-β signal transduction during transdefferentiation of hepatic stellate cells[J].FEBS Lett,2001,502(1-2):4-10.
[20] 田卫斌.大鼠肝纤维化形成过程中TGF-β1/ERK信号通路与Ⅰ、Ⅲ、Ⅳ型胶原表达变化[J].胃肠病学和肝病学杂志,2010,19(5):414-419.

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备注/Memo

备注/Memo:
基金项目:陕西省中医药科研项目(2019-ZZ-ZY013)
更新日期/Last Update: 2021-10-09