[1]袁文婷,肖茂良,肖 锋.半枝莲提取物通过PI3K-Akt信号通路抑制胰腺癌模型大鼠肿瘤生长的机制研究[J].陕西中医,2021,(12):1676-1679.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
　YUAN Wenting,XIAO Maoliang,XIAO Feng.Mechanism of scutellaria barbata extract inhibiting tumor growth through PI3K-Akt signaling pathway in pancreatic cancer model rats[J].,2021,(12):1676-1679.[doi:DOI:10.3969/j.issn.1000-7369.2020.12.005]
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半枝莲提取物通过PI3K-Akt信号通路抑制胰腺癌模型大鼠肿瘤生长的机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2021年12期
页码:
1676-1679
栏目:
基础研究
出版日期:
2021-12-05

文章信息/Info

Title:
Mechanism of scutellaria barbata extract inhibiting tumor growth through PI3K-Akt signaling pathway in pancreatic cancer model rats
作者:
袁文婷; 肖茂良; 肖 锋
(湖南中医药高等专科学校附属第一医院 湖南省直中医医院,湖南 株洲412000)
Author(s):
YUAN Wenting; XIAO Maoliang; XIAO Feng
(The First Affiliated Hospital of Hunan Traditional Chinese Medicinal College Hunan Province Directly Affiliated Traditional Chinese Medicinal Hospital,Zhuzhou 412000,China)
关键词:
胰腺癌;  半枝莲提取物;  细胞凋亡;  免疫功能;  细胞分化;  细胞自噬
Keywords:
Pancreatic cancer;  Scutellaria barbata extract;  Apoptosis;  Immune function;  Cell differentiation;  Cell autophagy
分类号:
R 735.9
DOI:
DOI:10.3969/j.issn.1000-7369.2020.12.005
文献标志码:
A
摘要:
目的:基于PI3K-Akt信号通路探究半枝莲提取物对胰腺癌模型大鼠的干预效果。方法:选取40只SD健康雄性大鼠,10只为正常组,其余30只建立胰腺癌模型,分为模型组、低浓度半枝莲组、高浓度半枝莲组。检测各组大鼠肿瘤体积、重量、T淋巴细胞亚群水平、细胞凋亡及Wnt3α、β-catenin、PI3K、Akt、mTOR表达。结果:高浓度半枝莲组大鼠肿瘤体积、重量低于低浓度半枝莲组、模型组(均P<0.05)。高浓度半枝莲组大鼠CD8+水平低于低浓度半枝莲组、模型组,CD4+、CD3+水平高于低浓度半枝莲组、模型组(均P<0.05)。高浓度半枝莲组大鼠Wnt3α、β-catenin、PI3K、Akt、mTOR表达低于低浓度半枝莲组、模型组(均P<0.05)。结论:半枝莲提取物可抑制胰腺癌模型大鼠肿瘤组织,调控胰腺癌大鼠T淋巴细胞亚群水平,调控Wnt/β-catenin、PI3K-Akt信号通路相关蛋白表达,促进胰腺癌细胞自噬、凋亡。
Abstract:
Objective:The intervention effect of scutellaria barbata extract on pancreatic cancer model rats was explored based on PI3K-Akt signaling pathway.Methods:40 SD healthy male rats were selected,10 cases normal groups,30 cases established pancreatic cancer models,which were divided into model group,low concentration group,high concentration group.The levels of tumor volume,weight,T lymphocyte subsets,apoptosis,Wnt3α,β-catenin,PI3K,Akt,mTOR were compared between the four groups.Results:The tumor volume and weight of high concentration scutellaria barbata group were lower than those of the low concentration scutellaria barbata group and the model group(all P<0.05).The level of CD8+ in high concentration scutellaria barbata group was lower than that in the low concentration scutellaria barbata group and the model group,the level of CD4+ and CD3+ in the high concentration scutellaria barbata group were higher than those in the low concentration scutellaria barbata group and the model group(all P<0.05).The expression of Wnt3α,β-catenin,PI3K,Akt and mTOR in the high concentration scutellaria chinensis groups were lower than those in the low concentration scutellaria chinensis group and the model group(all P<0.05).Conclusion:Scutellaria barbata extract was able to inhibit tumor tissue of the pancreatic cancer model rats,and regulate the level of T lymphocyte subsets in pancreatic cancer rats,regulate Wnt/β-catenin pathway and PI3K-Akt signaling pathway protein expression,promote apoptosis of pancreatic cancer cells.

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备注/Memo

备注/Memo:
基金项目:湖南省卫生和计划生育委员会科研基金资助项目(A2016004)
更新日期/Last Update: 2021-12-09