[1]葛子靖,王莘智,徐豫湘,等.通痹颗粒通过TRAF3/PTPN22信号通路调控类风湿关节炎大鼠T细胞自噬实验研究[J].陕西中医,2022,(2):157-160.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.004]
　GE Zijing,WANG Xinzhi,XU Yuxiang,et al.Tongbi granule regulates T cell autophagy in rheumatoid arthritis rats through TRAF3/PTPN22 signaling pathway[J].,2022,(2):157-160.[doi:DOI:10.3969/j.issn.1000-7369.2022.02.004]
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通痹颗粒通过TRAF3/PTPN22信号通路调控类风湿关节炎大鼠T细胞自噬实验研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年2期
页码:
157-160
栏目:
基础研究
出版日期:
2022-02-05

文章信息/Info

Title:
Tongbi granule regulates T cell autophagy in rheumatoid arthritis rats through TRAF3/PTPN22 signaling pathway
作者:
葛子靖; 王莘智; 徐豫湘; 范伏元; 田 英; 刘 丹
(湖南中医药大学第一附属医院,湖南 长沙 410007)
Author(s):
GE Zijing; WANG Xinzhi; XU Yuxiang; FAN Fuyuan; TIAN Ying; LIU Dan
(The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)
关键词:
佐剂性关节炎;  类风湿性关节炎;  通痹颗粒;  动物研究;  Wistar大鼠;  T细胞
Keywords:
Adjuvant arthritis;  Rheumatoid arthritis;  Tongbi granules;  Animal research;  Wistar rats;  T cell
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7369.2022.02.004
文献标志码:
A
摘要:
目的:研究通痹颗粒通过TRAF3/PTPN22信号通路调控类风湿关节炎大鼠T细胞自噬。方法:将佐剂性关节炎(AA)模型Wistar大鼠随机分成RA模型组、雷公藤多苷组、通痹颗粒组、3-MA组、联合组。另设正常对照组。治疗组大鼠采用成人剂量的3倍给药,2次/d,连续16 d。正常对照组以等容积的蒸馏水代替。16 d后处死各组大鼠,按要求留取血样及组织作相关指标检测。检测指标包括:足跖肿胀厚度、关节炎指数、测定血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1(IL-1),转化生长因子-β(TGF-β)的含量,测定大鼠关节组织肿瘤坏死因子受体相关因子3(TRAF3)、非受体型蛋白酪氨酸磷酸酶22(PTPN22)蛋白和自噬关键调控蛋白复合物(Beclin1)蛋白的表达。结果:治疗后各治疗组与RA模型组比较,大鼠右足跖厚度,关节炎指数,IL-1、TNF-α、TGF-β血清水平,以及TRAF3、PTPN22和Beclin1蛋白表达均明显改善(P<0.05)。雷公藤多苷组,3-MA组,通痹颗粒组三组大鼠之间比较上述各项指标均无统计学差异(P>0.05),联合组上述各项指标与其他治疗组比较均明显改善(P<0.05)。结论:通痹颗粒治疗RA模型大鼠的机制可能包括:上调TRAF3基因,下调PTPN22和Beclin1的表达,从而抑制T细胞自噬,使得T细胞的稳态得到恢复,减少炎症细胞因子的释放,缓解大鼠关节肿胀和关节炎状态,进而起到治疗RA的作用。
Abstract:
Objective:Research on Tongbi granule regulates T cell autophagy in rheumatoid arthritis rats through TRAF3/PTPN22 signaling pathway.Methods:The adjuvant arthritis(AA)model wistar rats were randomly divided into RA model group,tripterygium glycosides group,Tongbi granule group,3-MA group,combination group.A blank control group was also set up.Rats in the treatment group were administered 3 times of which given to the adult,twice a day,for 16 consecutive days.The normal control group was replaced by an equal volume of distilled water.Rats in each group were sacrificed 16 days later,and blood samples and tissues were collected as required for related index testing.The detection indicators include foot plantar swelling thickness,arthritis index,determination of serum tumor necrosis factor α(TNF-α),interleukin 1(IL-1),transforming growth factor-β(TGF-β)content,and determination of the content of tumor necrosis TNF-α,IL-1,and TGF-β.Mouse joint tissue tumor necrosis factor receptor-related factor 3(TRAF3),non-receptor protein tyrosine phosphatase 22(PTPN22)protein and autophagy key regulatory protein complex(Beclin1)protein expression were measured.Results:After treatment,each treatment group was compared with the model group,the thickness of the right foot plantar,arthritis index,serum levels of IL-1,TNF-α,TGF-β,and the protein expression of TRAF3,PTPN22 and Beclin1 were significantly improved(P<0.05).There was no significant difference in the above indicators between the tripterygium polyglycosides group,3-MA group and Tongbi granule group(P>0.05).Compared with other treatment groups,the above indicators in the combination group were significantly improved(P<0.05).Conclusion:The mechanism of Tongbi granules in the treatment of RA model rats may include:up-regulating the TRAF3 gene,down-regulating the expression of PTPN22 and Beclin1,thereby inhibiting T cell autophagy,thereby restoring T cell homeostasis and reducing the release of inflammatory cytokines,relieving rat joint swelling and arthritis state,and then playing a role in the treatment of RA.

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备注/Memo

备注/Memo:
基金项目:湖南省技术创新引导计划项目(2017SK50306); 湖南省自然科学基金资助项目(2020JJ4479); 湖南中医药大学校级课题(2018XJJJ37)
更新日期/Last Update: 2022-02-09