[1]赵海燕,张鸿婷,李佳卓,等.温化方通过NF-κB信号通路调控大鼠膝骨关节炎实验研究[J].陕西中医,2022,(6):696-699.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.004]
　ZHAO Haiyan,ZHANG Hongting,LI Jiazhuo,et al.Experimental study on effect of Wenhua prescription on knee osteoarthritis in rats through NF-κB signaling pathway[J].,2022,(6):696-699.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.004]
点击复制

温化方通过NF-κB信号通路调控大鼠膝骨关节炎实验研究
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年6期
页码:
696-699
栏目:
基础研究
出版日期:
2022-06-05

文章信息/Info

Title:
Experimental study on effect of Wenhua prescription on knee osteoarthritis in rats through NF-κB signaling pathway
作者:
赵海燕; 张鸿婷; 李佳卓; 魏明慧; 姜芊竹
(黑龙江中医药大学附属第二医院风湿科,黑龙江 哈尔滨 150001)
Author(s):
ZHAO Haiyan; ZHANG Hongting; LI Jiazhuo; WEI Minghui; JIANG Qianzhu
(Department of Rheumatology,Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China)
关键词:
膝骨关节炎;  温化方;  NF-κB信号通路;  Ⅱ型胶原蛋白酶;  大鼠
Keywords:
Knee osteoarthritis;  Wenhua prescription;  NF-κB signaling pathway;  Type Ⅱ collagenase;  Rat
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7369.2022.06.004
文献标志码:
A
摘要:
目的:探究温化方调控转录因子κB(NF-κB)信号通路对大鼠膝骨关节炎(KOA)的影响。方法:将SD大鼠随机分为对照组、模型组、温化方组、双氯芬酸钠组、抑制剂组,各10只。除对照组外,其余各组大鼠采用弗氏不完全佐剂乳化的牛Ⅱ型胶原蛋白诱导KOA大鼠模型。造模成功后开始给药,模型组给予0.9%氯化钠灌胃,观察组给予温化方灌胃,双氯芬酸钠组给予双氯芬酸钠灌胃,抑制剂组给予NF-κB抑制剂(吡咯烷二硫代氨基甲酸盐)灌胃,对照组大鼠自由饮水和进食。比较各组HE染色情况,大鼠体重、关节炎指数、关节肿胀度变化情况,酶联免疫吸附试验检测血清IL-1β、IL-6、TNF-α水平,荧光定量PCR法和Western blot检测大鼠关节滑膜组织中NF-κB信号通路相关基因和蛋白表达水平。结果:与模型组比较,对照组、温化方组、双氯芬酸钠组及抑制剂组的大鼠软骨损伤、纤维增生较低。与模型组比较,对照组、温化方组、双氯芬酸钠组、抑制剂组的大鼠体重较高,关节炎指数、关节肿胀度较低(均P<0.05)。与模型组比较,对照组、温化方组、双氯芬酸钠组、抑制剂组IL-1β、IL-6 和TNF-α水平较低(P<0.05)。与模型组比较,对照组、温化方组、双氯芬酸钠组、抑制剂组的NF-κB基因、蛋白表达水平均较低(P<0.05)。结论:温化方通过抑制NF-κB信号通路缓解大鼠KOA病情。
Abstract:
Objective:To explore effect of Wenhua prescription regulating transcription factor κB(NF-κB)signaling pathway on knee osteoarthritis(KOA)in rats.Methods:SD rats were randomly divided into blank control group,model group,Wenhua prescription group,diclofenac sodium group and inhibitor group,10 rats in each group.Except for the blank control group,the other groups of rats were induced by bovine type Ⅱ collagen emulsified with Freund's incomplete adjuvant to induce KOA rat model.After successful modeling,the drug was administered.The model group was given normal saline by gavage,the observation group given Wenhua prescription,the diclofenac sodium group given diclofenac sodium,and the inhibitor group given NF-κB inhibitor(pyrrolidine dithiocarbamate)by gavage,and the rats in the blank control group drank water and ate freely.The HE staining,changes of body weight,arthritis index and joint swelling in each group were compared.The serum IL-1β,IL-6 and TNF-α were detected by enzyme-linked immunosorbent assay.Fluorescence quantitative PCR and Western blot were used to detect the expression levels of NF-κB signaling pathway-related genes and proteins in the synovial tissue of rats.Results:Compared with the model group,the cartilage damage and fibrosis in the control group,Wenhua prescription group,diclofenac sodium group and inhibitor group were lower.The rats had higher body weight,lower arthritis index and lower joint swelling(all P<0.05).Compared with the model group,the control group,Wenhua prescription group,diclofenac sodium group and inhibitor group had lower lever of IL-1β,IL-6 and TNF-α(all P<0.05).Compared with the model group,the control group,Wenhua prescription group,diclofenac sodium group and inhibitor group had lower expression levels of NF-κB gene and protein(all P<0.05).Conclusion:Wenhua prescription alleviates KOA in rats by inhibiting NF-κB signaling pathway.

参考文献/References:

[1] Pavone V,Vescio A,Turchetta M,et al.Injection-based management of osteoarthritis of the knee:A systematic review of guidelines[J].Front Pharmacol,2021,12(2):661-668.
[2] 赵继宏,方 昕.补肾活血方辅助温针灸治疗膝骨性关节炎临床研究[J].陕西中医,2020,41(8):1101-1103.
[3] 徐志为,韩 杰,余绍涌,等.膝骨性关节炎中医药治疗研究进展[J].辽宁中医药大学学报,2021,22(12):205-208.
[4] 徐 旭,张丽丽,马红梅,等.温针灸联合加味阳和汤治疗老年膝关节骨性关节炎临床疗效及对炎症因子水平的影响[J].湖北中医药大学学报,2021,23(5):89-92.
[5] 张 良,郭丹丹,张鸿婷.温化法在膝骨关节炎关节积液中的运用[J].四川中医,2020,25(12):186-188.
[6] Eriksen EF,Lech O,Nakama GY,et al.Disease-modifying adjunctive therapy in osteoarthritisthe biological effects of a multi-mineral complexlitholexaljoint-a review[J].Clin Pract,2021,11(4):901-913.
[7] Ostojic M,Zevrnja A,Vukojevic K,et al.Immunofluorescence analysis of nf-kb and inos expression in different cell populations during early and advanced knee osteoarthritis[J].Int Journal Mol Sci,2021,22(12):578-580.
[8] 陈祖祥,葛彦志,周 莉,等.基于Wnt/β-catenin信号通路探讨去毒附子汤对骨关节炎大鼠的软骨保护作用[J].浙江中医药大学学报,2021,45(6):571-581.
[9] Henrotin Y,Antoine C,Zwerts E,et al.Oral supplementation with fish cartilage hydrolysate accelerates joint function recovery in rat model of traumatic knee osteoarthritis[J].Food Sci Nutr,2021,9(6):2877-2885.
[10] Yao N,Chen GC,Lu YY,et al.Bushen Qiangjin capsule inhibits the Wnt/α-catenin pathway to ameliorate papain-induced knee osteoarthritis in rat[J].Journal Tradit Chin Med,2021,41(6):935-942.
[11] 杨允尚.从瘀血论治膝关节骨性关节炎经验举隅[J].中国民族民间医药,2020,29(13):71-73.
[12] 邓超平,梁泳聪.健脾祛湿法在膝关节骨性关节炎治疗中的效果观察[J].北方药学,2020,17(6):68-69.
[13] 黄 建,杨丽薇,邓国忠.高频电针结合臭氧对膝骨关节炎患者血清IL-1及TNF-α表达的影响[J].陕西中医,2020,41(7):966-968.
[14] 刘芬之,郭珈宜,李 峰,等.中药熏洗联合西药治疗骨关节炎疗效及对患者IL-6、TNF-α的影响[J].陕西中医,2020,41(4):474-476.
[15] 赵 宇,李 洺,赵 江,等.G蛋白信号转导调节因子通过抑制MAPK和NF-κB通路缓解脂多糖诱导的人膝骨关节软骨细胞炎症反应实验研究[J].陕西医学杂志,2020,49(11):1387-1391.
[16] Barnabei L,Laplantine E,Mbongo W,et al.NF-κB:At the borders of autoimmunity and inflammation[J].Front Immunol,2021,12(20):7164-7169.
[17] Perez MF,Sarkies P.Malignancy and NF-κB signalling strengthen coordination between expression of mitochondrial and nuclear-encoded oxidative phosphorylation genes[J].Genome Biol,2021,22(1):328.
[18] Zinatizadeh MR,Schock B,Chalbatani GM,et al.The nuclear factor kappa bsignaling in cancer development and immune diseases[J].Genes Dis,2021,8(3):287-297.
[19] Thoms HC,Stark LA.The NF-κB nucleolar stress response pathway[J].Biomedicines,2021,9(9):123-129.
[20] Mimpen JY,Baldwin MJ,Cribbs AP,et al.Interleukin-17A causes osteoarthritis-like transcriptional changes in human osteoarthritis-derived chondrocytes and synovial fibroblasts in vitro[J].Front Immunol,2021,12(1):676-683.

相似文献/References:

[1]关春辉,周占锋,李 沛.益肾荣筋汤辅助胫骨高位截骨治疗膝骨关节炎疗效 及对患者关节液内细胞因子表达水平的影响*[J].陕西中医,2019,(5):616.
　GUAN Chunhui,ZHOU Zhanfeng,LI Pei..Effect of Yishen Rongjin decoction combined with high tibial osteotomy in the treatment of　knee osteoarthritis and the expression level of cytokines in articular fluid〖WT4”BZ〗[J].,2019,(6):616.
[2]李 君.壮骨关节胶囊治疗膝骨关节炎疗效及安全性Meta分析[J].陕西中医,2022,(3):394.[doi:DOI:10.3969/j.issn.1000-7369.2022.03.030]
[3]张 续,郑 洁,赵莉平,等.独活寄生汤对膝骨关节炎大鼠IL-17/NF-κB通路的影响[J].陕西中医,2022,(6):691.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.003]
　ZHANG Xu,ZHENG Jie,ZHAO Liping,et al.Effect of Duhuo Jisheng decoction on IL-17/NF-κB signaling pathway in rats with knee osteoarthritis[J].,2022,(6):691.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.003]
[4]冯文轩,肖春生.邓晋丰“通荣为要”治疗膝骨关节炎经验[J].陕西中医,2022,(6):778.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.025]
[5]张 帆,周胜利,周奕璇.膝骨关节炎中医外治研究进展[J].陕西中医,2022,(6):814.[doi:DOI:10.3969/j.issn.1000-7369.2022.06.034]
[6]邓 昶,胡胜利,谢 维,等.中医体质与膝骨关节炎相关性研究系统评价与Meta分析[J].陕西中医,2022,(8):1135.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.035]
　DENG Chang,HU Shengli,XIE Wei,et al.A systematic review and Meta-analysis of correlation between constitution of traditional Chinese medicine and knee osteoarthritis[J].,2022,(6):1135.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.035]
[7]姬 旭,武 琛,袁 芳,等.程海英教授运用贺氏三通法治疗膝骨关节炎经验[J].陕西中医,2022,(10):1452.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.032]
　JI Xu,WU Chen,YUAN Fang,et al.Professor CHENG Haiying's experience in treating knee osteoarthritis with HE's three-way method[J].,2022,(6):1452.[doi:DOI:10.3969/j.issn.1000-7369.2022.10.032]
[8]杨 娟,顾 伟,朱文俊,等.参蝎止痛胶囊治疗膝骨关节炎全膝关节置换术后疗效研究[J].陕西中医,2022,(12):1706.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.012]
　YANG Juan,GU Wei,ZHU Wenjun,et al.Effect study on Shenxie Zhitong capsule of knee osteoarthritis after total knee arthroplasty[J].,2022,(6):1706.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.012]
[9]赵文勇,雷雨欣,孙智平,等.李彦民治疗膝骨关节炎经验撷萃[J].陕西中医,2022,(12):1784.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.033]
　ZHAO Wenyong,LEI Yuxin,SUN Zhiping,et al.Li Yanmin's experience in treating knee osteoarthritis[J].,2022,(6):1784.[doi:DOI:10.3969/j.issn.1000-7369.2022.12.033]
[10]刘思冶,闫 奇,汤吉祥,等.膝痹通方治疗早期膝骨关节炎肝肾亏虚证临床研究[J].陕西中医,2023,(3):307.[doi:DOI:10.3969/j.issn.1000-7369.2023.03.008]
　LIU Siye,YAN Qi,TANG Jixiang,et al.Clinical study on Xibitong prescription in treating knee osteoarthritis of early Ganshen Kuixu type[J].,2023,(6):307.[doi:DOI:10.3969/j.issn.1000-7369.2023.03.008]

备注/Memo

备注/Memo:
基金项目:黑龙江省中医药学会青年中医药科技创新计划项目(ZHY19-037)
更新日期/Last Update: 2022-06-09