[1]高 辉,刘 恭,韩仙菊.黄芩素上调miR-625-5p表达干预支气管哮喘小鼠的机制研究[J].陕西中医,2022,(7):838-841.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.005]
　GAO Hui,LIU Gong,HAN Xianju.The mechanism of baicalein up-regulating miR-625-5p expression in bronchial asthma mice[J].,2022,(7):838-841.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.005]
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黄芩素上调miR-625-5p表达干预支气管哮喘小鼠的机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年7期
页码:
838-841
栏目:
基础研究
出版日期:
2022-07-05

文章信息/Info

Title:
The mechanism of baicalein up-regulating miR-625-5p expression in bronchial asthma mice
作者:
高 辉; 刘 恭; 韩仙菊
(石家庄市妇幼保健院药剂科,河北 石家庄 050011)
Author(s):
GAO Hui; LIU Gong; HAN Xianju
(Department of Pharmacy,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050011,China)
关键词:
支气管哮喘;  黄芩素;  miR-625-5p;  细胞增殖;  细胞迁移;  血小板衍生生长因子;  气道平滑肌细胞;  小鼠
Keywords:
Bronchial asthma;  Baicalein;  miR-625-5p;  Cell proliferation;  Cell migration;  Platelet-derived growth factor;  Airway smooth muscle cells;  Mice
分类号:
R 562.25
DOI:
DOI:10.3969/j.issn.1000-7369.2022.07.005
文献标志码:
A
摘要:
目的:探讨黄芩素调控miR-625-5p表达对支气管哮喘小鼠气道平滑肌细胞(ASMCs)增殖、迁移及炎性因子的影响。方法:培养小鼠原代ASMCs细胞,作为对照组,重组小鼠血小板衍生生长因子(PDGF)诱导ASMCs增殖,作为PDGF组; 分别采用10、20、40 μmol/L的黄芩素处理PDGF诱导ASMCs细胞。将miR-NC、miR-625-5p转染至PDGF诱导的ASMCs细胞,作为PDGF+miR-NC、PDGF+miR-625-5p组; 将anti-miR-NC、anti-miR-625-5p转染至PDGF诱导ASMCs细胞,以40 μmol/L的黄芩素处理,作为PDGF+anti-miR-NC+40 μmol/L组、PDGF+anti-miR-625-5p+40 μmol/L组。CCK-8法检测细胞增殖; Transwell实验检测细胞迁移; 酶联免疫吸附实验检测细胞IL-4、IL-6、IL-8水平; 实时荧光定量PCR检测细胞miR-625-5p表达水平。结果:与对照组比较,PDGF组细胞增殖率、迁移率以及IL-4、IL-6、IL-8水平显著升高(均P<0.05); 与PDGF组比较,黄芩素低、中、高剂量组细胞增殖率、迁移率以及IL-4、IL-6、IL-8水平显著降低(均P<0.05)。与对照组比较,PDGF组细胞miR-625-5p表达显著降低(P<0.05); 与PDGF组比较,黄芩素低、中、高剂量组细胞miR-625-5p表达显著升高(均P<0.05)。与PDGF+miR-NC组比较,PDGF+miR-625-5p组细胞增殖率、迁移率以及IL-4、IL-6、IL-8水平显著降低(均P<0.05)。与PDGF+anti-miR-NC+40 μmol/L组比较,PDGF+anti-miR-625-5p+40 μmol/L组细胞miR-625-5p表达水平显著降低,细胞增殖率、迁移率以及IL-4、IL-6、IL-8水平显著升高(均P<0.05)。结论:黄芩素通过上调miR-625-5p表达抑制支气管哮喘小鼠的细胞增殖率、迁移率以及炎性因子水平。
Abstract:
Objective:To investigate the effect of baicalein on the proliferation,migration and inflammatory factors of airway smooth muscle cells(ASMCs)in bronchial asthma mice by regulating the expression of miR-625-5p.Methods:Primary mouse ASMCs cells were cultured,as control group,recombinant mouse platelet-derived growth factor(PDGF)induced the proliferation of ASMCs,as PDGF group; 10,20,40 μmol/L of baicalein treated PDGF to induce ASMCs cells.miR-NC and miR-625-5p were transfected into PDGF-induced ASMCs cells as PDGF+miR-NC,PDGF+miR-625-5p group; anti-miR-NC and anti-miR-625-5p were transfected ASMCs cells were induced by PDGF,and then treated with 40 μmol/L of baicalein,as PDGF+anti-miR-NC+40 μmol/L group,PDGF+anti-miR-625-5p+40 μmol/L group.Cell proliferation was detected by CCK-8 method; Cell migration was detected by Transwell assay; IL-4,IL-6 and IL-8 levels were detected by enzyme-linked immunosorbent assay; Real-time quantitative PCR to detect the expression level of miR-625-5p in cells.Results:Compared with the control group,the cell proliferation rate,migration rate and the levels of IL-4,IL-6 and IL-8 in the PDGF group were significantly increased(all P<0.05).Compared with PDGF group,the proliferation rate,migration rate and IL-4,IL-6 and IL-8 levels of baicalin cells at were significantly decreased(all P<0.05).Compared with the control group,the expression of miR-625-5p in the PDGF group was significantly decreased(P<0.05); compared with the PDGF group,the expression of miR-625-5p in was significantly increased(all P<0.05).Compared with the PDGF+miR-NC group,the cell proliferation rate,migration rate and the levels of IL-4,IL-6 and IL-8 in the PDGF+miR-625-5p group were significantly decreased(all P<0.05).Compared with PDGF+anti-miR-NC+40 μmol/L group,the expression level of miR-625-5p in PDGF+anti-miR-625-5p+40 μmol/L group was significantly decreased,and the cell proliferation rate,migration rate and IL-4,IL-6,IL-8 levels were significantly increased(all P<0.05).Conclusion:Baicalein inhibited the proliferation rate,migration rate and inflammatory factors of bronchial asthma in mice by up-regulating the expression of miR-625-5p.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究重点课题计划项目(20210192)
更新日期/Last Update: 2022-07-07