[1]贾世艳,仲启明,司瑞花,等.基于转录组测序技术探讨复方蛇龙胶囊治疗膜性肾病大鼠潜在机制[J].陕西中医,2022,(7):842-846,901.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.006]
　JIA Shiyan,ZHONG Qiming,SI Ruihua,et al.Transcriptomic analysis exploring the molecular mechanisms of Shelong capsule in membranous nephropathy in rats[J].,2022,(7):842-846,901.[doi:DOI:10.3969/j.issn.1000-7369.2022.07.006]
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基于转录组测序技术探讨复方蛇龙胶囊治疗膜性肾病大鼠潜在机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年7期
页码:
842-846,901
栏目:
基础研究
出版日期:
2022-07-05

文章信息/Info

Title:
Transcriptomic analysis exploring the molecular mechanisms of Shelong capsule in membranous nephropathy in rats
作者:
贾世艳1; 2; 仲启明2; 司瑞花1; 2; 范晓阳2; 严文允1; 刘光珍2
(1.山西中医药大学,山西 晋中 030619; 2.山西省中医药研究院,山西 太原 030012)
Author(s):
JIA Shiyan; ZHONG Qiming; SI Ruihua; FAN Xiaoyang; YAN Wenyun; LIU Guangzhen
(Shanxi University of Chinese Medicine,Jinzhong 030619,China)
关键词:
膜性肾病;  复方蛇龙胶囊;  转录组测序;  大鼠;  差异基因;  关键基因;  PI3K/Akt信号通路
Keywords:
Membranous nephropathy;  Shelong capsule;  Transcriptome sequencing;  Rats;  Differentially expressed genes;  Hub genes;  PI3K/Akt signaling pathway
分类号:
R 692
DOI:
DOI:10.3969/j.issn.1000-7369.2022.07.006
文献标志码:
A
摘要:
目的:应用高通量转录组测序(RNA-seq)技术检测膜性肾病大鼠肾组织基因表达变化,分析复方蛇龙胶囊可能的作用机制。方法:采用改良Border法建立膜性肾病大鼠模型,设置模型组和复方蛇龙胶囊高、中、低剂量组(1.5、0.75、0.375 g/kg),每天灌胃1次,连续干预8周。采用RNA-seq技术检测复方蛇龙胶囊干预前后的差异表达基因,通过基因本体(GO)富集分析、京都基因与基因组百科全书(KEGG)通路富集分析、蛋白质相互作用(PPI)及关键基因分析,筛选关键基因以及关键通路。结果:与模型组相比,复方蛇龙胶囊低、中、高剂量组共筛选出差异表达基因685个,其中535个上调基因,150个下调基因。GO富集分析显示差异表达基因主要涉及细胞膜结构,参与物质跨膜运输、细胞粘附与迁移,调控跨膜转运蛋白活性,KEGG富集分析显示差异表达基因主要富集在PI3K/Akt和MAPK信号通路。蛋白质相互作用网络筛选出Egfr、Fn1、Rac3、Frk、Cdh1、Agt、Hspg2、Itga8、Akt3和Fgfr2共10个关键基因,GO功能富集分析显示关键基因主要富集在细胞膜表面及间质,参与跨胞外基质组织及粘附过程,调控激酶活性,KEGG富集分析结果显示关键基因主要富集在PI3K/Akt信号通路。结论:调控肾足细胞自噬与凋亡可能是复方蛇龙胶囊作用的重要机制,与PI3K/Akt信号通路相关基因表达有关。
Abstract:
Objective:To explore the possible mechanism of the Shelong capsule in the treatment of membranous nephropathy in rats by RNA-seq technology.Methods:The model of membranous nephropathy were established through the modified Border method.Model group and high-dose,medium-dose,and low-dose Shelong capsule group(1.5,0.75,0.375 g/kg)were set up,once a day,for consecutive 8 weeks.The differentially expressed genes after intervention of Shelong capsule were identified by RNA-seq technology.The gene oncology(GO),kyto encyclopedia of genes and genomes(KEGG)pathway enrichment and protein-protein interaction(PPI)network were carried out to gain insight into the actions of differentially expressed genes(DEGs).Results:Compared to the model group,685 common DEGs of three groups were determined by Venn analysis,including 535 up-regulated genes and 150 down-regulated genes.GO analysis showed that DEGs were mainly involved in membrane structure,transport,cell adhesion and migration,and they regulated the activity of transmembrane transporters.KEGG analysis indicated a few related pathway,including PI3K/Akt and MAPK signaling pathways were involved 10 genes(Egfr,Fn1,Rac3,Frk,Cdh1,Agt,Hspg2,Itga8,Akt3 and Fgfr2)were found as hub genes via cytohubba and functioned mainly by participating in cell membrane surface and PI3K/Akt signaling pathway.Conclusion:The mechanism underlying the therapeutic effect of Shelong capsule,including its effect on autophagy and apoptosis in podocyte injury,may be related to the PI3K/Akt signaling pathway.

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备注/Memo

备注/Memo:
基金项目:山西省重点研发计划重点项目(201603D3113023,201703D421024); 山西省科技攻关项目(2016ZD0307); 山西省晋药综合开发利用协同创新中心项目(2017-JYXT-32)
更新日期/Last Update: 2022-07-07