[1]王 芬,何华亮,刘铜华,等.中药复方糖耐康对KKAy糖尿病小鼠低度炎症信号通路的影响[J].陕西中医,2022,(8):992-996.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.002]
　WANG Fen,HE Hualiang,LIU Tonghua,et al.Effects of traditional Chinese medicine of Tangnaikang on low-degree inflammatory signaling pathway in KKAy diabetic mice[J].,2022,(8):992-996.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.002]
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中药复方糖耐康对KKAy糖尿病小鼠低度炎症信号通路的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年8期
页码:
992-996
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Effects of traditional Chinese medicine of Tangnaikang on low-degree inflammatory signaling pathway in KKAy diabetic mice
作者:
王 芬1; 何华亮2; 刘铜华3; 吴丽丽3
(1.北京中医药大学第三附属医院,北京 100029; 2.解放军武装警察部队北京市总队医院,北京 100027; 3.北京中医药大学,北京 100029)
Author(s):
WANG Fen; HE Hualiang; LIU Tonghua; WU Lili
(Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)
关键词:
2型糖尿病;  糖耐康;  低度炎症信号通路;  胰岛素抵抗;  小鼠;  肿瘤坏死因子-α
Keywords:
Diabetes mellitus type 2;  Tangnaikang;  Low-grade inflammatory signaling pathway;  Insulin resistance;  Mice;  Tumor necrosis factor-α
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7369.2022.08.002
文献标志码:
A
摘要:
目的:观察中药复方糖耐康对KKAy小鼠血糖、体重及低度炎症通路中关键因子的影响。方法:选取自发性2型糖尿病动物模型KKAy小鼠65只及 C57BL/6J 小鼠13只屏障系统饲养,给予高脂饲料喂养,连续给药8周后,评价静脉空腹血糖(禁食8 h以上)及体重变化,采用半定量酶联免疫吸附剂测定(ELISA)检测血浆中肿瘤坏死因子-α(TNF-α)及白介素-1β(IL-1β)、单核细胞趋化因子1(MCP-1)的水平。蛋白免疫印迹法检测肌肉组织核因子-κB(NF-κB)蛋白的表达水平。结果:中药糖耐康给药组中,尤其高剂量组,能够显著降低模型组血糖水平(P<0.05),具有一定控制体重增长趋势的作用; 可下调炎性因子TNF-α、IL-1β及MCP-1的水平(P<0.05),下调肌肉组织中NF-κB蛋白的表达水平(P<0.05)。结论:糖耐康可能通过抑制炎症信号通路中关键炎性因子的表达水平,抑制机体低度炎症反应,从而起到改善胰岛素抵抗及抗糖尿病的作用。
Abstract:
Objective:To observe the effect of TCM Tangnaikang on blood sugar,weight and low-degree inflammatory signaling pathway in KKAy mice.Methods:Choosed 65 KKAy mice and 13 C57BL/6J mice,a spontaneous type 2 diabetes animals were fed with high fat foods.After 8 weeks,blood sugar and weight changes were evaluated and TNF-α and IL-1β,MCP-1 in plasma were measured by ELISA semi-quantitative method.The expression level of the tissue NF-κB protein was detected by western blot method.Results:Especially in high dose group,Tangnaikang could significantly reduce blood glucose level(P<0.05),and has the role in controlling weight growth trend.Decrease the levels of inflammatory factors such as TNF-α,IL-1β and MCP-1(P<0.05).Decreased the expression of NF-κB protein in muscle tissue(P<0.05).Conclusion:Tangnaikang may correct the low-degree inflammatory signaling pathway of the body to improve insulin resistance and anti-diabetes.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金青年科学基金资助项目(82004338); 教育部优秀青年教师基金资助项目(2018-JYBZZ-JS169); 中医药防治内分泌代谢平台建设与创新中药和保健产品研发项目(2020-ZXFFJJ-005)
更新日期/Last Update: 2022-08-10