[1]夏秀丽,赵树山,李 云,等.黄芪多糖对小鼠食管癌前病变的影响及机制研究[J].陕西中医,2022,(8):1003-1008.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.004]
　XIA Xiuli,ZHAO Shushan,LI Yun,et al.Effect and mechanism of astragalus polysaccharides on precancerous lesions of esophagus in mice[J].,2022,(8):1003-1008.[doi:DOI:10.3969/j.issn.1000-7369.2022.08.004]
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黄芪多糖对小鼠食管癌前病变的影响及机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年8期
页码:
1003-1008
栏目:
基础研究
出版日期:
2022-08-05

文章信息/Info

Title:
Effect and mechanism of astragalus polysaccharides on precancerous lesions of esophagus in mice
作者:
夏秀丽; 赵树山; 李 云; 宋晓明; 徐 珊; 程丽敏; 李 森; 徐 超
(邯郸市中心医院,河北 邯郸 056001)
Author(s):
XIA Xiuli; ZHAO Shushan; LI Yun; SONG Xiaoming; XU Shan; CHENG Limin; LI Sen; XU Chao
(Handan Central Hospital,Handan 056001,China)
关键词:
食管癌前病变;  黄芪多糖;  信号传导和转录激活因子3;  缺氧诱导因子1α;  血管内皮生长因子
Keywords:
Esophageal precancerous lesions;  Astragalus polysaccharide;  STAT3;  Hypoxia-inducible factor 1α;  Vascular endothelial growth factor
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7369.2022.08.004
文献标志码:
A
摘要:
目的:探讨黄芪多糖(APS)对小鼠食管癌前病变的影响及其作用机制。方法: 将100只C57BL/6小鼠随机分为正常组26只、模型组26只、黄芪多糖组24只和全反式维甲酸组24只。除正常组外,其他组通过4-硝基喹啉-1-氧化物(4NQO)喂养15周诱导制备食管癌前病变小鼠模型。造模完成后,黄芪多糖组50 mg/kg灌胃给药,1次/d; 全反式维甲酸组7.5 mg/kg灌胃给药,每2 d 1次; 正常组和模型组灌胃给予0.9%氯化钠溶液,1次/d,共10周。HE染色法观察食管上皮组织病理学改变,透射电子显微镜观察食管上皮细胞超微结构改变; 流氏细胞仪检测血液中CD3+、CD4+、CD8+T细胞和NK细胞比例; RT-PCR法、Western blot法检测食管上皮组织信号传导和转录激活因子3(STAT3)、缺氧诱导因子1α(HIF-1α)、血管内皮生长因子(VEGF)mRNA和蛋白表达。结果: 与正常组比较,模型组小鼠食管上皮组织呈现癌前病变或癌变,癌变率66.67%; 食管上皮细胞可见胞核大小不等、核分裂相增多等超微结构改变; 血液中CD3+、CD4+T细胞和NK细胞比例降低,CD4+/CD8+比值降低,CD8+ T细胞比例升高(P<0.05); 食管上皮组织STAT3、HIF-1α、VEGF mRNA和蛋白表达量均升高(P<0.05)。与模型组比较,黄芪多糖组和全反式维甲酸组癌变率降低(P<0.05); 上皮细胞超微结构改变明显改善; CD3+、CD4+ T细胞和NK细胞比例均升高,CD4+/CD8+比值升高,CD8+ T细胞比例降低(P<0.05); STAT3、HIF-1α、VEGF mRNA和蛋白表达量均降低(P<0.05)。黄芪多糖组对各指标的调控作用均优于全反式维甲酸组(P<0.05)。结论: 黄芪多糖对小鼠食管癌前病变进展具有抑制作用,可能与提高细胞免疫功能及抑制STAT3、HIF-1α、VEGF转录表达有关。
Abstract:
Objective:To investigate the effect of astragalus polysaccharide(APS)on mouse esophageal precancerous lesions and its mechanism.Methods:A total of 100 C57BL/6 mice were randomly divided into normal group(n=26),model group(n=26),APS group(n=24)and all-trans retinoic acid(ATRA)group(n=24).Except for the normal group,the mice in other groups were induced by 4-Nitroquinoline 1-Oxide(4NQO)for 15 weeks to prepare mice models with esophageal precancerous lesions.After the modeling was completed,the mice in APS group were intragastrically administered with 50 mg/kg once a day; the mice in ATRA group were administered intragastrically with 7.5 mg/kg once every 2 days; the mice in normal group and model group were intragastrically administered with physiological saline once a day,10 weeks of treatment.The histopathological changes of esophageal epithelium was observe by HE staining.The ultrastructural changes of esophageal epithelial cells was observed by transmission electron microscope.The proportion of CD3+,CD4+,CD8+ T cells and natural killer(NK)cells in the blood were detected by flow cytometer.The mRNA and protein expression of signal transduction and activator of transcription factor 3(STAT3),hypoxia-inducible factor 1α(HIF-1α),vascular endothelial growth factor(VEGF)were detected by RT-PCR method and western blot method respectively.Results:Compared with the normal group,the esophageal epithelial tissue of the model group showed precancerous lesions or cancer,and the cancer rate was 66.67%.The ultrastructural changes such as the nucleus size was different,increased mitotic phase.The proportion of CD3+,CD4+ T cells and NK cells in the blood were decreased,the ratio of CD4+/CD8+ was decreases,and the proportion of CD8+ T cells was increased(P<0.05).The mRNA and protein expression of STAT3,HIF-1α,VEGF were increased(P<0.05).Compared with the model group,the canceration rate of esophageal epithelial tissue in APS group and ATRA group was decreased(P<0.05); the ultrastructural changes of epithelial cells were significantly improved; the proportion of CD3+,CD4+ T cells and NK cells were increased,the CD4+/CD8+ ratio was increased and the proportion of CD8+ T cells was decreased(P<0.05); the mRNA and protein expressions of STAT3,HIF-1α,VEGF were all decreased(P<0.05).The APS group had better regulatory effects on all indicators than that of ATRA group(P<0.05).Conclusion:APS has inhibitory effect on the progression of esophageal precancerous lesions in mice,which may be related to improving cellular immune function,inhibiting the transcription and expression of STAT3,HIF-1α,VEGF.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究课题(20200457)
更新日期/Last Update: 2022-08-10