[1]王祯芝,张美玲,熊 康,等.嗅三针对蛛网膜下腔出血并发认知障碍大鼠海马神经元及Caspase-3表达的影响[J].陕西中医,2022,(9):1159-1162.[doi:DOI:10.3969/j.issn.1000-7369.2022.09.002]
　WANG Zhenzhi,ZHANG Meiling,XIONG Kang,et al.Effect of Xiusan Zhen on hippocampal neurons and Caspase-3 expression in rats with subarachnoid hemorrhage concurrent cognitive impairment[J].,2022,(9):1159-1162.[doi:DOI:10.3969/j.issn.1000-7369.2022.09.002]
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嗅三针对蛛网膜下腔出血并发认知障碍大鼠海马神经元及Caspase-3表达的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年9期
页码:
1159-1162
栏目:
基础研究
出版日期:
2022-09-05

文章信息/Info

Title:
Effect of Xiusan Zhen on hippocampal neurons and Caspase-3 expression in rats with subarachnoid hemorrhage concurrent cognitive impairment
作者:
王祯芝1; 张美玲1; 熊 康1; 王 渊1; 王 强1; 周 锋2
(1.陕西中医药大学,陕西 咸阳 712046; 2.陕西中医药大学附属医院,陕西 咸阳 712021)
Author(s):
WANG Zhenzhi; ZHANG Meiling; XIONG Kang; WANG Yuan; WANG Qiang; ZHOU Feng
(Shaanxi University of Chinese Medicine,Xianyang 712046,China)
关键词:
蛛网膜下腔出血;  认知障碍;  嗅三针;  PI3K/AKT信号通路;  半胱氨酸蛋白-3
Keywords:
Subarachnoid hemorrhage;  Cognitive impairment;  Xiusan Zhen;  PI3K/AKT signalling pathway;  Caspase-3
分类号:
R 743.75
DOI:
DOI:10.3969/j.issn.1000-7369.2022.09.002
文献标志码:
A
摘要:
目的:研究嗅三针对蛛网膜下腔出血并发认知障碍模型大鼠的干预作用及对海马神经元Caspase-3、PI3K、AKT mRNA和蛋白表达的影响。方法:取清洁级雄性SD大鼠60只,随机分为空白组、模型组、嗅三针组、尼莫地平组以及假针刺组,每组12只。各组大鼠干预后,采用荧光定量PCR检测大鼠海马区Caspase-3、PI3K、AKT mRNA表达,Western blot技术检测Caspase-3、PI3K、AKT蛋白表达量。结果:与模型组比较,嗅三针组和尼莫地平组海马中PI3K、AKT mRNA表达量均有不同程度升高,且嗅三针组高于尼莫地平组,差异有统计学意义(均P<0.05),嗅三针组和尼莫地平组Caspase-3 mRNA表达均低,差异有统计学意义(均P<0.05)。与模型组比较,尼莫地平组及嗅三针组PI3K、AKT蛋白的表达均升高,Caspase-3蛋白表达降低,差异有统计学意义(均P<0.05)。结论:嗅三针对蛛网膜下腔出血并发认知障碍大鼠的神经保护作用机制可能是通过激活大鼠脑组织的PI3K/AKT信号通路,抑制Caspase-3蛋白表达及细胞凋亡,减轻神经组织炎性损伤。
Abstract:
Objective:To study the intervention effect of Xiusan Zhen on subarachnoid hemorrhage concurrent cognitive impairment model rats and the effects on mRNA and protein expressions of Caspase-3,PI3K and AKT in hippocampal neurons.Methods:60 clean grade male SD rats were randomly divided into blank group,model group,sniffing group,nimodipine group and Xiusan Zhen group,12 rats in each group.Each group after corresponding intervention,the mRNA expression levels of Caspase-3,PI3K and AKT in the hippocampus of rats were detected by fluorescence quantitative PCR,protein expression levels of Caspase-3,PI3K and AKT were detected by Western blot.Results:Compared with the model group,the mRNA expression of PI3K and AKT in the hippocampus of the Xiusan Zhen group and the nimodipine group were both increased to different degrees,and the Xiusan Zhen group was higher than the nimodipine group,difference statistically significant(all P<0.05).The mRNA expression of Caspase-3 in Xiusan Zhen group and the nimodipine group was significantly lower,and difference statistically significant(all P<0.05).Compared with the model group,the protein expression of PI3K and AKT were significantly higher in the nimodipine group and the Caspase-3 protein expression was significantly lower than those in the nimodipine group,difference statistically significant(all P<0.05).Conclusion:The main mechanism of neuroprotective effect of Xiusan Zhen on cognitive impairment in rats with subarachnoid hemorrhage may be through activation of PI3K/AKT signaling pathway and inhibition of Caspase-3 protein expression in rat brain tissue and apoptosis,reducing inflammatory damage in neural tissue.

参考文献/References:

[1] Etminan N,Macdonald RL.Management of aneurysmal subarachnoid hemorrhage[J].Handbook of Clinical Neurology,2017,140(1):195-228.
[2] Olsen MH,Lilja CA,Bache S,et al.Aneurysmal subarachnoid haemorrhage[J].Ugeskrift for Laeger,2019,181(31):1190-1199.
[3] Macdonald RL,Schweizer TA.Spontaneous subarachnoid hemorrhage[J].The Lancet,2017,389(10069):655-666.
[4] 梁兆俊,袁 雨,李佳仪,等.大鼠蛛网膜下腔出血早期脑损伤及炎症反应特点[J].中国医科大学学报,2020,49(1):62-66.
[5] 李 杰,王 渊,鲁 刚,等.嗅三针疗法对血管性痴呆大鼠学习记忆功能及Nrf2表达的影响[J].现代中医药,2020,40(2):29-32.
[6] 郑运松,徐 辉,赵 腾,等.嗅三针治疗血管性痴呆的临床研究[J].中医药导报,2020,26(4):57-60.
[7] Sang MH,Hoyee W,Gen K,et al.Molecular alterations in the hippocampus after experimental subarachnoid hemorrhage[J].Journal of Cerebral Blood Flow&Amp,2014,34(1):514-520.
[8] 罗 卫,孙 鹏,沈 冰.大鼠蛛网膜下腔出血后迟发性脑血管痉挛和rHSG表达相关性的实验研究[J].中风与神经疾病杂志,2009,26(6):660-663.
[9] 张议元,杜元灏.针灸治疗轻度认知障碍的处方规律初探[J].天津中医药,2020,37(1):65-70.
[10] 王振垚,张 虎,王新伟,等.针刺联合认知康复训练治疗卒中后认知障碍疗效及对细胞因子的影响[J].上海针灸杂志,2019,38(10):1098-1102.
[11] 王瑞瑞,毛忠南,王瑞仙.脑卒中后认知障碍中药治疗机制的研究进展[J].现代临床医学,2020,46(2):146-149.
[12] 王祯芝,张美玲,熊 康,等.针刺对脑卒中后认知障碍的作用机制研究进展[J].江苏中医药,2022,54(3):73-77.
[13] 刘智斌,牛文民,杨晓航,等.嗅三针对阿尔茨海默病患者认知功能影响的临床研究[J].陕西中医,2008,29(6):711-712.
[14] 杨晓航,牛文民,刘智斌,等.针药合用刺激嗅觉系统对血管性痴呆大鼠学习记忆功能及海马ChAT、AchE活性的影响[J].陕西中医,2009,30(10):1413-1414.
[15] 牛文民,刘智斌,杨晓航,等.嗅三针疗法对AD大鼠海马ChAT和SOD活性的影响[J].陕西中医学院学报,2013,36(6):93-95.
[16] Tannenholz L,Jimenez JC,Kheirbek MA.Local and regional heterogeneity underlying hippocampal modulation of cognition and mood[J].Frontiers in Behavioral Neuroscience,2014,54(8):147.
[17] 谢江涛,王云莉,苏永永,等.颅内动脉瘤性蛛网膜下腔出血患者术后早期认知功能障碍与铁蛋白表达的相关性研究[J].陕西医学杂志,2017,46(6):735-736.
[18] Yuksel S,Tosun YB,Cahill J,et al.Early brain injury following aneurysmal subarachnoid hemorrhage:Emphasis on cellular apoptosis[J].Turk Neurosurg,2012,22(5):529-533.
[19] Ayer R,Zhang J.Connecting the early brain injury of aneurysmal subarachnoid hemorrhage to clinical practice[J].Turk Neurosurg,2010,20(2):159-166.
[20] Duris K,Manaenko A,Suzuki H,et al.Alpha7 nicotinic acetylcholine receptor agonist PNU-282987 attenuates early brain injury in a perforation model of subarachnoid hemorrhage in rats[J].Stroke,2011,42(12):3530-3536.
[21] Khalili MA,Anvari M,Hekmati SH,et al.Therapeutic benefit of intravenous transplantation of mesenchymal stem cells after experimental subarachnoid hemorrhage in rats[J].Journal Stroke Cerebrovasc Dis,2012,21(6):445-451.
[22] Suzuki H,Hasegawa Y,Kanamaru K,et al.Mechanisms of osteopontin-induced stabilization of blood-brain barrier disruption after subarachnoid hemorrhage in rats[J].Stroke,2010,41(8):1783-1790.
[23] Guo S,Lo EH.Dysfunctional cell signaling in the neurovascular unit as a paradigm for central nervous system disease[J].Stroke,2009,40(3):4-7.
[24] Chen S,Fen H,Sherchan P,et al.Controversies and evolving new mechanisms in subarachnoid hemorrhage[J].Prog Neurobiol,2014,115(1):64-91.
[25] Mellergard P,Sjogren F,Hillman J.Release of VEGF and FGF in the extracellular space following severe subarachnoidal hemorrhage or traumatic head injury in humans[J].Br Journal Neurosurg,2010,24(3):261-267.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助面上项目(81873387); 国家中医药创新骨干人才项目[国中医药人教函(2019)128号]; 陕西省咸阳市中青年科技领军人才培育项目(重大科技创新专项2019K01-52); 陕西中医药大学培养计划项目(2017SZKY-018)
更新日期/Last Update: 2022-09-08