[1]周丽娜,辛 欢,杨 敏,等.丹参酚酸A对脑缺血大鼠神经功能及热休克蛋白基因表达的影响[J].陕西中医,2022,(11):1521-1526.[doi:DOI:10.3969/j.issn.1000-7369.2022.11.007]
　ZHOU Lina,XIN Huan,YANG Min,et al.Effects of salvianolic acid A on nerve function and heat shock protein gene expression in rats with cerebral ischemia[J].,2022,(11):1521-1526.[doi:DOI:10.3969/j.issn.1000-7369.2022.11.007]
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丹参酚酸A对脑缺血大鼠神经功能及热休克蛋白基因表达的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2022年11期
页码:
1521-1526
栏目:
基础研究
出版日期:
2022-11-05

文章信息/Info

Title:
Effects of salvianolic acid A on nerve function and heat shock protein gene expression in rats with cerebral ischemia
作者:
周丽娜; 辛 欢; 杨 敏; 方 芳; 张 艺
(武汉市第一医院 武汉市中西医结合医院,湖北 武汉 430030)
Author(s):
ZHOU Lina; XIN Huan; YANG Min; FANG Fang; ZHANG Yi
(Wuhan First Hospital,Wuhan Integrated Traditional Chinese and Western Medicine Hospital,Wuhan 430030,China)
关键词:
脑缺血;  丹参;  神经功能;  大鼠;  热休克蛋白;  基因表达
Keywords:
Cerebral ischemia;  Salvia miltiorrhiza;  Nerve function;  Rats;  Heat shock protein;  Gene expression
分类号:
R 255.2
DOI:
DOI:10.3969/j.issn.1000-7369.2022.11.007
文献标志码:
A
摘要:
目的:探讨丹参酚酸A对脑缺血大鼠神经功能及热休克蛋白基因表达的影响,为其脑保护机制研究提供参考。方法:选用80只健康成年雄性SD大鼠作为研究对象,通过线栓法对动脉缺血再灌注损伤模型进行制备,并采用随机数字表法将其分为假手术组、模型组、低剂量组和高剂量组,每组各20只。比较各组大鼠实验后24 h脑梗死体积和实验72 h后胶质源性神经营养因子(GDNF)、脑源性神经营养因子(BDNF)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化物歧化酶(SOD)水平,分析实验24、48、72 h后神经行为学评分变化。在再灌注24、48、72 h后取大鼠脑组织,采用免疫组织化学染色和原位杂交法对大鼠脑组织HSP70蛋白和HSP70 mRNA水平进行测定。结果:低剂量组、高剂量组大鼠脑梗死面积均低于模型组,且高剂量组脑梗死体积明显更低。实验24、48、72 h后,低剂量组、高剂量组大鼠神经行为学评分均低于模型组,且高剂量组神经行为学评分明显更低。模型组、低剂量组、高剂量组大鼠脑组织BDNF、GDNF、MDA水平均明显高于假手术组,SOD、GSH-Px水平明显低于假手术组; 且模型组、低剂量组、高剂量组脑组织大鼠BDNF、GDNF、SOD、GSH-Px水平呈现升高的趋势,MDA水平呈现降低趋势。实验24、48、72 h后,模型组、低剂量组、高剂量组大鼠缺血皮层内HSP70 mRNA阳性细胞计数、HSP70蛋白表达OD值均明显高于假手术组,且模型组、低剂量组、高剂量组缺血皮层内HSP70 mRNA阳性细胞计数、HSP70蛋白表达OD值呈现升高的趋势,差异具有统计学意义(P<0.05)。结论:丹参酚酸A可以有效地对脑缺血再灌注损伤大鼠的自由基进行清除,减少梗死面积,增加HSP70蛋白基因的表达,具有较好的缺血脑保护作用。
Abstract:
Objective:To explore the effects of salvianolic acid A on neurological function and heat shock protein gene expression in rats with cerebral ischemia,and to provide references for the study of brain protection mechanisms.Methods:80 healthy adult male SD rats were selected as the research objects.The arterial ischemia-reperfusion injury model was prepared by the suture method.According to the random number method,it was divided into 4 groups,sham operation group,model group,low-dose group and high-dose group,with 20 rats in each group.The cerebral infarction volume was compared 24 h after experiment,and the levels of glial derived neurotrophic factor(GDNF),brain derived neurotrophic factor(BDNF),malondialdehyde(MDA),glutathione peroxidase(GSH-Px)and peroxide dismutase(SOD)were compared 72 h after experiment.The changes of neurobehavioral scores after 24 h,48 h and 72 h were analyzed.The levels of HSP70 protein and HSP mRNA were measured by immunohistochemical staining and in situ hybridization after 24 h,48 h and 72 h reperfusion.Results:The cerebral infarction area in low-dose group and high-dose group was lower than that in model group,and the cerebral infarction volume in high-dose group was significantly lower.After 24 h,48 h and 72 h,the neurobehavioral scores of rats in low-dose group and high-dose group were lower than those in model group,and the neurobehavioral scores of rats in high-dose group were significantly lower.The levels of BDNF,GDNF and MDA in model group,low-dose group and high-dose group were significantly higher than those in sham group,while the levels of SOD and GSH-Px were significantly lower than those in sham group.The levels of BDNF,GDNF,SOD and GSH-Px in brain tissues of model group,low-dose group and high-dose group showed a trend of gradual increase,while the level of MDA showed a trend of gradual decrease.After 24 h,48 h and 72 h,the HSP70 mRNA positive cell count and HSP70 protein expression in ischemic cortex of rats in model group,low-dose group and high-dose group were significantly higher than those in sham group.The number of HSP70 mRNA positive cells and the expression of HSP70 protein in the ischemic cortex of model group,low-dose group and high-dose group showed a trend of increasing(P<0.05).Conclusion:Salvianolic acid A can effectively scavenge free radicals in rats with cerebral ischemia-reperfusion injury,reduce the area of infarction,increase the expression of HSP70 protein gene,and have a better protective effect on the ischemic brain.

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备注/Memo

备注/Memo:
基金项目:湖北省卫生健康委员会重点项目(WJ2019H418)
更新日期/Last Update: 2022-11-09