[1]刘锟荣,卢 洁,秦百君,等.清解化攻汤对急性胰腺炎大鼠肠黏膜屏障和血清炎症因子的影响[J].陕西中医,2023,(2):149-153.[doi:DOI:10.3969/j.issn.1000-7369.2023.02.003]
　LIU Kunrong,LU Jie,QIN Baijun,et al.Effects of Qingjie Huagong decoction on intestinal mucosal barrier and serum inflammatory factors in rats with acute pancreatitis[J].,2023,(2):149-153.[doi:DOI:10.3969/j.issn.1000-7369.2023.02.003]
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清解化攻汤对急性胰腺炎大鼠肠黏膜屏障和血清炎症因子的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年2期
页码:
149-153
栏目:
基础研究
出版日期:
2023-02-05

文章信息/Info

Title:
Effects of Qingjie Huagong decoction on intestinal mucosal barrier and serum inflammatory factors in rats with acute pancreatitis
作者:
刘锟荣1; 2; 3; 卢 洁2; 秦百君1; 安晓霞1; 2; 李 慧1; 唐曦平4; 陈国忠2
(1.广西中医药大学研究生院,广西 南宁 530001; 2.广西中医药大学第一附属医院,广西 南宁 530023; 3.广西中医药防治医学分子生物重点实验室,广西 南宁 530023; 4.广西医科大学附属肿瘤医院,广西 南宁 530021)
Author(s):
LIU Kunrong; LU Jie; QIN Baijun; AN Xiaoxia; LI Hui; TANG Xiping; CHEN Guozhong
(Graduate School of Guangxi University of Chinese Medicine,Nanning 530001,China)
关键词:
急性胰腺炎;  清解化攻汤;  肠黏膜屏障;  炎症因子;  大鼠;  肿瘤坏死因子-α
Keywords:
Acute pancreatitis;  Qingjie Huagong decoction;  Intestinal mucosal barrier;  Inflammatory factors;  Rats;  Tumor necrosis factor-α
分类号:
R 657.51
DOI:
DOI:10.3969/j.issn.1000-7369.2023.02.003
文献标志码:
A
摘要:
目的:探讨清解化攻汤对急性胰腺炎(AP)大鼠肠黏膜屏障及血清炎症因子的干预作用。方法:24只SD大鼠随机分成假造模组、模型组、西药组及中药组,每组各6只,运用雨蛙素联合脂多糖腹腔注射建立大鼠AP模型,成模后按分组给予相应药物干预,观察回肠组织的病理改变情况; 检测血清淀粉酶、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-10(IL-10)、D-乳酸及内毒素水平; Western blot检测回肠组织紧密连接蛋白Occludin的表达情况。结果:与假造模组比较,模型组回肠组织病理损伤明显,回肠黏膜绒毛略不规整或破损,肠上皮下间隙稍见增宽,与固有层分离增大,局部炎性细胞浸润增多,内皮下及固有层充血及出血明显增多,模型组血清淀粉酶、TNF-α、IL-1β、IL-10、D-乳酸及内毒素水平明显升高(P<0.01),回肠组织Occludin蛋白相对表达量明显下降; 与模型组比较,西药组和中药组病理改变明显减轻,血清淀粉酶、TNF-α、IL-1β、D-乳酸及内毒素水平均明显下降(P<0.01),血清IL-10及回肠Occludin蛋白相对表达量均明显增高(P<0.01); 与西药组比较,中药组血清淀粉酶、D-乳酸及内毒素水平降幅更明显(P<0.05)。结论:清解化攻汤对AP大鼠的肠黏膜屏障有一定的保护作用,而且该方可降低血清促炎细胞因子水平,提高抑炎细胞因子水平,对AP大鼠血清炎症因子有明显的调控作用。
Abstract:
Objective:To study the intervention effect of Qingjie Huagong decoction on intestinal mucosal barrier and serum inflammatory factors in acute pancreatitis(AP)rats.Methods:A total of 24 SD rats were divided into 4 groups in random including a fake model group,a model group,a western medicine group and a Chinese medicine group,with 6 rats in each group.The rat AP model was established by intraperitoneal injection of cerulein combined with lipopolysaccharide and immediately after the mold was formed,corresponding drug interventions were given in groups.The pathological changes of ileum tissue were observed.Serum amylase,TNF-α,IL-1β,IL-10,D-lactic acid and endotoxin levels were detected.Western blot was used to detect the expression of tight junction protein Occludin in ileum tissue.Results:In contrast with the fake model group,the model group showed more obvious pathological damage to the ileum tissue,slightly irregular or damaged ileal mucosal villi,slightly widened intestinal subepithelial space,increased separation from the lamina propria,increased local inflammatory cell infiltration and the subendothelial and lamina propria congestion and bleeding increased significantly.The serum levels of amylase,TNF-α,IL-1β,IL-10,D-lactic acid and endotoxin in the model group were significantly increased(P<0.01)and the relative expression of Occludin protein in the ileum tissue was significantly declined compared to the fake model group.Compared with the model group,the pathological changes in the western medicine group and the Chinese medicine group were significantly reduced,and the levels of serum amylase,TNF-α,IL-1β,D-lactic acid and endotoxin were significantly decreased(P<0.01).The level of serum IL-10 and the relative expression level of ileal Occludin were significantly rose compared to the model group(P<0.01).Compared with the western medicine group,the serum amylase,D-lactic acid and endotoxin levels in the Chinese medicine group decreased more significantly(P<0.05).Conclusion:Qingjie Huagong decoction has a certain protective effect on the intestinal mucosal barrier of AP rats,and the formula can reduce the level of serum pro-inflammatory cytokines,increase the level of anti-inflammatory cytokines,and has a significant regulatory effect on serum inflammatory factors in AP rats.

参考文献/References:

[1] 杜奕奇,陈其奎,李宏宇,等.中国急性胰腺炎诊治指南(2019年,沈阳)[J].临床肝胆病杂志,2019,35(12):2706-2711.
[2] Boxhoorn L,Voermans RP,Bouwense SA,et al.Acute pancreatitis[J].Lancet,2020,396(10252):726-734.
[3] Li XY,He C,Zhu Y,et al.Role of gut microbiota on intestinal barrier function in acute pancreatitis[J].World J Gastroenterol,2020,26(18):2187-2193.
[4] 陈 辉,兰 涛.肠黏膜屏障功能障碍在重症急性胰腺炎中的研究进展[J].中华实验和临床感染病杂志:电子版,2016,10(6):659-662.
[5] 吴 瑕,陈国忠,彭飞燕,等.清解化攻法治疗急性胰腺炎验案举隅[J].辽宁中医杂志,2020,47(7):160-163.
[6] 郑日辉,陈国忠,唐曦平,等.清解化攻汤结合西医常规疗法治疗重症急性胰腺炎早期合并胆石症(胆总管结石)临床研究[J].国际中医中药杂志,2022,44(2):145-149.
[7] 杨成宁,刘礼剑,刘锟荣,等.清解化攻方辅助治疗瘀毒互结型高脂血症性急性胰腺炎的临床观察[J].广西医学,2021,43(24):2928-2932.
[8] Working Group IAP/APA Acute Pancreatitis Guidelines.IAP/APA evidence-based guidelines for the management of acute pancreatitis[J].Pancreatology,2013,13(4):1-15.
[9] Guo ZZ,Wang P,Yi ZH,et al.The crosstalk between gut inflammation and gastrointestinal disorders during acute pancreatitis[J].Curr Pharm Des,2014,20(7):1051-1062.
[10] Liu J,Huang L,Luo M,et al.Bacterial translocation in acute pancreatitis[J].Crit Rev Microbiol,2019,45(5):539-547.
[11] Jakkampudi A,Jangala R,Reddy BR,et al.NF-κB in acute pancreatitis:Mechanisms and therapeutic potential[J].Pancreatology,2016,16(4):477-488.
[12] 王娜娜,刘庆民.TNF-α、IL-6、NF-κB及其抑制剂与急性胰腺炎研究进展[J].中华消化病与影像杂志:电子版,2015,5(5):253-255.
[13] 余丹青,鲁海燕,呼帮传,等.乌司他丁治疗重症急性胰腺炎的疗效及可能机制[J].中国临床药学杂志,2017,26(5):283-287.
[14] He HW,Zhang H.The efficacy of different doses of ulinastatin in the treatment of severe acute pancreatitis[J].Ann Palliat Med,2020,9(3):730-737.
[15] Wang LZ,Luo MY,Zhang JS,et al.Effect of ulinastatin on serum inflammatory factors in Asian patients with acute pancreatitis before and after treatment:A meta-analysis[J].Int J Clin Pharmacol Ther,2016,54(11):890-898.
[16] 姜志明,鲁召欣,张 明,等.乌司他丁对重症急性胰腺炎患者肠道黏膜屏障功能的影响[J].中国医药导报,2011,8(13):16-17,21.
[17] 田永超.脓毒症肠黏膜屏障功能障碍防治研究进展[J].陕西医学杂志,2020,49(4):510-513.
[18] Zhang M,Zhu HM,He F,et al.Association between acute pancreatitis and small intestinal bacterial overgrowth assessed by hydrogen breath test[J].World J Gastroenterol,2017,23(48):8591-8596.
[19] Ghosh SS,Wang J,Yannie PJ,et al.Intestinal barrier dysfunction,lps translocation,and disease development[J].J Endocr Soc,2020,4(2):39.
[20] He W,Wang Y,Wang P,et al.Intestinal barrier dysfunction in severe burn injury[J].Burns Trauma,2019,7:24.
[21] Wang B,Lin W.Edaravone protects against pancreatic and intestinal injury after acute pancreatitis via nuclear factor-κB signaling in mice[J].Biol Pharm Bull,2020,43(3):509-515.
[22] Zhang XM,Zhang ZY,Zhang CH,et al.Intestinal microbial community differs between acute pancreatitis patients and healthy volunteers[J].Biomed Environ Sci,2018,31(1):81-86.
[23] Deng WS,Zhang J,Ju H,et al.Arpin contributes to bacterial translocation and development of severe acute pancreatitis[J].World J Gastroenterol,2015,21(14):4293-4301.
[24] 杜 姗,卜 爱.肠内营养治疗在重症监护室重症急性胰腺炎治疗中的临床研究[J].陕西医学杂志,2019,48(7):913-915.
[25] 马明和,刘永萍,刘 萍,等.攻下清热活血方对重症急性胰腺炎大鼠血清相关炎性因子及肠黏膜屏障功能的影响研究[J].陕西中医,2020,41(8):1051-1054.
[26] Palathingal BE,George J,Tarique M,et al.Pirfenidone increases IL-10 and improves acute pancreatitis in multiple clinically relevant murine models[J].JCI Insight,2022,7(2):141108.
[27] 季华庆,杨 平.血必净注射液联合乌司他丁对重症急性胰腺炎患者临床疗效及对血清炎症因子水平的影响[J].陕西中医,2017,38(7):909-910.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82160890); 广西壮族自治区自然科学基金资助项目(2019JJA140220); 广西中医药大学研究生教育创新计划项目(YCBSY2020008); 广西中医药大学第一附属医院院级科研项目(2019QN001)
更新日期/Last Update: 2023-02-08