[1]黄昌锐,李汶珊,袁 惠,等.护心康片对动脉粥样硬化小鼠自噬干预机制[J].陕西中医,2023,(7):854-858.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.006]
　HUANG Changrui,LI Wenshan,YUAN Hui,et al.Intervention mechanism of cardiac protection tablets on autophagy in atherosclerotic mice[J].,2023,(7):854-858.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.006]
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护心康片对动脉粥样硬化小鼠自噬干预机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年7期
页码:
854-858
栏目:
基础研究
出版日期:
2023-07-05

文章信息/Info

Title:
Intervention mechanism of cardiac protection tablets on autophagy in atherosclerotic mice
作者:
黄昌锐1; 李汶珊1; 袁 惠1; 喻正科2
(1.湖南省中医药研究院,湖南 长沙 410006; 2.湖南省中医药研究院附属医院,湖南 长沙410006)
Author(s):
HUANG Changrui; LI Wenshan; YUAN Hui; YU Zhengke
(Hunan Academy of Chinese Medicine,Changsha 410006,China)
关键词:
动脉粥样硬化;  护心康片;  磷脂酰肌醇3激酶;  蛋白激酶B;  雷帕霉素靶蛋白;  自噬
Keywords:
Atherosclerosis;  Cardiac protection tablets;  Phosphatidylinositol 3 kinase;  Protein kinase B;  Mammalian target of rapamyc;  Autophagy
分类号:
R 543.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.07.006
文献标志码:
A
摘要:
目的:探讨护心康片调节磷脂酰肌醇3激酶/蛋白激酶B/雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路干预动脉粥样硬化小鼠自噬的机制。方法:采用高脂饲料喂养,载脂蛋白E基因敲除(ApoE-/-)小鼠建立动脉粥样硬化(AS)模型,以C57BL/6J小鼠给予普通饲料喂养作为空白组。造模成功后ApoE-/-小鼠随机分为模型组、护心康片低剂量组、护心康片中剂量组、护心康片高剂量组、阿托伐他汀组。护心康片各组分别按0.6、1.2、2.4 g/(kg·d)给予护心康片,阿托伐他汀组按1.3 mg/(kg·d)给予阿托伐他汀钙片,均连续干预12周。HE染色观察小鼠主动脉病理变化,全自动生化分析仪检测血清TC、TG、LDL-C水平,RT-qPCR法检测Akt、mTOR、Beclin-1、LC3-Ⅱ的mRNA表达水平,Western blotting法检测Akt、mTOR、Beclin-1、LC3-Ⅱ蛋白表达。结果:治疗后,护心康片低剂量组、护心康片中剂量组、护心康片高剂量组斑块面积小于模型组,且血清TG、TC、LDL-C低于模型组,差异有统计学意义(均P<0.05)。护心康片低剂量组、护心康片中剂量组、护心康片高剂量组Akt、mTOR、Beclin-1、LC3-Ⅱ的mRNA及蛋白表达高于模型组,差异有统计学意义(均P<0.05)。结论:护心康片对ApoE-/-小鼠动脉粥样硬化斑块形成有干预作用,其机制可能与调控PI3K/Akt/mTOR信号通路、激活自噬有关。
Abstract:
Objective:To investigate effect of cardiac protection tablets on autophagy in atherosclerotic mice by regulating phosphatidylinositol 3 kinase/ protein kinase B/ mammalian target of rapamyc(PI3K/Akt/mTOR)signaling pathway.Methods:Apolipoprotein E gene knockout(ApoE-/-)mice were fed high-fat chow to establish AS model,and C57BL/6J mice fed normal chow as the blank group.After successful modeling,ApoE-/- mice were randomly divided into the model group,the low cardiac protection group,the medium cardiac protection group,the high cardiac protection groups,the atorvastatin group.The dose of cardiac protection tablets were administered to each dose group of cardiac protection for 0.6,1.2,2.4 g/(kg·d)and the atorvastatin group given atorvastatin calcium 1.3 mg/(kg·d),all groups intervening for 12 weeks.The pathological changes of mouse aorta were observed by HE staining,serum TC,TG and LDL-C levels detected by automatic biochemical analyzer,mRNA expression levels of Akt,mTOR,Beclin-1 and LC3-Ⅱ detected by RT-qPCR,and Akt,mTOR,Beclin-1 and LC3-Ⅱ detected by Western blotting.Results:After treatment,compared with the model group,the plaque area was significantly reduced in the low cardiac protection group,the medium cardiac protection group,the high cardiac protection groups,including the serum TG,TC and LDL-C levels reduced,difference statistically significant(all P<0.05).The mRNA and protein expression of Akt,mTOR,Beclin-1 and LC3-Ⅱ were higher than those in the model group,difference statistically significant(all P<0.05).Conclusion:Cardiac protection tablets have intervention effect on atherosclerotic plaque formation in ApoE-/- mice.The mechanism may be related to activation of autophagy by regulating PI3K/Akt/mTOR signaling pathway.

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备注/Memo

备注/Memo:
基金项目:湖南省中医药科研计划重点项目(202009)
更新日期/Last Update: 2023-07-10