[1]王 琪,吴 限,刘 丰,等.黄连温胆汤通过PI3K/Akt通路对缺氧复氧诱导心肌细胞损伤作用机制[J].陕西中医,2023,(7):859-863.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.007]
　WANG Qi,WU Xian,LIU Feng,et al.Impacts and mechanism of Huanglian Wendan decoction on myocardial cell injury induced by hypoxia reoxygenation through PI3K/Akt pathway[J].,2023,(7):859-863.[doi:DOI:10.3969/j.issn.1000-7369.2023.07.007]
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黄连温胆汤通过PI3K/Akt通路对缺氧复氧诱导心肌细胞损伤作用机制
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年7期
页码:
859-863
栏目:
基础研究
出版日期:
2023-07-05

文章信息/Info

Title:
Impacts and mechanism of Huanglian Wendan decoction on myocardial cell injury induced by hypoxia reoxygenation through PI3K/Akt pathway
作者:
王 琪; 吴 限; 刘 丰; 苗永悦; 孙少谦; 刘 莉
(黑龙江中医药大学附属第一医院,黑龙江 哈尔滨 150040)
Author(s):
WANG Qi; WU Xian; LIU Feng; MIAO Yongyue; SUN Shaoqian; LIU Li
(First Affiliated Hospital,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
关键词:
黄连温胆汤;  磷脂酰肌醇3激酶/丝氨酸苏氨酸蛋白激酶通路;  缺氧复氧;  心肌损伤;  凋亡;  氧化应激
Keywords:
Huanglian Wendan decoction;  Phosphoinositide-3-kinase/serine threonine protein kinase pathway;  Hypoxia reoxygenation;  Myocardial injury;  Apoptosis;  Oxidative stress
分类号:
R 289.5
DOI:
DOI:10.3969/j.issn.1000-7369.2023.07.007
文献标志码:
A
摘要:
目的:探讨黄连温胆汤通过磷脂酰肌醇3激酶(PI3K)/丝氨酸苏氨酸蛋白激酶(Akt)通路对缺氧复氧诱导心肌细胞损伤的作用机制。方法:构建缺氧复氧诱导的大鼠心肌细胞H9C2损伤模型,分为对照组、缺氧复氧组、黄连温胆汤低剂量组、黄连温胆汤中剂量组、黄连温胆汤高剂量组。CCK-8法检测H9C2细胞增殖。流式细胞术检测H9C2细胞凋亡。检测细胞内活性氧(ROS)、细胞上清中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。Western blotting检测细胞中Bcl-2相关X蛋白(Bax)、B淋巴细胞瘤-2(Bcl-2)、p-PI3K、p-Akt蛋白表达。结果:缺氧复氧组H9C2细胞OD450值、SOD活性、Bcl-2、p-PI3K、p-Akt蛋白表达均低于对照组,而细胞凋亡率、ROS平均荧光强度、MDA含量、Bax蛋白表达高于对照组,差异有统计学意义(均P<0.05)。黄连温胆汤低剂量组、黄连温胆汤中剂量组、黄连温胆汤高剂量组H9C2细胞OD450值、SOD活性、Bcl-2、p-PI3K、p-Akt蛋白表达高于缺氧复氧组,而细胞凋亡率、ROS平均荧光强度、MDA含量、Bax蛋白表达低于缺氧复氧组,差异有统计学意义(均P<0.05)。结论:黄连温胆汤通过激活PI3K/Akt信号通路减轻缺氧复氧诱导的H9C2细胞损伤。
Abstract:
Objective:To investigate impact and mechanism of Huanglian Wendan decoction on myocardial cell injury induced by hypoxia reoxygenation through phosphatidylinositol-3-kinase(PI3K)/serine threonine protein kinase(Akt)pathway.Methods:A model of H9C2 injury in rat cardiomyocytes induced by hypoxia reoxygenation was established and grouped into control group,hypoxia reoxygenation group,low dose Huanglian Wendan decoction group,medium dose Huanglian Wendan decoction group,high dose Huanglian Wendan decoction group.CCK-8 method was used to detect proliferation of H9C2 cells.The apoptosis of H9C2 cells was detected by flow cytometry.Intracellular reactive oxygen species(ROS),superoxide dismutase(SOD)activity and malondialdehyde(MDA)content in cell supernatant were detected.Western blotting was applied to detect expression of Bcl-2 associated X protein(Bax),B cell-lymphomatoma-2(Bcl-2),p-PI3K,and p-Akt proteins in the cells.Results:The OD450 value,SOD activity,protein expression of Bcl-2,p-PI3K and p-Akt in H9C2 cells in the hypoxia reoxygenation group were lower than those in the control group,and apoptosis rate,ROS average fluorescence intensity,content of MDA,and protein expression of Bax were higher,difference statistically significant(all P<0.05).The OD450 value,SOD activity,protein expression of Bcl-2,p-PI3K and p-Akt in H9C2 cells in the low,medium,and high dose Huanglian Wendan decoction groups were higher than those in the hypoxia reoxygenation group,while the apoptosis rate,ROS average fluorescence intensity,content of MDA,and protein expression of Bax lower,difference statistically significant(all P<0.05).Conclusion:Huanglian Wendan decoction may alleviate injury of H9C2 cells induced by hypoxia reoxygenation by activating the PI3K/Akt signaling pathway.

参考文献/References:

[1] 马瑞聪,丁彦春. 急性心肌梗死后应激性高血糖的研究进展[J].心血管病学进展,2023,44(3):198-202.
[2] 姚 娜,张裕珍.血府逐瘀汤对急性心肌梗死患者的影响[J].光明中医,2023,38(8):1511-1513.
[3] 陈 靖,肖 珉,李 攀,等.黄连温胆汤加减治疗心悸(室性早搏)痰火扰心证的中医证候改善及预后分析[J].四川中医,2021,39(1):54-57.
[4] 李博文.加味黄连温胆汤对冠心病稳定型心绞痛患者疗效及免疫因子的影响[J].医学理论与实践,2021,34(7):1131-1132.
[5] 李晓玲,王 丹,肖 扬,等.左西孟旦对缺氧复氧诱导的H9c2细胞凋亡、PTEN和PI3K/Akt信号通路的影响[J].中国动脉硬化杂志,2022,30(11):942-948.
[6] 马伯艳,辛相如,陆阁玲,等.基于NLRP3/Caspase-1信号通路探讨黄连温胆汤改善HepG2细胞胰岛素抵抗的作用机制[J].中国实验方剂学杂志,2022,28(18):1-11.
[7] 张生财,朱卫喜,江泽宇,等.积雪草酸对缺氧/复氧所致心肌细胞损伤的保护作用[J].沈阳药科大学学报,2022,39(10):1245-1250.
[8] 张云波,顾申红,陈菊明,等.基于RhoA/ROCK信号通路探讨四逆汤对心肌缺血再灌注氧化损伤作用机制[J].陕西中医,2022,43(3):301-304.
[9] 黄大道.中医辨证治疗急性心肌梗死的临床效果[J].内蒙古中医药,2021,40(6):55-56.
[10] 杨金果,鞠建庆,汤献文,等.黄连温胆汤调控NLRP3炎症小体抗动脉粥样硬化机制[J].中国老年学杂志,2022,42(19):4729-4733.
[11] 黄 琪,隋向前,王士生,等.黄连温胆汤对痰热瘀阻型原发性微血管心绞痛患者内皮功能的影响[J].新中医,2022,54(18):29-34.
[12] 陈智勇,车 驰,王晓琳,等.基于网络药理学探讨黄连温胆汤治疗心律失常的机制[J].现代药物与临床,2022,37(8):1718-1730.
[13] 杨金果,鞠建庆,汤献文,等.黄连温胆汤调控PI3K/Akt/mTOR信号通路干预ApoE-/-小鼠动脉粥样硬化的作用机制[J].中西医结合心脑血管病杂志,2022,20(4):620-626.
[14] 吴 怡,马晓旭.黄连温胆汤治疗冠心病稳定型心绞痛的疗效分析[J].现代医学与健康研究电子杂志,2021,5(22):74-76.
[15] 杨 伟,王 雁,李俊杰,等.miR-499a-5p通过靶向TLR2抑制缺氧/复氧诱导的心肌细胞氧化应激和凋亡[J].川北医学院学报,2022,37(10):1239-1244.
[16] 李 想,李安特,孙秋霞,等.PI3K/Akt信号通路与神经损伤修复的研究进展[J].中国老年学杂志,2023,43(1):246-249.
[17] 任倪辉,郭志佳,王 鑫,等.七氟醚上调PI3K/Akt/mTOR活性对小鼠心肌缺血再灌注损伤中自噬水平的影响[J].武警医学,2022,33(5):416-419.
[18] 付华君,孟 娟,宋文英,等.紫檀芪调节PI3K/Akt/mTOR信号通路保护心肌缺血再灌注损伤大鼠的作用机制研究[J].中西医结合心脑血管病杂志,2022,20(7):1227-1231.
[19] 王 飘,郑 晴,胡 婷,等.基于PI3K/Akt/mTOR信号通路的三七总皂苷调控自噬抗H9c2细胞缺氧/复氧损伤机制研究[J].中国中医药信息杂志,2021,28(8):87-92.
[20] 何妙嬴,张燚格,柳冬冬,等.电针内关预处理对心肌缺血再灌注损伤大鼠心肌PI3K/Akt通路的影响[J].陕西中医,2023,44(3):298-303.

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备注/Memo

备注/Memo:
基金项目:黑龙江省中医药管理局科研项目(ZHY2023-183); 第七批全国老中医药专家学术经验继承工作项目[国中医药人教函(2022)76号]
更新日期/Last Update: 2023-07-10