[1]张 驰,李奥博,王心怡,等.温阳振衰颗粒调控miR-155/p38MAPK途径减轻大鼠心肌缺血再灌注损伤机制研究[J].陕西中医,2023,(8):1005-1009.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.003]
　ZHANG Chi,LI Aobo,WANG Xinyi,et al.Wenyang Zhenshuai granule reduce myocardial ischemia-reperfusion injury in rats via regulating miR-155/p38MAPK pathway[J].,2023,(8):1005-1009.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.003]
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温阳振衰颗粒调控miR-155/p38MAPK途径减轻大鼠心肌缺血再灌注损伤机制研究
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年8期
页码:
1005-1009
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Wenyang Zhenshuai granule reduce myocardial ischemia-reperfusion injury in rats via regulating miR-155/p38MAPK pathway
作者:
张 驰1; 李奥博2; 王心怡1; 王沛昕3
(1.北京大学第三医院,北京 100621; 2.北京丰台右安门医院,北京 100069; 3.北京丰台医院,北京 100071)
Author(s):
ZHANG Chi; LI Aobo; WANG Xinyi; WANG Peixin
(Peking University Third Hospital,Beijing 100621,China)
关键词:
心肌缺血再灌注损伤;  温阳振衰颗粒;  miR-155;  p38MAPK;  炎症因子;  心肌酶
Keywords:
Myocardial ischemia-reperfusion injury;  Wenyang Zhenshuai granule;  miR-155;  p38MAPK;  Inflammation factors;  Myocardial enzyme
分类号:
R 542
DOI:
DOI:10.3969/j.issn.1000-7369.2023.08.003
文献标志码:
A
摘要:
目的:研究温阳振衰颗粒调控miR-155/p38MAPK途径减轻大鼠心肌缺血再灌注损伤(MIRI)的作用机制。方法:成年雄性SD大鼠随机分为阴性对照组、阴性对照+MIRI组、miR-155抑制剂+MIRI组、miR-155模拟物+MIRI组、对照组、MIRI组、中药组、中药+miR-155抑制剂组,建立MIRI模型,给予温阳振衰颗粒灌胃干预,miR-155抑制剂或模拟物局部注射。比较各组磷酸肌酸激酶同工酶(CK-MB)及乳酸脱氢酶(LDH)含量、miR-155及p-p38MAPK表达、炎症因子肿瘤坏死因子-α(TNF-α)及白介素-6(IL-6)含量。结果:与对照组比较,MIRI组血清CK-MB、LDH含量及心肌p-p38MAPK表达、TNF-α、IL-6含量明显增加,心肌miR-155表达明显减少(均P<0.05); 与MIRI组比较,中药组血清CK-MB、LDH含量及心肌p-p38MAPK表达、TNF-α、IL-6含量明显减少,心肌miR-155表达明显增加(均P<0.05); 与中药组比较,中药+miR-155抑制剂组血清CK-MB、LDH含量及心肌p-p38MAPK表达、TNF-α、IL-6含量明显增加,心肌miR-155表达明显减少(均P<0.05); 与阴性对照+MIRI组比较,miR-155抑制剂+MIRI组心肌损伤加重,miR-155模拟物+MIRI组心肌损伤减轻(均P<0.05)。结论:温阳振衰颗粒通过调控miR-155/p38MAPK途径减轻大鼠MIRI,miR-155能够在心肌MIRI过程中靶向p38MAPK,抑制炎症并减轻心肌损伤。
Abstract:
Objective:To study the effect and mechanism of Wenyang Zhenshuai granule on reducing myocardial ischemia-reperfusion injury(MIRI)via regulating miR-155/p38MAPK pathway.Methods:Adult male SD rats were randomly divided into control group,MIRI group,TCM group,TCM+miR-155 inhibitor group,negative control(NC)group,NC+MIRI group,miR-155 inhibitor+MIRI group,miR-155 mimic+MIRI group.The contents of CK-MB,LDH,the expression of miR-155,p-p38MAPK,the contents of TNF-α and IL-6 were compared among the groups.Results:Compared with the control group,the contents of CK-MB and LDH in serum and the expression of p-p38MAPK,the contents of TNF-α and IL-6 in myocardium of MIRI group significantly increased,and the expression of miR-155 in myocardium significantly decreased(all P<0.05).Compared with MIRI group,the contents of CK-MB and LDH in serum and the expression of p-p38MAPK,the contents of TNF-α and IL-6 in myocardium of TCM group significantly decreased,and the expression of miR-155 in myocardium significantly increased(all P<0.05).Compared with TCM group,the contents of CK-MB and LDH in serum and the expression of p-p38MAPK,the contents of TNF-α and IL-6 in myocardium of TCM+miR-155 inhibitor group significantly increased,and the expression of miR-155 in myocardium significantly decreased(all P<0.05).Compared with NC+MIRI group,myocardial injury was aggravated in miR-155 inhibitor+MIRI group,and myocardial injury was alleviated in miR-155 mimic+MIRI group(all P<0.05).Conclusion:Wenyang Zhenshuai granule can reduce MIRI by regulating miR-155/p38MAPK pathway and miR-155 can inhibit inflammation and reduce injury during MIRI by targeting p38MAPK.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81574052)
更新日期/Last Update: 2023-08-10