[1]霍新慧,周知然,韦 芳,等.针灸预处理对急性胃黏膜损伤大鼠GRP78/Caspase-12/CHOP信号通路的影响[J].陕西中医,2023,(8):1015-1020.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.005]
　HUO Xinhui,ZHOU Zhiran,WEI Fang,et al.Effect of acupuncture pretreatment on GRP78/Caspase-12/CHOP signaling pathway in rats with acute gastric mucosal lesion[J].,2023,(8):1015-1020.[doi:DOI:10.3969/j.issn.1000-7369.2023.08.005]
点击复制

针灸预处理对急性胃黏膜损伤大鼠GRP78/Caspase-12/CHOP信号通路的影响
分享到：

《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年8期
页码:
1015-1020
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effect of acupuncture pretreatment on GRP78/Caspase-12/CHOP signaling pathway in rats with acute gastric mucosal lesion
作者:
霍新慧1; 周知然2; 韦 芳1; 李 盼1; 兰永利1
(1.新疆医科大学中医学院,新疆 乌鲁木齐 830001; 2.新疆维吾尔自治区中医医院消化科,新疆 乌鲁木齐 830099)
Author(s):
HUO Xinhui; ZHOU Zhiran; WEI Fang; LI Pan; LAN Yongli
(College of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830001,China)
关键词:
胃黏膜损伤;  针刺预处理;  艾灸预处理;  内质网应激;  炎症因子;  大鼠
Keywords:
Gastric mucosal lesion;  Acupuncture petreatment;  Moxibustion pretreatment;  Endoplasmic reticulum stress;  Inflammatory factor;  Rat
分类号:
R 573
DOI:
DOI:10.3969/j.issn.1000-7369.2023.08.005
文献标志码:
A
摘要:
目的:探讨针灸预处理对急性胃黏膜损伤大鼠葡萄糖调节蛋白78(GRP78)/半胱氨酸蛋白水解酶-12(Caspase-12)/CCAAT增强子结合蛋白同源蛋白(CHOP)信号通路的影响。方法:将52只大鼠随机分为正常组、模型组、艾灸预处理组、针刺预处理组,每组13只。正常组、模型组仅固定不作处理; 对针刺预处理组大鼠行针刺处理; 对艾灸预处理组大鼠行足三里穴、中脘穴艾灸,20 min/次,1次/d,连续灸7 d。对模型组、针刺预处理组、艾灸预处理组大鼠进行无水乙醇结合阿司匹林混悬液灌胃建立急性胃黏膜损伤模型。观察大鼠一般情况及胃黏膜损伤组织病理学变化; 酶联免疫吸附实验(ELISA)法检测大鼠血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、环氧化酶-2(COX-2)浓度; 免疫组织化学法检测大鼠GRP78、Caspase-12、CHOP水平。结果:模型组大鼠可见上皮细胞结构破坏不完整,胃黏膜组织损伤明显,胃黏膜损伤指数(UI)评分显著高于正常组(P<0.05); 与模型组比较,针刺预处理组、艾灸预处理组大鼠UI指数评分显著降低(均P<0.05)。与正常组比较,模型组大鼠血清TNF-α、IL-6、COX-2浓度显著升高(均P<0.05); 与模型组比较,针刺预处理组、艾灸预处理组大鼠血清TNF-α、IL-6、COX-2浓度显著降低(均P<0.05)。与正常组比较,模型组大鼠GRP78、Caspase-12、CHOP呈强阳性表达,可见大量棕黄色颗粒,针刺预处理组、艾灸预处理组大鼠GRP78、Caspase-12、CHOP结果阳性细胞数量减少,呈弱阳性,可见少量棕黄色颗粒。结论:针灸预处理可减轻急性胃黏膜损伤大鼠炎症反应,其作用机制可能是通过抑制内质网应激,基于GRP78/Caspase-12/CHOP信号通路预防大鼠胃黏膜损伤。
Abstract:
Objective:To investigate the effects of acupuncture pretreatment on the histopathology of gastric mucosa and glucose-regulated protein 78(GRP78)/cysteine protein hydrolase-12(Caspase-12)/CCAAT enhancer-binding protein homologous protein(CHOP)signaling pathways in rats with acute gastric mucosal lesion.Methods:52 rats were randomly divided into normal group,model group,moxibustion pretreatment group,acupuncture pretreatment group,each group consisted of 13 rats.Normal group and model group were fixed without treatment.The rats in the acupuncture pretreatment group were treated with acupuncture.The rats in the moxibustion pretreatment group were subjected to moxibustion at the Zusanli point and the Zhongwan point,20 min each time,moxibustion once a day for 7 days.After pretreatment,rats in the model group,moxibustion pretreatment group and acupuncture pretreatment group were gavaged with anhydrous ethanol combined with aspirin suspension to establish a model of acute gastric mucosal lesion.The general condition of rats and histopathological changes of gastric mucosal injury were observed.The concentrations of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and cyclooxygenase-2(COX-2)in serum of rats were determined by enzyme-linked immunosorbent assay(ELISA).The levels of GRP78,Caspase-12 and CHOP were determined by immunohistochemistry.Results:In the model group,incomplete structural destruction of epithelial cells and significant damage to gastric mucosal tissues were seen,and the UI index score was significantly higher than that of the normal group(P<0.05).Compared with model group,UI index scores of rats in acupuncture pretreatment group and moxibustion pretreatment group were significantly decreased(all P<0.05).Compared with the normal group,the levels of serum TNF-α,IL-6 and COX-2 in the model group were significantly higher(all P<0.05).Compared with the model group,the levels of serum TNF-α,IL-6 and COX-2 concentrations in the acupuncture and moxibustion pretreatment group were significantly lower(all P<0.05).Compared with the normal group,GRP78,Caspase-12 and CHOP in the gastric mucosa of rats in the model group showed strong positive expression and a large number of brownish-yellow granules were visible,while the number of GRP78,Caspase-12 and CHOP positive cells in the acupuncture pretreatment group decreased and were weakly positive,and a small number of brownish-yellow granules were visible.Conclusion:Acupuncture pretreatment can reduce inflammatory response in AGML rats,and its mechanism may be through inhibiting ERS and preventing gastric mucosal injury based on GRP78/Caspase-12/CHOP signaling pathway.

参考文献/References:

[1] 向丽婷,李 飞,刘 芳,等.从迷走神经通路研究艾灸对胃粘膜损伤修复机制的研究进展[J].针灸临床杂志,2016,32(7):84-87.
[2] 赫吉铭,霍新慧,郭梓杰,等.灸预处理对急性胃黏膜损伤大鼠胃黏膜微循环屏障相关因子作用的影响[J].新疆医科大学学报,2018,41(6):785-788.
[3] 李永芳,连亚军,谢南昌,等.香芹酚对癫痫大鼠内质网应激相关分子表达的影响[J].医药导报,2016,35(1):16-20.
[4] 张路煜,刘玉晖,游 宇,等.葛根对2型糖尿病大鼠胰腺内质网应激相关蛋白GRP78、ATF6表达的影响[J].中国实验方剂学杂志,2020,26(20):82-87.
[5] 曹智怡,李 玲,范丽丽,等.酸枣仁-合欢花对抑郁模型大鼠行为学及内质网应激PERK/ATF4/CHOP通路的影响[J].中国实验方剂学杂志,2021,27(7):66-72.
[6] 张忠胜,刘双凤,黄四春.胸腺素β4对大鼠脑缺血再灌注后内质网应激的影响[J].中南药学,2019,17(7):1005-1009.
[7] 李 莉,郑 丹,张 佳,等.内质网应激因子Caspase-12与子痫前期发病机制的关系[J].江西医药,2021,56(4):533-536.
[8] 郭 义.实验针灸学[M].北京:中国中医药出版社,2008:415.
[9] 霍新慧,智 勇,赫吉铭,等.针灸预处理对胃黏膜损伤大鼠胃黏膜微循环相关因子一氧化氮、内皮素的影响[J].吉林中医药,2018,38(12):1434-1437.
[10] 林亚平,封迎帅,彭 艳,等.艾灸预处理对幽门螺旋杆菌胃炎大鼠胃组织白细胞介素的影响[J].北京中医药大学学报,2013,36(11):757-761.
[11] 侯艳玲,林亚平,史冬梅.幽门螺杆菌感染致急性胃黏膜损伤与相关细胞因子关系的研究进展[J].中国中医急症,2012,21(9):1460-1462.
[12] 杜 燕,易受乡,林亚平,等.艾灸对急性胃黏膜损伤大鼠热休克蛋白与相关炎性细胞因子的影响[J].上海针灸杂志,2010,29(5):269-272.
[13] 毕潇文,李 静.支气管哮喘合并肺炎支原体感染患儿血清白介素-6、25羟基维生素D、肿瘤坏死因子水平变化临床研究[J].陕西医学杂志,2022,51(1):63-66.
[14] 高 洁.胃蛋白酶原、胃泌素-17在慢性萎缩性胃炎中的表达及其与幽门螺杆菌感染相关性分析[J].陕西医学杂志,2019,48(1):131-134.
[15] 黄 杨,程文静,李 鹏,等.艾灸胃经穴对慢性萎缩性胃炎大鼠胃黏膜miR-146a、NF-κBp65及COX-2表达的影响[J].中华中医药杂志,2022,37(6):3480-3483.
[16] 苏淑伶,苏 龙,郑丽丽,等.HIF-1α、COX-2、CRP及ESR在溃疡性结肠炎患者外周血及黏膜组织中的表达及临床意义[J].临床和实验医学杂志,2022,21(4):415-419.
[17] 张声生,宋 瑾,王天园,等.猴头健胃灵片对胃黏膜损伤模型大鼠胃黏膜组织中PGE2含量及COX-2蛋白表达的影响[J].中医杂志,2022,63(3):256-261.
[18] 向丽婷,彭 亮,李 飞,等.艾灸对迷走神经切断后大鼠急性胃黏膜损伤修复的研究[J].辽宁中医杂志,2017,44(1):167-170.
[19] 王园园,张国山,李 菲,等.针刺预处理对急性胃黏膜损伤大鼠EGF、TGF-α及TFF3含量的影响[J].湖南中医药大学学报,2018,38(11):1253-1257.
[20] 郭礼娜,刘薇薇,谭成富,等.艾灸、电针预处理对急性胃黏膜损伤大鼠TGF-α、PCNA的影响[J].湖南中医药大学学报,2016,36(4):45-48.
[21] 薛 翔,聂时南.肠三叶因子对胃肠道保护作用的研究进展[J].医学研究生学报,2014,27(4):439-443.
[22] 徐 盟,王 涛.内质网应激反应与相关分子伴侣[J].解剖科学进展,2014,20(4):381-384.
[23] 曹佳男,钟 欢,刘 密,等.温针灸对慢性萎缩性胃炎大鼠局部穴区组织代谢的影响[J].湖南中医药大学学报,2020,40(9):1054-1059.
[24] 王晨光,杨宗保,左彬荣,等.针刺调节急性胃黏膜损伤大鼠胃黏膜细胞增殖信号通路相关分子的表达[J].中华中医药杂志,2014,29(9):2797-2799.
[25] 张 鼎,黄烈弥,桂星花,等.冬病夏治温针灸治疗脾胃虚寒型胃痛临床研究[J].陕西中医,2021,42(4):527-530.
[26] 易受乡,杜 燕,林亚平,等.艾灸及烟条灸预处理对急性胃黏膜损伤大鼠热休克蛋白和炎性细胞因子的影响[J].中国中医药信息杂志,2011,18(4):26-28.
[27] 谢莉莉,刘光谱.艾灸的治疗作用和机理研究进展[J].针灸临床杂志,2000,16(5):57-59.
[28] 马志琳,牟方宏,崔梅花,等.马来酸伊索拉定对阿司匹林所致大鼠胃黏膜损伤的保护作用[J].胃肠病学和肝病学杂志,2016,25(5):542-546.
[29] 马晓丽,王 蕊,杨正飞,等.电针足三里穴对糖尿病胃轻瘫(DGP)大鼠肠神经胶质细胞(EGCs)的影响及其作用机制研究[J].亚太传统医药,2022,18(12):20-25.
[30] 龙 漫.足三里电针预处理对早期应激性胃黏膜损伤大鼠胃黏膜保护作用及机制研究[D].武汉:湖北中医药大学,2022.
[31] 蒋传林.泮托拉唑联合针刺足三里、中脘穴治疗慢性浅表性胃炎的效果观察[J].中国社区医师,2023,39(13):77-79.
[32] 林丽云.理中汤加减联合隔盐压灸中脘穴、至阳穴治疗脾胃虚寒型胃脘痛的效果[J].中外医学研究,2021,19(24):111-113.

相似文献/References:

[1]兰永利,周知然,韦 芳,等.针灸预处理调控TLR4/MyD88/NF-κB信号通路抗大鼠胃黏膜损伤机制研究[J].陕西中医,2023,(10):1349.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.004]
　LAN Yongli,ZHOU Zhiran,WEI Fang,et al.Mechanisms of acupuncture pretreatment in regulating TLR4/MyD88/NF-κB signaling pathway against gastric mucosal injury in rats[J].,2023,(8):1349.[doi:DOI:10.3969/j.issn.1000-7369.2023.10.004]

备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2021D01C224); 新疆医科大学第四附院院级课题(ZYY2019ZD04)
更新日期/Last Update: 2023-08-10