[1]张 硕,温 博.清热养阴除湿丸对痛风大鼠炎症因子及关节功能的影响[J].陕西中医,2023,(11):1518-1522.[doi:DOI:10.3969/j.issn.1000-7369.2023.11.005]
　ZHANG Shuo,WEN Bo.Effect of Qingre Yangyin Chushi Wan on inflammatory factors and joint function in rats with gout[J].,2023,(11):1518-1522.[doi:DOI:10.3969/j.issn.1000-7369.2023.11.005]
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清热养阴除湿丸对痛风大鼠炎症因子及关节功能的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年11期
页码:
1518-1522
栏目:
基础研究
出版日期:
2023-11-05

文章信息/Info

Title:
Effect of Qingre Yangyin Chushi Wan on inflammatory factors and joint function in rats with gout
作者:
张 硕; 温 博
(首都医科大学附属北京中医医院,北京100010)
Author(s):
ZHANG Shuo; WEN Bo
(Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China)
关键词:
痛风;  清热养阴除湿丸;  炎症小体;  线粒体自噬;  秋水仙碱;  NOD样受体蛋白3
Keywords:
Gout;  Qingre Yangyin Chushi Wan;  Inflammatory corpuscles;  Mitochondrial autophagy;  Colchicine;  NOD like receptor protein 3
分类号:
R 971.1
DOI:
DOI:10.3969/j.issn.1000-7369.2023.11.005
文献标志码:
A
摘要:
目的:探讨清热养阴除湿丸对痛风大鼠的治疗机制。方法:将50只SD大鼠随机分为空白组、模型组、秋水仙碱组、清热养阴除湿低剂量组、清热养阴除湿高剂量组。采用改良Coderre 法构建痛风大鼠模型,按组别给予大鼠0.9%氯化钠溶液、秋水仙碱、清热养阴除湿丸灌胃治疗。通过HE染色观察大鼠滑膜组织病理改变; 采用ELISA法检测大鼠外周血IL-1β、IL-18、IL-33、TNF-α水平; 采用RT-qPCR、Western blotting检测大鼠滑膜组织中NLRP3炎症小体相关基因及线粒体自噬相关基因表达。结果:模型组大鼠外周血IL-1β、IL-18、IL-33、TNF-α高于空白组(均P<0.05)。干预后,秋水仙碱组、清热养阴除湿低剂量组、清热养阴除湿高剂量组大鼠外周血IL-1β、IL-18、IL-33、TNF-α下降,且清热养阴除湿高剂量组各项指标最低(P<0.05)。清热养阴除湿低剂量组、清热养阴除湿高剂量组大鼠滑膜组织中NLRP3、Caspase-1、IL-1β、LC3B、p62、PHB2蛋白相对表达低于模型组(均P<0.05)。结论:清热养阴除湿丸通过抑制NLRP3炎症小体形成及线粒体相关自噬的发生减轻痛风大鼠滑膜组织炎症,改善关节功能。
Abstract:
Objective:To explore therapeutic mechanism of Qingre Yangyin Chushi Wan on gout rats.Methods:Fifty SD rats were randomly divided into blank group,model group,colchicine group,Qingre Yangyin Chushi low-dose group and Qingre Yangyin Chushi high-dose group.The gout rat model were constructed using improved coderre method,and given 0.9% sodium chloride solution,colchicine,Qingre Yangyin Chushi Wan by intragastric administration.The pathological changes of synovial tissue were observed by HE staining.The levels of IL-1β,IL-18,IL-33,TNF-α in peripheral blood of rats were detected by ELISA.RT-qPCR and Western blotting were used to detect expression of NLRP3 inflammator-related genes and mitochondrial autophagy related genes in the synovial tissues of rats.Results:IL-1β,IL-18,IL-33 and TNF-α in peripheral blood of rats in the model group were higher than those in the blank group(all P<0.05).After intervention,IL-1β,IL-18,IL-33 and TNF-α in peripheral blood of rats in the colchicine group,Qingre Yangyin Chushi low-dose group and Qingre Yangyin Chushi high-dose group were decreased,and the indexes in Qingre Yangyin Chushi high-dose group were the lowest(all P<0.05).The protein expressions of NLRP3,Caspase-1,IL-1β,LC3B,p62 and PHB2 in synovial tissue of rats in Qingre Yangyin Chushi low-dose group and Qingre Yangyin Chushi high-dose group were lower than those in the model group(all P<0.05).Conclusion:Qingre Yangyin Chushi Wan can alleviate synovial tissue inflammation and improve joint function in gout rats by inhibiting the formation of NLRP3 inflammasomes and occurrence of mitochondrial related autophagy.

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备注/Memo

备注/Memo:
基金项目:北京市属医院科研培育计划项目(PZ2019018); 北京中医医院院级课题(LY201803)
更新日期/Last Update: 2023-11-09