[1]黄仁燕,王宏飞,王徐红,等.紫朱软膏对糖尿病溃疡小鼠创面炎症反应及上皮-间充质转化的影响[J].陕西中医,2023,(12):1673-1677.[doi:DOI:10.3969/j.issn.1000-7369.2023.12.002]
　HUANG Renyan,WANG Hongfei,WANG Xuhong,et al.Effect of Zizhu ointment on wound inflammation and epithelial-mesenchymal transformation of diabetic ulcer[J].,2023,(12):1673-1677.[doi:DOI:10.3969/j.issn.1000-7369.2023.12.002]
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紫朱软膏对糖尿病溃疡小鼠创面炎症反应及上皮-间充质转化的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2023年12期
页码:
1673-1677
栏目:
基础研究
出版日期:
2023-12-05

文章信息/Info

Title:
Effect of Zizhu ointment on wound inflammation and epithelial-mesenchymal transformation of diabetic ulcer
作者:
黄仁燕; 王宏飞; 王徐红; 柳国斌
(上海中医药大学附属曙光医院血管外科,上海 201203)
Author(s):
HUANG Renyan; WANG Hongfei; WANG Xuhong; LIU Guobin
(Department of Vascular Surgery,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
关键词:
糖尿病溃疡;  紫朱软膏;  炎症反应;  创面愈合;  白介素;  上皮-间充质转化
Keywords:
Diabetic ulcer;  Zizhu ointment;  Inflammatory response;  Wound healing;  Interleukin;  Epithelial-mesenchymal transformation
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7369.2023.12.002
文献标志码:
A
摘要:
目的:观察紫朱软膏对糖尿病溃疡小鼠创面炎症反应及上皮-间充质转化的影响。方法:采用C57B/L小鼠分别制备为普通溃疡模型和糖尿病溃疡模型,分为对照组、模型组、紫朱软膏组,连续干预14 d,观察小鼠创面愈合情况及愈合率。干预后采用qRT-PCR检测局部溃疡组织炎症相关因子白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、一氧化氮合酶(iNOS)、白介素-4(IL-4)、白介素-10(IL-10)的表达情况,采用Western blot、qRT-PCR方法检测上皮-间充质转化相关细胞因子锌指转录因子(Snail)、α-平滑肌肌动蛋白(α-SMA)、上皮细胞钙黏蛋白(E-cadherin)、β-连环蛋白(β-catenin)表达情况。结果:干预11、14 d,紫朱软膏组小鼠溃疡创面愈合率高于模型组(均P<0.01)。与模型组比较,紫朱软膏组小鼠溃疡创面组织IL-6、TNF-α、iNOS RNA表达降低,IL-4、IL-10 RNA表达升高(均P<0.05)。与模型组比较,紫朱软膏组小鼠溃疡创面组织snail、β-catenin、α-SMA表达上调,E-cadherin表达下调(均P<0.05)。结论:紫朱软膏可能通过减轻炎症反应,诱导上皮-间充质转化,促进糖尿病溃疡愈合。
Abstract:
Objective:To observe the effect of Zizhu ointment on wound inflammation and epithelium-mesenchymal transformation in diabetic ulcer mice.Methods:Common ulcer model and diabetic ulcer model were prepared by C57B/L mice,which were divided into control group,model group and Zizhu ointment group.The wound healing and healing rate were observed after continuous intervention for 14 days.After intervention,the expression of inflammation-related factors IL-6,TNF-α,iNOS,IL-4,IL-10 in local ulcer tissues was detected by qRT-PCR.The expression of epithelial-mesenchymal transformation related cytokines Snail,α-SMA,E-cadherin,β-catenin was detected by Western blot and qRT-PCR.Results:After 11 and 14 days of intervention,the healing rate of ulcer wound in Zizhu ointment group was higher than that in model group(all P<0.01).Compared with model group,the expression of IL-6,TNF-α and iNOS RNA in ulcer wound tissue of mice in Zizhu ointment group decreased,while the expression of IL-4 and IL-10 RNA increased(all P<0.05).Compared with model group,the snail,β-catenin and α-SMA expressions in ulcer wound tissue of mice in Zizhu ointment group were up-regulated,and E-cadherin expressions were down-regulated(all P<0.05).Conclusion:Zizhu ointment may promote the healing of diabetic ulcer by reducing inflammatory response and inducing epithelial-mesenchymal transformation.

参考文献/References:

[1] Schaper NC,Netten JJ,Apelqvist J,et al.Practical guidelines on the prevention and management of diabetic foot disease(IWGDF 2019 update)[J].Diabetes Metab Res Rev,2020,36(S1):e3266.
[2] 刘海艳,张春玲,赵 伟,等.基于PINK1/Parkin信号通路探讨丹黄散在糖尿病小鼠溃疡创面愈合中的作用[J].陕西中医,2023,44(4):433-436.
[3] Thorud JC,Plemmons B,Buckley CJ,et al.Mortality after nontraumatic major amputation among patients with diabetes and peripheral vascular disease:A systematic review[J].The Journal of Foot and Ankle Surgery,2016,55(3):591-599.
[4] Rathnayake A,Saboo A,Malabu UH,et al.Lower extremity amputations and long-term outcomes in diabetic foot ulcers:A systematic review[J].World J Diabetes,2020,11(9):391-399.
[5] Chang M,Nguyen T.Strategy for treatment of infected diabetic foot ulcers[J].Acc Chem Res,2021,54(5):1080-1093.
[6] Armstrong DG,Swerdlow MA,Armstrong AA,et al.Five year mortality and direct costs of care for people with diabetic foot complications are comparable to cancer[J].J Foot Ankle Res,2020,13(1):16.
[7] Awasthi A,Singh SK,Kumar B,et al.Treatment strategies against diabetic foot ulcer:Success so far and the road ahead[J].Curr Diabetes Rev,2021,17(4):421-436.
[8] Wang A,Lv G,Cheng X,et al.Guidelines on multidisciplinary approaches for the prevention and management of diabetic foot disease(2020 edition)[J].Burns Trauma,2020,8(1):1-52.
[9] Chesko DM,Wilgus TA.Immune cells in cutaneous wound healing:A review of functional data from animal models[J].Int J Mol Sci,2022,23(5):2444.
[10] Perez-Favila A,Martinez-Fierro ML,Rodriguez-Lazalde JG,et al.Current therapeutic strategies in diabetic foot ulcers[J].Medicina,2019,55(11):714.
[11] 孙之中,刘朝红,陈其华,等.象皮生肌膏对糖尿病足溃疡大鼠创面组织内上皮-间质转化过程的影响[J].陕西中医,2022,43(12):1664-1668.
[12] 黄仁燕,闫少庆,杨 晓,等.紫朱软膏治疗非缺血型糖尿病足溃疡的临床疗效及对血清中Th1/Th2细胞的影响[J].世界科学技术-中医药现代化,2022,24(4):1586-1592.
[13] 曹烨民.“脱疽”的历史演变及现代意义[J].血管与腔内血管外科杂志,2019,5(6):540-544.
[14] 韩铝洲,吴 清,郑军状,等.金中梁运用五体理论辨治脱疽经验[J].浙江中医杂志,2022,57(10):721-722.
[15] 章 威.中华图书集成:第十八册[M].北京:中医古籍出版社,1999:119.
[16] 黄仁燕,樊炜静,柳国斌.中药油膏治疗糖尿病足溃疡用药规律研究[J].海南医学院学报,2021,27(4):302-306.
[17] 黄仁燕,杨 晓,胡啸明,等.中药油膏治疗糖尿病足溃疡概述[J].山东中医杂志,2020,39(6):635-639.
[18] 黄仁燕,闫少庆,杨 晓,等.紫朱软膏治疗非缺血型糖尿病足溃疡临床疗效及其对细胞免疫水平的影响[J].山东中医杂志,2022,41(10):1096-1101.
[19] Debnath P,Huirem RS,Dutta P,et al.Epithelial-mesenchymal transition and its transcription factors[J].Biosci Rep,2022,42(1):1-29.
[20] 闫 萌,张 征,邢保健,等.胃癌组织中趋化素样因子超家族8蛋白表达与胃癌上皮-间质转化关系及临床意义[J].陕西医学杂志,2023,52(5):618-621,625.
[21] Marconi GD,Fonticoli L,Rajan TS,et al.Epithelial-mesenchymal transition(EMT):The type-2 EMT in wound healing,tissue regeneration and organ fibrosis[J].Cells,2021,10(7):1587.
[22] Han W,Chen M,Li M,et al.Acclimatized induction reveals the multipotency of adult human undifferentiated keratinocytes[J].Cell Reprogram,2010,12(3):283-294.
[23] Vu R,Dragan M,Sun P,et al.Epithelial-mesenchymal plasticity and endothelial-mesenchymal transition in cutaneous wound healing[J].Cold Spring Harb Perspect Biol,2022,12(1):41237.
[24] Arun PR,Kamakshi B,Sayanti D,et al.Silk sponges ornamented with a placenta-derived extracellular matrix augment full-thickness cutaneous wound healing by stimulating neovascularization and cellular migration[J].ACS Appl Mater Interfaces,2018,10(20):16977-16991.
[25] 宋 宇,包秀丽.JNK与TGF-β、非经典Wnt信号通路在眼部纤维化中的研究进展[J].陕西医学杂志,2023,52(2):233-237.
[26] Manfioletti G,Fedele M.Epithelial-mesenchymal transition(EMT)2021[J].Int J Mol Sci,2022,23(10):5848.
[27] Haensel D,Dai X.Epithelial-to-mesenchymal transition in cutaneous wound healing:Where we are and where we are heading[J].Dev Dyn,2018,247(3):473-480.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82274528,82305245); 上海市“科技创新行动计划”医学创新研究专项课题项目(21Y21920200); 上海市浦东新区卫生健康委员会浦东名中医培养计划项目(PWRZM2020-18); 上海中医药大学附属曙光医院四明青年基金资助项目(SGKJ-202119)
更新日期/Last Update: 2023-12-08