[1]宋晨曦,张 鼎,胡芷涵,等.温肺降浊方对血管性痴呆模型大鼠ERK1/2信号通路相关蛋白的影响[J].陕西中医,2024,(2):171-175,186.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.005]
　SONG Chenxi,ZHANG Ding,HU Zhihan,et al.Effects of Wenfei Jiangzhuo prescription on ERK1/2 signaling pathway in vascular dementia rats brain tissue[J].,2024,(2):171-175,186.[doi:DOI:10.3969/j.issn.1000-7369.2024.02.005]
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温肺降浊方对血管性痴呆模型大鼠ERK1/2信号通路相关蛋白的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
171-175,186
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effects of Wenfei Jiangzhuo prescription on ERK1/2 signaling pathway in vascular dementia rats brain tissue
作者:
宋晨曦1; 张 鼎1; 胡芷涵2; 姜明贺1; 李方存1; 胡跃强3
(1.广西中医药大学,广西 南宁 530001; 2.上海中医药大学,上海 201203; 3.广西中医药大学第一附属医院,广西 南宁 530023)
Author(s):
SONG Chenxi; ZHANG Ding; HU Zhihan; JIANG Minghe; LI Fangcun; HU Yueqiang
(Guangxi University of Chinese Medicine,Nanning 530001,China)
关键词:
血管性痴呆;  温肺降浊方;  ERK1/2信号通路;  海马组织;  大鼠
Keywords:
Vascular dementia;  Wenfei Jiangzhuo prescription;  ERK1/2 signaling pathway;  Hippocampal tissue;  Rats
分类号:
R 749.1
DOI:
DOI:10.3969/j.issn.1000-7369.2024.02.005
文献标志码:
A
摘要:
目的:观察温肺降浊方对血管性痴呆(VaD)模型大鼠细胞外调节蛋白激酶(ERK1/2)信号通路的影响,探讨其治疗VaD可能的相关作用机制。方法:将雄性SD大鼠随机分为假手术组和模型组(等体积0.9%氯化钠溶液灌胃),温肺降浊方低、中、高剂量组(1.5、3、6 ml剂量灌胃)。观察造模后大鼠水迷宫评分和HE病理变化,免疫组化、RT-qPCR和Western blot检测海马组织中ERK1/2、钙蛋白酶(Calpain)、Bcl-2关联死亡启动子重组蛋白(Bad)、B淋巴细胞瘤-xl(Bcl-xl)蛋白和mRNA表达情况。结果:与假手术组相比,模型组和温肺降浊方各剂量组大鼠逃避潜伏期延长、穿越平台次数缩短(P<0.05),海马组织形态稀疏、水肿及核缩小; 海马组织ERK1/2、Calpain、Bad蛋白和mRNA表达升高(P<0.05),Bcl-xl蛋白和mRNA表达下降(P<0.05)。与模型组比较,温肺降浊方高剂量组大鼠逃避潜伏期缩短、穿越平台次数增加(P<0.05),海马组织形态逐渐紧密、水肿减少,数量增多; 海马组织ERK1/2、Calpain、Bad蛋白和mRNA表达下降(P<0.05),Bcl-xl蛋白和mRNA表达升高(P<0.05)。结论:血管性痴呆可能与大鼠海马中的ERK1/2、Calpain、Bad蛋白升高及Bcl-xl蛋白降低有关,温肺降浊方可以抑制VaD大鼠海马中的ERK1/2通路激活,减少神经细胞凋亡,延缓疾病进展,改善血管性痴呆大鼠的学习记忆能力。
Abstract:
Objective:To observe the effect of Wenfei Jiangzhuo prescription on ERK1/2 signaling pathway in Vascular dementia(VaD)model rats,and to explore the possible mechanism of its therapeutic effect on VaD.Methods:Male SD rats were randomly divided into the following groups:the sham operation group and the model group(equal volume of saline gavage),the low-dose group of Wenfei Jiangzhuo prescription,the medium-dose group of Wenfei Jiangzhuo prescription and the high-dose group of Wenfei Jiangzhuo prescription(1.5,3 and 6 ml doses by gavage).The water maze score and HE pathological changes of rats after modeling were observed,and ERK1/2,Calpain,Bad,Bcl-xl protein and mRNA expressions in hippocampal tissues were observed by immunohistochemistry,RT-qPCR and Western blot.Results:Compared with the sham-operated group,rats in the model group and each dose group of Wenfei Jiangzhuo prescription had prolonged evasion latency and shortened the number of crossing platforms(P<0.05),and the morphology of hippocampal tissues was sparse,edematous,and the nuclei were shrunken; the expression of ERK1/2,Calpain,and Bad proteins and mRNAs in hippocampal tissues was elevated(P<0.05)and the expression of Bcl-xl proteins and mRNAs was decreased(P<0.05); compared with the model group,rats in the high-dose group of Wenfei Jiangzhuo prescription had shorter evasion latency and increased the number of crossing platforms(P<0.05),the morphology of hippocampal tissues was progressively tighter,the edema was reduced,and the number of hippocampal tissues was increased; the hippocampal tissues had decreased in the expression of ERK1/2,Calpain,and Bad proteins and mRNAs(P<0.05),and the expression of Bcl-xl proteins and mRNAs was elevated(P<0.05).Conclusion:Vascular dementia may be related to the elevation of ERK1/2,Calpain and Bad proteins and the decrease of Bcl-xl protein in the hippocampus of rats.Wenfei Jiangzhuo prescription can inhibit the activation of ERK1/2 pathway in the hippocampus of rats with VaD,reduce the apoptosis of neuronal cells,slow down the progression of the disease,and improve learning and memory ability of rats with vascular dementia.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82260904); 广西中医脑病临床研究中心项目(桂科AD20238028); 广西高等学校高水平创新团队及卓越学者计划项目(桂教人才202006); 广西中医药大学博士研究生创新项目(YCBXJ2023010)
更新日期/Last Update: 2024-02-07