[1]易 林,向文远,张文豪,等.补肾痹通方联合骨髓间充质干细胞对损伤软骨细胞的保护机制及SOX9、MMP-13表达的影响[J].陕西中医,2024,(6):728-732,739.[doi:DOI:10.3969/j.issn.1000-7369.2024.06.002]
　YI Lin,XIANG Wenyuan,ZHANG Wenhao,et al.The protective mechanism of Bushen Bitong recipe combined with bone marrow mesenchymal stem cells on injured chondrocytes and its effects on the expression of SOX9 and MMP-13[J].,2024,(6):728-732,739.[doi:DOI:10.3969/j.issn.1000-7369.2024.06.002]
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补肾痹通方联合骨髓间充质干细胞对损伤软骨细胞的保护机制及SOX9、MMP-13表达的影响
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《陕西中医》[ISSN:1000-7369/CN:61-1281/TN]

卷:
期数:
2024年6期
页码:
728-732,739
栏目:
基础研究
出版日期:
2024-06-05

文章信息/Info

Title:
The protective mechanism of Bushen Bitong recipe combined with bone marrow mesenchymal stem cells on injured chondrocytes and its effects on the expression of SOX9 and MMP-13
作者:
易 林1; 2; 向文远2; 张文豪1; 2; 石正誉1; 2; 热米拉·艾买提1; 2; 邓迎杰2; 方 锐2
(1.新疆医科大学第四临床医学院,新疆 乌鲁木齐 830054; 2.新疆医科大学附属中医医院,新疆 乌鲁木齐 830099)
Author(s):
YI Lin; XIANG Wenyuan; ZHANG Wenhao; SHI Zhengyu; AIMAITI·Remila; DENG Yingjie; FANG Rui
(The Fourth Clinical Medical College of Xinjiang Medical University,Urumqi 830054,China)
关键词:
骨关节炎;  补肾痹通方;  骨髓间充质干细胞;  软骨细胞;  性别决定区Y框蛋白9;  基质金属蛋白酶13
Keywords:
Osteoarthritis;  Bushen Bitong recipe;  Bone mesenchymal stem cells;  Chondrocytes;  Sex determining region Y box protein 9;  Recombinant matrix metalloproteinase 13
分类号:
R 684.3
DOI:
DOI:10.3969/j.issn.1000-7369.2024.06.002
文献标志码:
A
摘要:
目的:探讨补肾痹通方(BSBT)联合骨髓间充质干细胞(BMSCs)对白介素(IL)-1β诱导的损伤软骨细胞的保护机制及性别决定区Y框蛋白9(SOX9)、基质金属蛋白酶13(MMP-13)表达的影响。方法:使用10 ng/ml的IL-1β建立损伤软骨细胞模型,实验分为对照组、模型组(IL-1β)、中药组(IL-1β+BSBT)、干细胞组(IL-1β+BMSCs)和联合组(IL-1β+BSBT+BMSCs); CCK-8法检测各组软骨细胞增殖情况; RT-qPCR法和Western blot法分别检测软骨细胞内SOX9、MMP-13、Ⅱ型胶原α1重组蛋白(COL2A1)、IL-10的基因和蛋白表达; ELISA检测各组细胞培养上清中肿瘤坏死因子-α(TNF-α)、IL-6、胰岛素样生长因子1(IGF-1)、碱性成纤维细胞生长因子(bFGF)的水平。结果:与模型组比较,各组软骨细胞活性显著增强(P<0.01),软骨细胞中的SOX9、COL2A1、IL-10的基因和蛋白水平显著升高(P<0.01),MMP-13的基因和蛋白水平明显降低(P<0.01),软骨细胞培养上清中TNF-α、IL-6的含量显著降低(P<0.01),IGF-1、bFGF的水平升高(P<0.01),其中联合组变化最明显(P<0.05)。结论:BSBT联合BMSCs可有效保护损伤软骨细胞,其机制可能是通过上调SOX9,下调MMP-13,抑制炎症,改善软骨细胞微环境,促进关节软骨的修复与再生。
Abstract:
Objective:To explore the protective mechanism of Bushen Bitong recipe(BSBT)combined with bone marrow mesenchymal stem cells(BMSCs)on IL-1β-induced injured chondrocytes,as well as the effects on the expression of SOX9 and MMP-13.Methods:An injured chondrocyte model was established using 10 ng/ml of IL-1β,and the experiments were divided into control group,model group(IL-1β),traditional Chinese medicine group(IL-1β+BSBT),stem cell group(IL-1β+BMSCs),and combined group(IL-1β+BSBT+BMSCs).CCK-8 assay for chondrocyte proliferation in each group.And gene and protein expression of SOX9,MMP-13,and IL-10 in chondrocytes by RT-qPCR and Western blot,respectively.The levels of TNF-α,IL-6,IGF-1,and bFGF in the cell culture supernatants of each group were detected by ELISA.Results:Compared with the model group,the chondrocyte activity was highly significantly enhanced in all groups(P<0.01),the gene and protein levels of SOX9,COL2A1 and IL-10 in chondrocytes were highly significantly elevated(P<0.01),and the gene and protein levels of MMP-13 were highly significantly reduced(P<0.01),and the content levels of TNF-α and IL-6 in the supernatants of chondrocyte culture were highly significantly decreased(P<0.01),and the content levels of IGF-1 and bFGF were highly significantly increased(P<0.01),with the best performance in the combined group(P<0.05).Conclusion:BSBT combined with BMSCs effectively protected injured chondrocytes,possibly by up-regulating SOX9 and down-regulating MMP-13,inhibiting inflammation,improving the microenvironment of chondrocytes,and promoting the repair and regeneration of articular cartilage.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82360934); 新疆维吾尔自治区自然科学基金资助项目(2022D01C170,2022D01C171); 新疆维吾尔自治区科技创新领军人才项目(2022TSYCLJ0007); 新疆维吾尔自治区重点研发任务专项课题(2021B03006-2)
更新日期/Last Update: 2024-06-11